First Aid Special Growth Medias

Question 1

Sabouraud agar is used to grow what?

Answer 1

Fungi

Question 2

Agar containing Vanco, Trimethoprim and Colistin is called what? What organism does it help isolate?

Answer 2

Thayer-Martin Agar; N. gonorrhoeae and N. meningitidis

Question 3

Chocolate Agar is useful for isolating…

Answer 3

H. influenzae

Question 4

Charcoal yeast extract agar is used for isolation of what? The agar also needs to be buffered with which two compounds?

Answer 4

Legionella; Charcoal yeast agar is buffered with Cysteine and Iron

Question 5

H. influenzae is grown on Chocolate agar that has been enhanced with what two compounds?

Answer 5

Factor V (NAD+) and Factor X (Hematin)

Question 6

Bordet-Gengou Agar is sued to grow what? What is the base of the media?

Answer 6

B. pertussis; Bordet-Gengou is a potato based agar

Question 7

E. coli turn a metallic green on what agar?

Answer 7

Eosin-methylene blue agar (EMB)

Question 8

B. pertussi can be grown in media with Charcoal, blood and antibiotics. What is the name of the agar?

Answer 8

Regan-Lowe medium

Question 9

Lactose fermenters turn MacConkey Agar what color? Why?

Answer 9

They produce acid that turns the agar pink

Question 10

Tellurite agar and Lofflet medium are used to isolate what?

Answer 10

C. diphtheriae

Question 11

Eaton agar, with added cholesterol, allows isolation of what?

Answer 11

M. pneumoniae

Question 12

What agar would you use to grow up the bacteria sampled from a Ghon complex?

Answer 12

Lowenstein-Jensen Agar; used to culture TB