Functions of G coupled receptors Flashcards
Can the G protien compelx be stimultaory or inhibitory
BOTH
What does Adenyly Cyclase Chlorea Toxin cause
Diarrhea
How does Adenyly cyclase cholera toxin create diarrahea
It enters the inside where it is able to bind to the G protien sub unit that stimulates Adenylate cyclase which turns on cAMP and turns on protien kinase A which pumps out chlorine creating a salt where water then needs to be pumped out causing diarrhea
What is Pertuss toxin do
uses whooping cough in the lungs
What state is the G protien in when pertussis toxin
It is in stimulating state because the inactive state is bloced by the PTX toxin so cAMP is always being produced
Where does cAMP bind to PKA first
The B cite and then the A cite
What does cAMP binding to the CNB-B site do
Creates a higher affinity and a lower kD
What detaches from protien kinase
The catalytic site
What are four common intracellular second messengers
- Cyclic AMP
- Cyclic GMP
- Diacylglyceron
- Inositol trisphoshpate
what does cAMP do
Inglycogen metabolism
Activate PKA
What does PKA activate to produce glucose
GPK which then produces GP
What is the inhibtor of phosohoprotien phsophates doing when trying to produce glucose
It is activated to inhbiot phophoprotien phosphate which are molecules that remove phosphates
What is the stimulatory hormone that activates glucose production
epinepherine, glucagon
How do you get glucose back into its glycogen form
Remove the stimulatory hormone
What will removing the stimulatory hormone do
Activate glycogen synthatse because it is no longer phosphorylated by PKA
What is activating phosphorylation for glucose
Glycogen phosphorylase kinase
Glycogen phosphorylase
What is inhiitory phosphorylation for production of glucose
Inactive of glycogen synthase
And the phoshporylation of the inhibitor of phosphoprotien phosphatase
What is active-de phosphorylation of glycogen synthesis
GS when it is not phosphorylated it is in its active form
What is inactive of glycogen breakdown (inhibiton)
GPK and GP is inactive when it is dephoshporylated
What is the genes regulated by PKA have a specefic…
Nucletoide sequence that is known as cAMP response element
What does CREB stand for
CRE-binding protien
How does P-CREB bind
as a dimer to cAMP response element
What does P-CREB also bind too
CBP/P300 coactivator
what does CBP/P300 do
Recruits transcriptional machinery
What plays a major role for synaptic remodeling
CREB
What does Phospholipase C do
Cleaves PIP2 into DAG and IP3
What activates PLC
Certain Ga subunits
When does Phosphilpase cleave
When it is bound to the GTP alpha G subunit
What does IP3 do when released
Opens the Ca channel into the ER
What does calcium do when it is the ER
Attach to the protein kinase which then turns on phosphorylation of substrates
What does Nitroglycerin be used to treat
Angina it decomposes into NO
How does relaxation of muscle cell occur
Acetylechloine is relased which activates GPCR then Phosphlipase C then the IP3 activated the Calcium which creates NO sunthase producing NO onto the receptor creating cGMP then protien kinase G which will have the muscle relax
Who uses vigra mostly
Older people because there blood pressure is higher
How does Vigra work
It works to inhibit the PDE 5 which in turn converts cGMP into GMP this way it stays at cGMP and produces Protien kinase G and relax the muslce
What are side effects of vigra
Priapism (Erection that doesn’t go away to O2 to the penis)
Heart attack
Stroke
Sudden Hearing Loss
Cynaopsia
Decreasing the BP could lead to stroke
