Gluconeogenesis

Question 1

What cells undergo gluconeogenesis?

Answer 1

Liver and kidney cells only

Primarily liver cells though

Question 2

What precursors does gluconeogenesis use?

Answer 2

Noncarbohydrate precursors (mainly amino acids)

Question 3

Irreversible reactions/enzymes of gluconeogenesis

Answer 3

-Glucose 6-phosphatase 1st step

(G6-P -> Glucose)

-Fructose 1,6 biphosphatase 2nd step

F1,6-BP -> F6-P

• Pyruvate carboxylase 3rd step

Pyruvate - > OAA

• PEP carboxykinase 3rd step

OAA -> PEP

Question 4

Why are there 4 irreversible reactions in gluconeogenesis, but only 3 irreversible reactions in glycolysis?

Answer 4

Pyruvate -> PEP requires two enzymes since PEP is very high energy molecule

Question 5

Net products of gluconeogenesis

Answer 5

1 Glucose

2 NAD+

Question 6

Net reactants of Gluconeogenesis

Answer 6

2 pyruvate

2 NADH
4 ATP and 2 GTP (used in conversion of pyruvate to PEP)

Question 7

What intermediate moves in Gluconeogenesis?

Answer 7

OAA moves from matrix to cytoplasm indirectly