Genes and Cell markers

Question 1

T-ALL gene mutations

Answer 1

GOF of NOTCH1 (70%)

Question 2

B-ALL gene mutations?

Translocation?

Answer 2

LOF of PAX5, E2A, and EBV

-t(12;21) . . ETV6 and RUNX1

Question 3

CD21

Answer 3

EBV receptor; mature B cells and follicular dendritic cells

Question 4

CD23

Answer 4

Activated mature B cells

Question 5

CD79a

Answer 5

marrow Pre B cells and mature B cells

Question 6

CD11c

Answer 6

Granulocytes, monocytes, and macrophages; also expressed by hairy cell leukemias

Question 7

CD13

Answer 7

immature and mature monocytes and granulocytes

Question 8

CD14

Answer 8

monocytes

Question 9

CD15

Answer 9

Granulocytes; Reed-Sternburgs cells

Question 10

CD33

Answer 10

Myeloid progenitors and monocytes

Question 11

CD64

Answer 11

Mature myeloid cells

Question 12

CD16

Answer 12

NK cells and granulocytes

Question 13

CD56

Answer 13

NK cells and a subset of T cells

Question 14

CD34

Answer 14

Pluripotent HSC and progenitor cells of many lineages

Question 15

CD30

Answer 15

Activated B cells, T cells, and monocytes; Reed Sternberg cells

Question 16

CD45

Answer 16

ALL LEUKOCYTES; also known as leukocyte common antigen (LCA)

Question 17

t(15;17)

Answer 17

Acute promyelocytic leukemia

Question 18

Distinctive immunophenotype of CLL/SLL

Answer 18

• Pan B cell markers CD19, CD20, CD23, and CD5

- also low level expression of surgace Ig (usually IgM or IgM and IgD)

Question 19

Gene overexpression and translocation for follicular lymphoma

Answer 19

• BCL2

- t(14;18)

Question 20

Immunophenotype of follicular lymphoma

Answer 20

• CD19, CD20, CD10, Surface Ig, and BCL6
• unlike CLL/SLL and mantle cell lymphoma, CD5 is NOT expressed
• BCL2 in more than 90%
• normal follicular center B cells are BCL2-NEGATIVE

Question 21

Dysregulation in DLBCL

Answer 21

• often BCL6
• small % have t(14;18) @ BCL2 . . . these are probably follicular lymphomas that transformed and are now more aggressive

Question 22

Translocation for Burkitt lymphoma

Answer 22

c-MYC oncogene on chromosome 8 . . .ALL HAVE THIS

-virtually always fail to express the anti-apoptotic protein BCL2

Question 23

immunophenotype of Burkitt lymphoma

Answer 23

-surface IgM, CD19, CD20, CD10, BCL6

Question 24

Immunophenotype for MM

Answer 24

• CD138 (syndecan-1)

- CD56

Question 25

mutations for lymphoplasmacytic lymphoma

Answer 25

MYD88

Question 26

translocation for Mantle Cell lymphoma

Answer 26

• (11;14) translocation

- this involves IgH locus on chomosome 14 and the cyclin D1 locus on chromosome 11

Question 27

Tranlocations acquired in Marginal Zone lymphoma?

-What do they upregulate

Answer 27

• (11;18), (14;18), or (1;14)

- BCL10 or MALT1

Question 28

Mutation and surface markers for Hairy cell leukemia?

Answer 28

• BRAF
• surface IgG
• CD11c, CD25, CD103

Question 29

Immunophenotype Peripheral T cell lymphoma, unspecified

Answer 29

-CD2, 3, 5, and some CD4 or 8

Question 30

what 2 neoplasms are CD30+

Answer 30

Anaplastic Large Cell Lymphoma (ALK pos) and Hodgkins lymphoma

Question 31

Gene mutation associated with Large Granular lymhocytic leukemia

Answer 31

STAT3

Question 32

Cell markers for Langerhans Cell histiocytosis

Answer 32

• S-100+
• CD1a
• HLA-DR