Genetics Flashcards
What stage does Synapsis occur in meiosis ?
Prophase I
What genetic configuration disrupts Mendel’s law of genetics?
Linked genes on the same chromosome
What are the conditions needed for Hardy Weinberg equations to hold true?
- no mutations
- no natural selection
- Random mating
- Large Population
- no migration
how many generations are required to restore Hardy Weinberg equilibrium if disrupted?
1 generation
When can natural selection occur?
if there is genetic diversity
How does hardy Weinberg impact allele frequencies?
The Hardy-Weinberg theory states that allele frequencies will be UNCHANGING if certain conditions are met
Describe the components of a genome
Human genome:
- has 23 pairs of chromosomes (46 total chromosomes), comprised of 22 autosomal pairs and 1 pair of sex chromosomes (XX or XY)
-Each gene has only 1 locus.
There are 4 RNA bases (U, C, G, A) and 64 possible codons.
What is ELISA used for?
ELISA (Enzyme-linked imunno absorbent assay)
-ELISA uses fluorescent or enzyme-linked antibodies to find either antigen or other antibodies in a person’s serum
What is western blotting?
Western blots use antibodies to detect specific PROTEINS in a given sample of tissue extract.
Gel electrophoresis is used to separate native or denatured proteins by the length and charge of the polypeptide (
What is PCR Amplification ?
PCR amplification allows you to take a specific sequence of DNA and make multiple copies of it.
What is FISH (lab technique) used for?
FISH can identify a range of mutations including deletions, duplications, aneuploidy, and the presence of derivative chromosomes
(Flourescence in situ hybridization)
What is a test cross?
A testcross is used to determine whether an individual with a dominant trait (and an unknown genotype) is heterozygous or homozygous
What is a back cross ?
back”cross implies that the individual is crossed “back” to a member of the previous generation
What is the result of inbreeding?
inbreeding results in an increase in the frequencies of all homozygous genotypes,
What is effect of assortive mating ?
while assortive mating only increases the frequency of homozygosity in the trait being selected for