Fate Maps Flashcards
What is clonal analysis
study of progeny of a single cell
What does clonal analysis show
what fates a cell can give rise to
Which type of commitment is reversible
specification
Describe determination
irreversible cell commitment
Describe mosaic development
Cells are determined early, isolated parts develop as normal
How does an embryo develop mosaically if split in two
half a normal embryo develops - not normally patterned
Describe regulative development
Cells are NOT determined early
How does an embryo develop regulatively if split in two
normally patterned twin
Describe autonomous development
intrinsic factors make cells different from one another
Describe non-autonomous mechanisms
extrinsic signals make cells different
What are the key features of making a fate map
observation in embryos, natural markers, application of label
What does a fate map discover
what cells normally become
Describe an orthotopic transplantation
transplant cells from one embryo to another embryo
What are the properties of an ideal marker
readily visible at later stages, inherited by ALL progeny, X perturb development, X leak
What are the useful markers for fate mapping
vital dyes, high molecular tracers, cytological labels
