Fundamental Concepts: Proximate Analysis Flashcards
What is nutrient analysis?
A test done on a feed sample to determine nutrient content using either chemical, biological, or enzymatic methods
What is the wet chemistry method?
Analyses feed sample in liquid form
Original method of feed analysis
More accurate
What is the NIRS method?
Uses light reflect acne to measure a feed sample
Faster and cheaper
More precise
What is proximate analysis?
Determines the composition of a feed sample in terms of nutrients
Who developed the proximate analysis method?
Developed in the 19th century at the Weende experiment station by Henneberg and Stahmann
What are the steps to proximate analysis?
- Take multiple small samples from different locations
- Mix all samples into a large composite sample
- Sum of composite sample is collected
- Do not shake to maintain sample integrity
What does proximate analysis measure?
Dry matter, crude protein, ether extract, ash, crude fiber, and nitrogen free extract
How is dry matter (DM) calculated?
- Feed is dried at 105 degrees Celsius overnight
- Calculate: (dried feed sample/original feed sample) x 100
What is the Kjeldahl method?
Developed by Johan Kjeldahl to determine crude protein (CP) from nitrogen content in a feed sample.
Calculation: N x 6.25
How is ether extract (EE) calculated?
- Boil dried fat content sample in diethyl ether for 4 hours
- Most sample will separate or evaporate
- Leftover residue is weighed
How is ash or total inorganic component calculated?
- Heat sample at 500-700 degrees Celsius for 2-4 hours
- Leftover residue is weighed
How is crude fiber (CF) calculated?
- A dried feed sample is boiled with a solution of 1.25% hydrogen sulfide and then filtered
- Leftover residue is dried and ashes
- Calculation: filtered dried sample weight - ash weight
How is nitrogen free extract (NFE) calculated?
Calculated value is not analyzed only a guess
%NFE = (%DM - (%EE + %CP + %ash + %CF))
What is the van soest analysis method?
An improved method of feed analysis where neutral detergent fiber (NDF) and acid detergent fiber (ADF) is calculated
What composes NDF?
Hemicellulose, cellulose, and lignin
What composes ADF?
ADF protein, lignin, and cellulose