General Biology 2 Flashcards
Carries the genetic information in all
living organisms.
DNA
a single stranded type of nucleic acids
that is mostly involved in protein synthesis
RNA
is a protein molecule that catalyzes
biochemical reaction.
enzymes
is the molecule that carries genetic information for the development and functioning of an organism
Deoxyribonucleic Acid
is a process that uses laboratory-based technologies to alter the DNA makeup of
an organism
Genetic Engineering
breeding involves selecting a parent that have characteristics of interest
Classical Breeding
STEPS IN CLASSICAL BREEDING
- Determine which trait are significant enough to be chosen.
- Select parent that exemplify these traits
- Choose the best offspring from parents to produce the next generation.
- Repeat the process.
The technology used for producing artificial
DNA through the combination of different genetic materials (DNA) from different sources.
Recombinant DNA Technology
STEPS IN CREATING A RECOMBINANT DNA
- Cutting or cleaving of DNA and plasmid by “restriction enzyme”.
- Inserting of gene of interest into the open plasmid by “DNA ligase”.
- Insert the recombinant plasmid back to the bacterial cell.
- Isolation of the gene copies and insertion to other organisms to confer the desired trait.
EXAMPLES OF GMO
- BT CORN- Bacillus thuringiensis, resistant to corn-borer disease.
- BT EGGPLANT - Bacillus thuringiensis,
resistant to eggplant fruit and shoot borer - GOLDEN RICE - ß (beta) carotene, pre
cursor Vitamin A. - HUMAN INSULIN - People with type I
diabetes could not synthesize their own insulin. Thus, they need to inject themselves daily
made up of sequences of DNA and are arranged, one after another, at specific locations on chromosomes in the nucleus of cells.
GENE
Different version of a gene resulting in contracting effect on a characteristic, e.g. tallness and shortness.
ALLELES
For each character, an organism inherits two alleles, one from each parent
Genes are not alleles, but alleles are genes
An individual with identical alleles for a gene; also called PURE BREED
HOMOZYGOUS
An individual with 2 different alleles for a gene.
HETEROZYGOUS