GEN Lecture 1 Flashcards

1
Q

Recombinant DNA

A

Genes or DNA from two dfiferent sources are combined in vitro into the same molecule.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

A typical gene has two sections:

A

The promoter, which controls transcription of mRNA, and the coding region

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Which of the eukaroyte promoter and prokaryote promoter will be used?

A

The prokaryotic one will be used.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

The coding region of the __ will be swapped for the coding region of the ___

A

Eukaryote will be swapped for prokaryote.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

What is biotechnology?

A

The manipulation of organisms or their components to make useful products.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Genetic engineering manipulates ____

A

DNA in vitro.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Selective breeding vs Genetic Engineering control of gene expression:

A

No control in selective breeding, control in genetic engineering

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Genetic engineering vs selective breeding: genes from ___

A

GE: Any species, SB: Only genes from related species

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Choice of genes, GE vs SB

A

GE: Precise choice of one or a few genes, SB: New combos of many genes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

DNA ligase:

A

seals the strands after restriction enzymes.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Two types of markers are required:

A

A marker to make sure that bacterium has taken up the plasmid, and one to make sure that the plasmid has an insert or foreign gene

How well did you know this?
1
Not at all
2
3
4
5
Perfectly