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IMSE LABORATORY > finals - viral > Flashcards

finals - viral Flashcards

1
Q

is a collection of virus coming from different families that has 1 similarities

A

hepatitis

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2
Q

size of a hepa B vius

A

42 nm enveloped virion

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3
Q

The envelope contains a protein called the _____, which is important to the laboratory diagnosis and
immunization.

A

surface antigen (HBsAg)

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4
Q

____ is the earliest serological marker that
indicate the presence of acute infection. It is also indicative of chronic infection.

A

HbsAg

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5
Q

incubation period of HIV

A

60 - 90 days

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6
Q

*The first generation tests were found on ____

A

Ouchterlony agar gel plates

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7
Q

positive result of first generational test

A

white precipitin line formed between the antigen and the antibody

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8
Q

The test was said to be not very sensitive

A

The first generation tests

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9
Q

the main similarities of hepatitis is infects the ___

A

liver

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10
Q

the hepatitis virus that has dna as its genetic material

A

hepatitis B

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11
Q

fam of hepatitis B

A

hepaDNAviridae

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12
Q

first hepatitis to be discovered

A

hepatitis B

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13
Q

who discovered Hepa B

A

Baruch Blumberg 1963

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14
Q

MOT

A

parenteral, sexual contact, perinatal (congenital)

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15
Q

hepa B is known for causing

A

liver cirrhosis
chronic liver disease

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16
Q

hepa B is known as

A

serum hepatitis

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17
Q

how to diagnose hepatitis

A

serologic (Ag and Ab)
and
molecular (confirmatory)

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18
Q

___ test determines genetic material of the virus

A

molecular

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19
Q

hepatitis B serologic markers

antigens

A

Surface - HBsAg
Core - HBcAg
envelope - HBeAg

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20
Q

hepatitis B serologic markers

antibodies

A

Anti - Hbs
Anti Hbc
Anti Hbe

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21
Q

surface marker that will determine early acute infection/chronic infection of hepatitis B

A

surface - HBsAg

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22
Q

a surface marker that can only be found in hepatocytes

A

core - HBcAg

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23
Q

surface markers - antigens - that can be found in serum

A

HBsAg
HBeAg

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24
Q

presence of antigen HBcAg means that the patient is ___

A

highly infectious

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25
Q

a serologic marker for immunity to hepatitis
– natural immunity
– vaccinated

A

Anti - HBs

26
Q

a serologic marker that will signifies past and present infection

A

Anti HBc

–IgM
–IgG

27
Q

A presence of IgM upon confirming the presence of Anti-HbC signifies

A

Present infection

28
Q

A presence of IgG upon confirming the presence of Anti-HbC signifies

A

Past infection

29
Q

a serologic marker for convalescent phase which means that the patient is already in recovery period

A

Anti HBe

30
Q

agent of dengue

A

dengue virus

31
Q

vector for dengue

A

aedes aegypti
aedes albopictus

32
Q

where can we found the
aedes aegypti
aedes albopictus

A

mosquitoes in tropical areas

33
Q

2 serological test for dengue

A

NS1 for antigen
and
IgG and IgM for antibody

34
Q

serological test - ____ - is an immediate marker if you only got it for the first time

A

NS1

35
Q

family of HIV

A

Retroviridae and subfamily ____

36
Q

2 types of hiv

A

hiv 1
hiv 2

37
Q

considered as a pandemic HIV

A

HIV 1

38
Q

which type of HIV is more pathogenic

A

HIV 1

39
Q

where can we find HIV 2?

A

only in africa// recent reports states that the virus mutates and has a strain outside Africa 2

40
Q

MOT of transmission of HIV

A

perinatal, sexual contact, blood transfusion (blood, breast milk, vaginal fluid, csf, spinal fluid, pleural fluid)

41
Q

stages of HIV

A
  1. acute hiv infection
  2. clinical latency
  3. AIDS
42
Q

how long will acute hiv infection takes place

A

2-4 weeks

43
Q

in acute hiv infection, there will be a large amount o f___

A

viral

44
Q

clinical latency stage of hiv will take how many yrs

A

period can last more than 10 years or decade

45
Q

in the end of clinical latency stage of hiv, what will increase and decrease?

A

viral increases
CD 4 cells decreases

46
Q

last stage of HIV

A

AIDS

47
Q

cd4 cells in AIDS is around

A

will start <200/mm3 to <40/mm3 (severe)

48
Q

opportunistic pathogens will cause disease in this stage of HIV

A

AIDS

49
Q

what is the most common opportunistic disease in AIDS

A

tuberculosis

50
Q

screening test for HIV

A

1st gen to 5th gen

51
Q

which generational test for HIV will use purified lysate as antigens

A

first gen

52
Q

which generational test for HIV will use recombinant viral proteins

A

2nd gen

53
Q

The ____ test used for HbsAg was counter
electrophoresis. T

A

second-generation

54
Q

in 2nd generational test, The plates were subjected to electric current. If the
antigen is on the serum, a ____ would form.

A

fine white precipitin line

55
Q

which generational test for HIV will use double antigen sandwich assay

A

3rd gen

56
Q

which generational test for HIV will detect antibody and p24 antigen

A

fourth gen

57
Q

which generational test for HIV will use multiplex assay

A

fifth gen

58
Q

positive in HBsAg
negative in anti HBs
negative in anti HBc

A

early infection

59
Q

positive in HbsAg
negative in Anti Hbs
positive in anti-Hbc

A

chronic infection

60
Q

negative in HbsAg
negative in Anti-Hbs
Positive in AntiHbc

A

window period

61
Q

negative in HbsAg
positive in anti-Hbs
positive in anti-Hbc

A

recovery

62
Q

negative in HbsAg
positive in Anti-Hbs
negative in Anti-Hbc

A

vaccination

IMSE LABORATORY (8 decks)

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