Genetics Quiz Flashcards
purpose of the shampoo is? What is the purpose of the NaCl?
Shampoo denatures proteins
Salt separates proteins from DNA
What does mushing do
Releases DNA, similar to extraction of DNA in cells
Why do we cool the mixture?
Cooling slows down the reaction
Acts as a filter
What is being filtered out? What is going through the filter?
Cell debris and protein are being filtered out.
DNA and extraction pass through.
What do you think the ethanol does ? Why do we want it cold?
Ethanol takes DNA out of the solution
DNA isn’t soluble so it can be seen
What do you see in the top portion of the liquid?
Strands of DNA
visible because it’s not dissolved, due to cold ethanol
Would there be any difference if you had extracted DNA from an animal cell, instead of a plant cell?
Plants have a cell wall so it would be harder
Both involve breaking down and denaturing proteins.
Purpose of lab
purification and isolation of DNA from other cellular materials.
If we want to isolate DNA from kiwifruit, what do we have to get rid of?
Everything but the dna. Protein, fat, carbohydrates
What can we do with the DNA once we’ve purified it?
Study genetics
Create new medicines
Genetically modify crops
What is dna insoluable in
Dna is insoluble in non polars, ethanol. When mixed precipitates out of solvent
What does DN ase do to the body
Breakes down dna molecules
Brakes down waste
