Forensics 1 Flashcards

1
Q

what is toxicology

A

analysis of body fluids and tissues for presences of drugs and poisons

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2
Q

3 areas of forensic toxicology

A

postmortem: cause of death
human performance: drugs/poisons modifying human behaviour or performance
forensic drug testing: determine drug use

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3
Q

knowledge required for a forensic toxicologist

A

drug metabolism
effects of poisoning on body
analytical chemistry
pharmacological action s

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4
Q

toxicology role in death

A
homicide
suicide
accident
natural 
was there a drug, how much, when
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5
Q

does toxicologist determine cause of death

A

no pathologist does

answers if the amount was sufficient to cause death

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6
Q

info required to aid in interpretation in death

A
time and date of death
details of last meal
treated in hospital 
med history
symptoms
evidence at scene
time delay between death and autopsy
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7
Q

what is postmortem redistribution

A

distribution of drugs from heart tssue to the cardiac blood
cardiac blood may be significantly elevated which may be good for preliminary investigations
changes start in 1h and usually done in 24hr

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8
Q

more accurate blood level sites

A

femoral and cardiac may allow for more accurate interpretation of blood level at time of death

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9
Q

some drugs that undergo postmortem redistribution

A

TCA

digoxin

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10
Q

info to aid interpretation in living person

A

signs and symptoms
admission of drug or alcohol use
evidence found at scene

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11
Q

4 stages of tox exam

A
  1. screen - for as many drugs as possble with as little samples as possible
    confirm - 2 aliquots with different tests need to match
    quantification - need to use blood, liver, stomach
    interpret - toxic, therapeutic, lethal
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12
Q

improper specimen selection, collection, and submission =

A

inaccurate results

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13
Q

how to ensure continuity of exhibits

A
labelled by each possesser
sent by courier
adhesive tape to seal container
kept under lock and key
no access to lab to anyone unauthorized
transfer slips used
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14
Q

types of blood samples and what they contain

A

serum
plasma - clotting factors
whole blood - RBC need to detect CO poisoning

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15
Q

most reliable sample for alcohol and drug impairment

A

blood

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16
Q

what is sodium fluoride

A

preservative to prevent breakdown of some drugs

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17
Q

benefits of urine sample

A

non invasive
little to no sample prep
drugs/alcohol found in higher concentrations here
longer detection periods

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18
Q

what is meant by urine having a long detection period

A

drug concentration that is detectable will be in the urine much longer than in the blood

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19
Q

disadvantages of urine

A

may not reflect level of impairment at time of incident - not quantitative
some drugs break down in body so detection difficult
subject to tampering

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20
Q

problems with cananbis detection in urine impairment vs probation

A

if used cannabis will be present in urine for long time

not able to convict someone of impaired driving but valid for someone who breached probation

21
Q

urine sample uses

A

used for identification
cant determine when or how much drug was taken (can be used to determine BAC if period over which urine was collected is known)
if refuse to provide sample = guilty

22
Q

hair sample advantages

A

noninvasive
easy to collect (crown of head)
no special storage requirements
useful in determining long term drug use

23
Q

disadvantages of hair samples

A

not useful for alcohol detection
costly
can be affected by environmental contamination (smoking)
racial and hair color bias - drugs incorporates more into dark hair than light
not useful for acute

24
Q

saliva collection method

A

expectorate into container
place cotton ball in mouth until saturated
may stimulate by chewing paraffin, rubber band, glass marble

25
Q

advantages of oral fluid sample

A
noninvasive
easy to collect
no medical personnal required to sample
drug detection time similar to blood
only parent drug detected
26
Q

disadvantages of oral fluid sample

A

requires sensitive analytical techniques
limited data regarding interpretation of oral fluid levels
recent smoking or oral consumption may complicate interpretation

27
Q

advantages of sweat sample

A
noninvasice
easy to collect with a patch
patch impermeable to environmental contaminants
tamper proof - has unique id number
only parent drug detected
(drug moves from body into patch)
28
Q

disadvantages of sweat sample

A

costly
requires sensitive methods for detection
entire patch consumed in analysis so cant retest
limit info regarding incorporation of drugs in sweat and significance in findings - only really used for probation

29
Q

samples used postmortem

A
blood 
urine
liver
stomach contents
bile
vitreous humor
CSF
kidnye
brain
lung
hair and fingernails
bone
heart
adipose tissue
site of injection
30
Q

explain blood collection post mortem

A

20ml from femoral and 20ml from cardiac
half of each sample in sodium fluoride to avoid breakdown of drugs and prevent formation of ethanol from bacteria
always whol eblood
often hemolyzed (RBC burst)

31
Q

advantages of liver sample

A

most valuble

drug accumuates here up to 100x the amount in blood

32
Q

liver may be only appropriate sample in

A

fire victims
babies
decomposed bodys

33
Q

what info might you gather from stomach contents

A

drug OD by oral ingestion
may be able to identify undissolved tablets
measure of unabsorbed drug at time of death
corrosive agents if erosion of stomach lining
detects parent drug not metabolites

34
Q

burnt almond smell =

A

cyanide

35
Q

garlic smell -

A

pesticides

36
Q

use for bile sample

A

waste product so just for identification not quantification

major route of elimination for some drugs - opiates, methadne, benzo

37
Q

what is vitreous humor used to detect

A

for alcohol when blood isnt good

not used for drugs

38
Q

advantages of vitreous humor sample

A

sterile environment so more true concentration
no putrefaction
clean sample

39
Q

disadvantage of vitreous humor sample

A

limited quantitiy

40
Q

when is CSF used

A

for alcohol when available
less subject to putrefaction and contamination
clean sample

41
Q

dis of CSF

A

limited quantity

diffficult to collect - spinal tap

42
Q

use kidney sample when

A

metal poisoning suspected

43
Q

use lung sample when

A

inhalation of poison

44
Q

samples less likely to use unless there is not a lot left to sample or unknown cause

A
kidney
brain
lung tissue
hair and fingernails
bone
heart
adipose tissue
sites of injection
45
Q

when does putrefaction occur and how to prevent

A

right away

freeze them

46
Q

carbon monoxide putrefaction

A

products interfere with analysis bc same UV spectrum

47
Q

cocain putrefaction issues

A

enzymes in blood continue to break down cocaine so then converted to inactive metabolite

48
Q

alcohol putrefaction issues

A

bacteria produces alcohol

49
Q

cyanide putrefaction issues

A

bacteria produce cyanide post mortem