Factors Affecting Enzyme Activity

Question 1

How does temp affect enzyme activity

Answer 1

As temp increase gives more kinetic energy so the molecules move faster so enzyme more likely to collide with substrate. If the temp goes above a certain point vibration breaks some of bonds that hold enzymes in shape. Active site changes am and enzyme and substrate no longer fit together. So denatured

Question 2

How does Ph affect enzyme activity?

Answer 2

All enzymes have optimum ph value
Pepsin ph 2
H+ and oh- ions can break ionic and hydrogen bonds which changes the tertiary structure and denatures the enzyme

Question 3

How does enzyme concentration affect rate of reaction

Answer 3

More enzyme molecules more likely to collide with a substrate thereofore increase reaction
If amount of substrate is limited no further affect st a point

Question 4

How does substrate concentration affect rate of reaction

Answer 4

More likely to collide with enzyme

Question 5

How does a competitive inhibitor decrease enzyme activity?

Answer 5

Have similar shape to that of substrate molecules
Bind to active sight no reaction
Block active site do they can’t fit in
How much inhibited depends on concentration of inhibitor

Question 6

How does a non competitive inhibitor affect enzyme activity?

Answer 6

Binds away from active site
Causes active site to change shape so substrate can no longer bind
Increasing conc of substrate won’t make any difference to reaction rate

Question 7

What are the 5 factors affecting enzyme activity?

Answer 7

Temp
Ph
Enzyme conc
Substrate conc
Enzyme inhibitors