Fatty Acid Oxidation

Question 1

Where does fatty acid oxidation occur?

Answer 1

Mitochondrial matrix

Question 2

Net products of Fatty Acid Oxidation

Answer 2

Acetyl-CoA
NADH
FADH2

Removes 2 carbon fragments from the carboxyl end of a fatty acyl CoA

Question 3

Acyl-CoA Dehydra

Answer 3

Enzyme that catalyses the first step of FA oxidation

• dehydrogenation of two carbon atoms to form FADH2
• generates a trans double bond between C2-C3

Question 4

delta-Enoyl-CoA hydratase

Answer 4

Enzyme that catalyses the 2nd step of FA oxidation

• uses water to add a hydroxyl group to the C3 of the trans double bond
• forms hydroxyl FA

Question 5

3-HA-CoA dehydra

Answer 5

Enzyme that catalyses the 3rd step of FA oxidation

• removes 2 hydrogens to form NADH and Keto FA

Question 6

3-KA-CoA Thiolase

Answer 6

Enzyme that catalyzes the 4th step of FA oxidation

• removes acetyl CoA group from fatty acid
• spontaneously adds a free CoA group
• net result is a 2 carbon less fatty acid CoA

Question 7

Palmitic acid produces how many acetyl CoA

Answer 7

8

And 7 NADH/FADH2

Question 8

How is FA oxidation stopped?

Answer 8

No oxygen present

• requires ETC and CTC to be working

Question 9

Pre-step in FA oxidation

Answer 9

Acrylic-CoA synthase binds a CoA SH via thioester linkageto a fatty acid via ATP in the cytoplasm of the mitochondria

Question 10

Carnations shuttle

Answer 10

Shuttles CoA-SH fatty acid from cytoplasm to the matrix to undergo FA oxidation

• rate limiting step of FA oxidation

Question 11

Why is energy stored as fat over carbs/protein

Answer 11

Fat gives a higher energy yield per unit weight as long as aerobic respiration is occurring

Fats also have higher calorie count (9 per g)

Question 12

What do fatty acids use to enter the cells from blood?

Answer 12

Albumin