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febrile antigens Flashcards

1
Q

materials used for widal test for febrile antigens

A

*Antigen suspension S. typhi O
Antigen suspension S. typhi ‘BH’
*Antigen suspension S. typhi H
Antigen Suspension S. typhi ‘AH’
*Pipettes
Test tubes Antigen Vortex mixer
*Glass slides
Mixing sticks
*NSS

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2
Q

an infection or fever caused by salmonella spp

A

typhoid fever or typhoid salmonella

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3
Q

___ infection is caused by Rickettsia spp

A

ricketsiall infection

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4
Q

___ are reagent containing bacterial antigens

A

febrile antigens

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5
Q

*Typhoid fever, simply typhoid, caused by the bacterium ____

A

Salmonella enterica
subsp. enterica serovar Typhi

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6
Q

Salmonella enterica
subsp. enterica serovar Typhi grows in what part of the body?

A

intestine and blood

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7
Q

____ is a cook poor hygiene - fecal contaminants mixed with food

A

mary mallon

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8
Q

Typhoid is spread by ___

A

eating or drinking food or water contaminated with the
FECES of an infected person.

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9
Q

TSI tube result of salmonella spp

A

alk/acid - red/yellow butt with black colored (hydrogen sulfide)

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10
Q

*Diagnosis is made by any ___ and with
the Widal test (demonstration of antibodies against Salmonella antigens
O-somatic and H-flagellar).

A

blood, bone marrow, or stool cultures

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11
Q

a therapeutic trial time with ___ is generally undertaken while awaiting the results of the Widal test and cultures
of the blood and stool.

A

chloramphenicol

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12
Q

what are the methods used for diagnosing salmonella infection

A

1.) Culture (bacteria) -
2.) Kauffman and White classification scheme - 3.) Widal test
4. Typhidot -

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13
Q

a method of diagnosing salmonella infection which is the gold standard

A

culturing bacteria

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14
Q

a method of diagnosing salmonella infection which focused on classifying sero type

A

2.) Kauffman and White classification scheme

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15
Q

a method of diagnosing salmonella infection in which it looks for the IgG and IgM

A

typhidot

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16
Q

In Typhidot, a detection of IgG indicates the presence of what infection?

A

past infection

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17
Q

In Typhidot, a detection of IgM indicates the presence of what infection?

A

present infection

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18
Q

a method of diagnosing salmonella infection in which it Quantitatively measures the antibodies in the serum of patient with Typhoid fever

A

WIDAL TEST

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19
Q

3 kinds of antigen being detected in widal test

A

O antigen
H antigen
K antigen

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20
Q

also known as Somatic antigen

A

O antigen

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21
Q

an antigen that is heat stable

A

O antigen

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22
Q

it is an antigen that is present in the cell wall of the bacteria

A

O antigen

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23
Q

an antigen that is also known as the flagellar antigen

A

H antigen

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24
Q

Is H antigen or flagellar antigen heat stable?

A

nope

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25
it is an antigen that is found in the flagella
H antigen or flagellar antigen
26
an antigen used for widal test
commonly is the O antigen, but sometimes with H antigen
27
the antigen that is a virulence factor of salmonella
K antigen
28
K antigen is also known as
Vi antigen
29
K antigen is the promoter of the ___ of the salmonella
capsulation
30
this antigen is resistant to phagocytosis
K antigen
31
the antigen that is used by the reference laboratories
K antigen
32
3 Types of Methods in WIDAL’s:
Rapid Slide Screening Method Rapid slide titration method Tube agglutination test
33
a *1/+ agglutiation means
Less than 25% of the organism is agglutinated
34
*1+ agglutination means
25% of the organism is agglutinated
35
2+ agglutination means
50% of the organism is agglutinated
36
*3+ agglutination means
75% of the organism is agglutinated
37
100% of the organism is agglutinated
4+
38
Rapid Slide Titration Method give the corresponding ml used each circle
0.08ml, 0.04ml, 0.02ml, 0.01 and 0.005ml
39
Serum Volume 0.08 0.04 0.02 0.01 0.005 give the corressponding tube dilution
1:20 1:40 1:80 1:160 1:320
40
incubation time for salmonella O antigens and proteus
50*C for 4 hours
41
incubation time for salmonella H antigens
50*C for 2 hours
42
It is necessary to perform all dilutions in the slide test to obviate the “prozone” effect where higher concentrations of the serum may give a negative result but further dilutions may give a positive result true or false
true
43
A single negative test does not exclude the absence of infection true or false
true
44
A single positive test is of no diagnostic value unless the titer is sufficiently low. true or false
sufficiently high
45
The considered significant titer in the Widal test is ___ while ____ in the Weil-Felix Test
1:80; 1:160
46
Two Types of Agglutination Test in WIDAL’S
somatic agglutination flagellar agglutination
47
Compact aggregates that are not easily dispersed Stronger agglutination than H Ag
Somatic Agglutination
48
Fluffy and floccular aggregates that are easily dispersed
Flagellar Agglutination
49
Significant titer for widal
> 80
50
INC titer on OD Ag:
indicates acute/ recent typhoid fever
51
INC titer on OA, OB and OC:
indicates acute/ recent paratyphoid infection
52
INC titer on HD Ag
indicates previous/ past infection with typhoid fever *-Convalescent/ vaccination against typhoid fever
53
INC titer on HA, HB, HC
indicates past infection on S.paratyphi
54
INC titer on O and H Ag:
: indicates mixed infection
55
*NON SPECIFIC TEST
WEIL- FELIX TEST
56
Used to test for antibodies to rickettsia infections of Typhus fever caused by: (R. prowazekii)
WEIL- FELIX TEST
57
Proteus ox-2 is from
Proteus vulgaris
58
proteus ox 19 is from
Proteus vulgaris
59
Proteus ox-k uses
Proteus mirabilis
60
*Endemic Typhus:
May agglutinate “OX-19 and OX-2”
61
Tick Borne Spotted Fever
May agglutinate “OX-19 and OX-2”
62
Scrub Typhus
OX-K strain”
63
TEST IS NEGATIVE IN
*RICKETSSIAL POX *TRENCH FEVER *Q-FEVER
64
___ reaction may occur in urinary or other Proteus infection. Test maybe negative in 50 percent scrub typhus
False Positive
65
rocky maintain spotted fever
ox19 and ox 2
66
brill zinsser disease
ox19 +-1
67

IMSE LABORATORY (8 decks)

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