fish and mouse

Question 1

what useful techniques can be used on mouse?

Answer 1

mutagenesis, targeted knockout by use of pluripotent stem cells

Question 2

how are zebrafish eggs fertilised?

Answer 2

externally

Question 3

what happens in zebrafish cls (SOX10) mutant?

Answer 3

have no swim bladder, ear defects, enteric neurons missing, pigment cells missing

Question 4

what do mutations in SOX10 in humans cause?

Answer 4

waardenburg syndrome type IV (hearing loss+pigment abnormalities)

Question 5

what is transgenic animal?

Answer 5

animal carrying a gene that has been incorperated into its genome using recombinant DNA rechnology

Question 6

what happens in zebra fish with alpha actin GFP and claudinbGFP?

Answer 6

alpha actin- labels all skeletal muscle

claudinb - labels lateral line primordium and developing kidney duct

Question 7

what is meant by mouse undergoing turning?

Answer 7

embryo is initially inside out (endoderm on outside)