for midterm Flashcards
what colonizes plastic catheters
staphylococcus epidermis
what causes skin infections
staphylcoccus aureus
what bacteria species for large intestine
Bacteroides
what maintain low pH of vagina
lactobacillus
large skin lesions w bright red outer border and partially clear central area
early localized lyme disease
chills, fever, cardiovascular instability
bacteremia
stupor (near unconscious and stiff neck)
meninges/cerebrospinal fluid needs to be evaluated
gram stain what colors
+ –> purple
- –> pink/red
acid fast stain: acid fast bacilli what color
pink/red
india ink useful for what and how identified
crytococcus neofarmus in CSF
identified by large, transparent capsules that displace the ink
potassium hydroxide prep spares what
spares fungi
blood agar , sheep/horse what kind of media
enriched
for catalse test + or -
stephylococci
streptococci and enterococci
stephylococci –> +
steptococci and enterococci –> -
complement fixation solution will turn what color if negative
pink
range for pyschrophil, mesophil, thermophil, and which one for human pathogens
psychohrils (0-20 C)
mesophils (20-45 C)
Thermoohils (45-90 C)
most human pathogens aremesophils
human pathogen pH
7-7.4
ETC how much ATP/Mol glucose vs substrate level phosphorylation
38 vs 2
beta-lactams (penicillin and cephalosporins)
inhibition of cell wall synthesis
polymixins (neomycin and bacitarcin)
alteration of cytosolic membrane integrity
(displace mg and ca)
erythromycin
binds to 50S ribosomal subunit and inhibits peptidyl transferases
(inhibition of ribosomal protein synthesis)
clindamycin
binds to 50S ribosomal subunit and inhibits RNA-dependent protein synthesis
(inhibition of ribosomal protein synthesis)
tetracyclines
binds to 30S ribosomal subunit and inhibits binding of tRNA to mRNA
(inhibition of ribosomal protein synthesis)
metronidazole
reduction of 5 nitro group
suppression of nucleic acid syntehsis
quinolones
inhibit DNA supercoiling by DNA gyrase
suppression of nucleic acid synthesis
sulfonamides
blocks conversion of PABA to dihydrofolic acid
inhibition of folic acid synthesis
conjugation
cell to cell contact
transduction
phage vector
trasformation
naked DNA
Griffith mice experimentation was R virulent or S
R