genetics Flashcards

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1
Q

what is a nucleotide

A

-they form DNA
-nucleotides are the monomers, DNA is the polymer

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2
Q

what is a strand

A

many nucleotides joined together

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3
Q

what are the 3 parts of a nucleotide

A

phosphate, deoxyribose sugar, base (the base is the part that varies)

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4
Q

how to extract DNA from peas

A

-mix salt water and washing up liquid in a beaker
-mash peas to separate cells
-add to beaker
-transfer into boiling tube and put into hot water bath
-strain mixture
-place filtered juice into a boiling tube
-add enzyme solution to break down proteins holding DNA
-add ethanol slowly (50/50 ethanol to juice)
-White layer of DNA forms between juice and ethanol

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5
Q

allele

A

different forms of the same gene

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6
Q

phenotype

A

an organisms outward characteristics

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7
Q

genotype

A

the genes/alleles of an organism

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8
Q

heterozygous

A

having 2 different alleles for a particular characteristic

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9
Q

homozygous

A

having the same allele for a particular characteristic

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10
Q

dominant allele

A

an allele which has an effect in both heterozygous and homozygous

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11
Q

pure breeding

A

an organism is homozygous so only one type of allele can be passed onto offspring

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12
Q

punnet square

A

diagram showing possible genotypes and phenotypes which could form for offspring

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13
Q

what are gametes

A

sex cells

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14
Q

define meiosis

A

-cell division producing four non-identical, haploid gametes
-cells divide twice producing 4 unique daughter cells

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15
Q

What genotype produces a male baby

A

XY

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16
Q

What genotype produces a female baby

A

XX

17
Q

what causes mutations

A

the wrong base may be inserted into the new DNA molecule

18
Q

cloning an explant with tissue culture

A

-clean surfaces with ethanol
-cut a small explant off of a cauliflower
-sterilise explants in sodium and swirl every few minutes
-strain explants out from liquid
-place into agar plant growth medium stalk end first
-incubate in warm place with light
-explant should grow leaves and get larger