Gene Modification Process Flashcards
1
Q
What are the steps of producing recombinant DNA ?
A
Isolation of the gene
Inserting the gene into a vector for transfer
Getting recombinant DNA into the cell
2
Q
What are the 3 different methods of isolating a gene ?
A
Restriction endonuclease
Reverse transcriptase
Sequence worked out from amino acids sequence and DNA built to match.
3
Q
What are the ways of getting recombinant DNA into a cell?
A
Use of a virus
DNA enclosed in a liposome
Gene guns
Micro injection
4
Q
What is a promotor ?
A
A section of DNA that when it is active instructs a cell to copy the section of DNA after it.
5
Q
What do Marker genes do ?
A
They enable identification of specific genes
