Genetic Technology: Restriction Analysis & Recombinant DNA

Question 1

Genetic Engineering

Answer 1

The manipulation of an organism’s DNA with another organism’s DNA

Question 2

Restriction Enzymes

Answer 2

• AKA: Endonuclease - proteins used to cut up DNA

* Made by bacteria, used as a defense by viruses (by chopping up invading viruses)

Question 3

How Restriction Enzymes Work

Answer 3

• the proteins look for specific short DNA sequences (recognition sites)
• once the site is found the enzymes cuts the DNA in or near that sequence
• the types of cut depends on the site

Question 4

Recombinant DNA

Answer 4

the DNA that results when the DNA from two DIFFERENT organisms is joined

Question 5

Human Gene Therapy

Answer 5

• alteration of an individual’s DNA for medical benefit

* DNA can be added directly or by adding transgenic cells