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IMSE LABORATORY > ASO LATEX AGGLUTINATION TEST > Flashcards

ASO LATEX AGGLUTINATION TEST Flashcards

1
Q

what bacteria is the one we test for ASO latex agglutination test

A

streptococcus pyogenes

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2
Q

in terms of lancefield group, what group do streptococcus pyogenes do belong?

A

group A

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3
Q

streptococci are divided into how many groups?

A

20

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4
Q

what is the hemolytic pattern of group A streptococcus

A

alpha - partial hemolysis and a greenish discoloration

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5
Q

what is the main virulence factor of streptococcus pyogenes?

A

m protein

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6
Q

the virulence factor of streptococcus pyogenes - M protein functions as

A

as inhibitor of phagocytosis

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7
Q

what are the exo enzymes of streptococcus

A

streptolysin O
streptokinase
hyaluronidase
DNAse B
NADase

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8
Q

among the exoenzyme of streptococcus pyogenes, which one is the most immunogenic(capable of inducing immune reactions) - can cause antibody reaction

A

streptolysin O

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9
Q

among the exoenzyme of streptococcus pyogenes, which one is the oxygen stable and not capable of inducing immune reactions) -

A

streptokinase

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10
Q

what is the general rule of ASO latex agglutination test?

A

always use 2 antibodies

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11
Q

the exo enzyme of streptococcus pyogenes that it lyses the rbc and also known as oxygen labile

A

streptolysin O

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12
Q

the exo enzyme of streptococcus pyogenes that inhibits clotting - mechanism to isolate infection

A

streptokinase

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13
Q

the exo enzyme of streptococcus pyogenes that is the spreading factor

A

hyaluronidase

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14
Q

the exo enzyme of streptococcus pyogenes that destroys dna

A

DNAse B

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15
Q

the exo enzyme of streptococcus pyogenes that destroys NAD

A

NADase

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16
Q

what are the ways of streptococcus pyogenes to counter attack

A

–avoid wbc
–inactivate complementary system
–inhibits phagocytosis - using protein and capsule to inactivate it

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17
Q

the presence of polysaccharide capsules are usually seen in __ they can inhibit phagocytosis using capsule that evolves from their cell body

A

Neisseria meningitidis, streptococcus pneumoniae, yersinia pestis, haemophilus influenzae

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18
Q

hall marks or the signs for streptococcus pyogenes

A

upper respiratory tract infection (pharyngitis and tonsilitis)

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19
Q

skin infections caused by streptococcus pyogenes

A

skin infection (skin impetigo)
streptococcal pyoderma
Erysipelas- form of skin infection
cellulitis - inflammation of folicles
toxic shock syndrome

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20
Q

inflammation of follicles

A

cellulitis

21
Q

gastrointestinal tract infection

A

puerperal sepsis

22
Q
A
23
Q

___ - characterzied by rashes. Second disease causing rash

A

SCARLET FEVER

24
Q

inflammation of the middle ear

A

otitis media

25
Q

first disease causing rash

A

measles

26
Q

cardinal signs of streptococcus pyogenes- caused by pyrogenic exotoxins of S. pyogenes

A

strawberry colored tongue

27
Q

a further consequences of having strepto (aftermath)

A

damaging sequale

28
Q

2 damaging sequale

A
  1. acute rheumatic fever
  2. post streptococcal glomerulonephritis
29
Q

the antibodies will attach the antigens in the heart and kidney that is similar to the antigen for strepto
- called ___

A

cross reaction/reactivity

30
Q

IN ORDER TO DETECT ANTIGENS WE USED THE what assay?

A

LATERAL FLOW IMMUNOCHROMATOGRAPHY ASSAY

31
Q

what is the principle of aso slide method or the aso latex agglutination test

A

Passive/ Indirect Agglutination

32
Q

bacterial screening for screening is the

A

BACITRACIN TEST - susceptible in bacitracin test

33
Q

confirmatory test for S. pyogenes

A

positive in PYR - Pyrrolidonyl arylamidase

34
Q

detection of antibodies is LONG terms as it can detect the PAST infection as well

true or false

A

true

35
Q

ASO LATEX REAGENT

A

Suspension of
polystyrene particles sensitized with
Streptolysin O

36
Q

Analytical Sensitivity

A

200 IU/mL

37
Q

positive for aso latex agglutination test

A

if titer is > 200 IU/ml

38
Q

3 reagents used for aso slide method

A

ASO LATEX REAGENTS
ASO POSITIVE CONTROL
ASO NEGATIVE CONTROL

39
Q

all reagents contain ___ as a preservative

A

0.1% sodium azide

40
Q

ASO SLIDE METHOD

A
  1. Allow each components of the test kit (reagents, controls) to reach room temperature.
  2. Gently shake the latex reagent to disperse the particles.
  3. Place a drop of undiluted serum into a circle of a test slide.
  4. Add one drop of ASO Latex reagent next to the drop of
    serum.
  5. Spread the reagent and serum sample over the entire area of
    the test circle using a separate stirrer for each sample.
  6. Gently tilt the test slide backwards and forwards for two minutes.
  7. Observe and interpret the results.
41
Q

Diagnosis of
the probability for
sequelae
S. pyogenes infection

A

ASO TUBE TEST

42
Q

type of dilution for aso tube test

A

Compound dilution

43
Q

Principle for aso tube test

A

NEUTRALIZATION/Enzyme
inhibition test

44
Q

Multi-Enzyme test to detect
Antibody to the multiple
exoenzymes of S. pyogenes

A

Streptozyme Test

45
Q

PRINCIPLE of streptozyme test

A

Passive Hemagglutination

46
Q

reagents used for streptozyme test

A

Formalinized/ Aldehyde fixed
sheep RBCs coated with exoenzymes of S.
pyogenes

47
Q

how many tubes are used for aso tube test

A

14 tubes

48
Q

what is the titer for aso tubes test

A

Titer: last tube with no hemolysis

49
Q
A

IMSE LABORATORY (8 decks)

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