GEN Lecture 2

Question 1

PCR is based on a ____

Answer 1

heat stable DNA polymerase that generates the second strand of DNA from a single stranded template.

Question 2

DNA polymerases require a ___

Answer 2

primer.

Question 3

Reagents in a PCR reaction:

Answer 3

heat stable DNA polymerase, dNTPs, and two primers (one for each strand)

Question 4

3 steps of PCR:

Answer 4

Denaturation, Annealing, Extension

Question 5

Cycle 1 yields _ molecules.

Answer 5

2

Question 6

Cycle 2 yields _ molecules

Answer 6

4

Question 7

Cycle 3 yields _ molecules

Answer 7

8

Question 8

Gel electrophoresis seperates DNA or protein on the basis of ___

Answer 8

basis of their rate of movement through a gel in an electrical field - molecular sieve.

Question 9

Transcriptome:

Answer 9

The part of the genome that is transcribed into RNA, particularly mRNA.