GEN Lecture 1 Flashcards
Recombinant DNA
Genes or DNA from two dfiferent sources are combined in vitro into the same molecule.
A typical gene has two sections:
The promoter, which controls transcription of mRNA, and the coding region
Which of the eukaroyte promoter and prokaryote promoter will be used?
The prokaryotic one will be used.
The coding region of the __ will be swapped for the coding region of the ___
Eukaryote will be swapped for prokaryote.
What is biotechnology?
The manipulation of organisms or their components to make useful products.
Genetic engineering manipulates ____
DNA in vitro.
Selective breeding vs Genetic Engineering control of gene expression:
No control in selective breeding, control in genetic engineering
Genetic engineering vs selective breeding: genes from ___
GE: Any species, SB: Only genes from related species
Choice of genes, GE vs SB
GE: Precise choice of one or a few genes, SB: New combos of many genes
DNA ligase:
seals the strands after restriction enzymes.
Two types of markers are required:
A marker to make sure that bacterium has taken up the plasmid, and one to make sure that the plasmid has an insert or foreign gene
