febrile antigens Flashcards

1
Q

materials used for widal test for febrile antigens

A

*Antigen suspension S. typhi O
Antigen suspension S. typhi ‘BH’
*Antigen suspension S. typhi H
Antigen Suspension S. typhi ‘AH’
*Pipettes
Test tubes Antigen Vortex mixer
*Glass slides
Mixing sticks
*NSS

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2
Q

an infection or fever caused by salmonella spp

A

typhoid fever or typhoid salmonella

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3
Q

___ infection is caused by Rickettsia spp

A

ricketsiall infection

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4
Q

___ are reagent containing bacterial antigens

A

febrile antigens

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5
Q

*Typhoid fever, simply typhoid, caused by the bacterium ____

A

Salmonella enterica
subsp. enterica serovar Typhi

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6
Q

Salmonella enterica
subsp. enterica serovar Typhi grows in what part of the body?

A

intestine and blood

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7
Q

____ is a cook poor hygiene - fecal contaminants mixed with food

A

mary mallon

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8
Q

Typhoid is spread by ___

A

eating or drinking food or water contaminated with the
FECES of an infected person.

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9
Q

TSI tube result of salmonella spp

A

alk/acid - red/yellow butt with black colored (hydrogen sulfide)

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10
Q

*Diagnosis is made by any ___ and with
the Widal test (demonstration of antibodies against Salmonella antigens
O-somatic and H-flagellar).

A

blood, bone marrow, or stool cultures

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11
Q

a therapeutic trial time with ___ is generally undertaken while awaiting the results of the Widal test and cultures
of the blood and stool.

A

chloramphenicol

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12
Q

what are the methods used for diagnosing salmonella infection

A

1.) Culture (bacteria) -
2.) Kauffman and White classification scheme - 3.) Widal test
4. Typhidot -

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13
Q

a method of diagnosing salmonella infection which is the gold standard

A

culturing bacteria

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14
Q

a method of diagnosing salmonella infection which focused on classifying sero type

A

2.) Kauffman and White classification scheme

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15
Q

a method of diagnosing salmonella infection in which it looks for the IgG and IgM

A

typhidot

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16
Q

In Typhidot, a detection of IgG indicates the presence of what infection?

A

past infection

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17
Q

In Typhidot, a detection of IgM indicates the presence of what infection?

A

present infection

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18
Q

a method of diagnosing salmonella infection in which it Quantitatively measures the antibodies in the serum of patient with Typhoid fever

A

WIDAL TEST

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19
Q

3 kinds of antigen being detected in widal test

A

O antigen
H antigen
K antigen

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20
Q

also known as Somatic antigen

A

O antigen

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21
Q

an antigen that is heat stable

A

O antigen

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22
Q

it is an antigen that is present in the cell wall of the bacteria

A

O antigen

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23
Q

an antigen that is also known as the flagellar antigen

A

H antigen

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24
Q

Is H antigen or flagellar antigen heat stable?

A

nope

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25
Q

it is an antigen that is found in the flagella

A

H antigen or flagellar antigen

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26
Q

an antigen used for widal test

A

commonly is the O antigen, but sometimes with H antigen

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27
Q

the antigen that is a virulence factor of salmonella

A

K antigen

28
Q

K antigen is also known as

A

Vi antigen

29
Q

K antigen is the promoter of the ___ of the salmonella

A

capsulation

30
Q

this antigen is resistant to phagocytosis

A

K antigen

31
Q

the antigen that is used by the reference laboratories

A

K antigen

32
Q

3 Types of Methods in WIDAL’s:

A

Rapid Slide Screening Method
Rapid slide titration method
Tube agglutination test

33
Q

a *1/+ agglutiation means

A

Less than 25% of the organism is
agglutinated

34
Q

*1+ agglutination means

A

25% of the organism is agglutinated

35
Q

2+ agglutination means

A

50% of the organism is agglutinated

36
Q

*3+ agglutination means

A

75% of the organism is agglutinated

37
Q

100% of the organism is agglutinated

A

4+

38
Q

Rapid Slide Titration Method

give the corresponding ml used each circle

A

0.08ml, 0.04ml, 0.02ml, 0.01 and 0.005ml

39
Q

Serum Volume

0.08
0.04
0.02
0.01
0.005

give the corressponding tube dilution

A

1:20
1:40
1:80
1:160
1:320

40
Q

incubation time for salmonella O antigens and proteus

A

50*C for 4 hours

41
Q

incubation time for salmonella H antigens

A

50*C for 2 hours

42
Q

It is necessary to perform all dilutions in the slide test to obviate the “prozone” effect where
higher concentrations of the serum may give a negative result but further dilutions may give a
positive result

true or false

A

true

43
Q

A single negative test does not exclude the absence of infection

true or false

A

true

44
Q

A single positive test is of no diagnostic value unless the titer
is sufficiently low.

true or false

A

sufficiently high

45
Q

The considered significant titer in the Widal test is ___ while
____ in the Weil-Felix Test

A

1:80; 1:160

46
Q

Two Types of Agglutination Test in WIDAL’S

A

somatic agglutination
flagellar agglutination

47
Q

Compact aggregates that are not easily dispersed

Stronger agglutination than H Ag

A

Somatic Agglutination

48
Q

Fluffy and floccular aggregates that are easily
dispersed

A

Flagellar Agglutination

49
Q

Significant titer for widal

A

> 80

50
Q

INC titer on OD Ag:

A

indicates acute/ recent typhoid fever

51
Q

INC titer on OA, OB and OC:

A

indicates acute/ recent
paratyphoid infection

52
Q

INC titer on HD Ag

A

indicates previous/ past infection with
typhoid fever
*-Convalescent/ vaccination against typhoid fever

53
Q

INC titer on HA, HB, HC

A

indicates past infection on
S.paratyphi

54
Q

INC titer on O and H Ag:

A

: indicates mixed infection

55
Q

*NON SPECIFIC TEST

A

WEIL- FELIX TEST

56
Q

Used to test for antibodies to rickettsia infections of
Typhus fever caused by: (R. prowazekii)

A

WEIL- FELIX TEST

57
Q

Proteus ox-2 is from

A

Proteus vulgaris

58
Q

proteus ox 19 is from

A

Proteus vulgaris

59
Q

Proteus ox-k uses

A

Proteus mirabilis

60
Q

*Endemic Typhus:

A

May agglutinate “OX-19 and OX-2”

61
Q

Tick Borne Spotted Fever

A

May agglutinate “OX-19 and OX-2”

62
Q

Scrub Typhus

A

OX-K strain”

63
Q

TEST IS NEGATIVE IN

A

*RICKETSSIAL POX
*TRENCH FEVER
*Q-FEVER

64
Q

___ reaction may occur in urinary or other
Proteus infection. Test maybe negative in 50 percent
scrub typhus

A

False Positive

65
Q

rocky maintain spotted fever

A

ox19 and ox 2

66
Q

brill zinsser disease

A

ox19 +-1

67
Q
A