facilty design/biosecurity

Question 1

biosecurity goals

Answer 1

1. eliminate targeted diseases, reduce risk of introduction of new disease
2. reduce spread of endemic disease/subclinical disease
3. optimize welfare
4. optimize uniformity of animals

Question 2

healthy animal “units”:

Answer 2

minimize std deviation in research

Question 3

how does uniformity contribute to prudent animal use?

Answer 3

minimize sample size, more sensitive detection of differences
refinement-> reduced pain/stress
reduces loss of genetics, reduced cost

Question 4

why are more susceptible animals being used

Answer 4

reduced pathogen load, reduced immune competence (transgenics), new phenotypes

Question 5

biosecurity vs biosafety

Answer 5

biosecurity- measures to control infection
containment- manage infectious materials
exclusion- keep pathogens out

biosafety- reduce risk for workers

Question 6

primary vs secondary biocontainment

Answer 6

primary: specialized caging, animal manipulations within cage, PPE

secondary: ventilation, room construction, SOPs, decontamination, facility layout, limited personnel access

Question 7

bioexclusion practices

Answer 7

get animals from SPF suppliers, pest control, use clean materials, restrict access

Question 8

facility layout considerations

Answer 8

differential pressures, dirty and clean areas separately, durable for sanitation, housing rooms

Question 9

animal facilites at ucalgary

Answer 9

foothills- germ free, SPF, conventional, transgenic, optogenetics, quarantine, level 2 and 3
main campus- std, level 2, rederivation
spyhill- wild mice, lg animal, wildlife

Question 10

biosafety hierarchy of controls

Answer 10

Question 11

what factors to consider for new animals entering facility?

Answer 11

• health status
• dx tests in the last 3m
• hx of any +ves at the facility
• health program/husbandry

Question 12

animal health status from different sources

Answer 12

commercial- SPF, confirmed health/genetics
non commercial- sideline, variable background

Question 13

vet role in importation?

Answer 13

• identify potential risks of supplier
• establish list of excluded pathogens
• determine risk, tell stakeholders

Question 14

quarantine process

Answer 14

containment/exclusion for 6 wks: additional testing, ectoparasite testing, serology/pcr, sentinel necropsy

Question 15

mouse rederivation facility

Answer 15

primary containment w negative pressure bubble
SOPs- PPE, limited access, entered last

Question 16

outbreak control options

Answer 16

1. confirm +ve
   move positive racks into new rooms for isolation and tx

negative racks can stay, start microisolation

complete room change out, environmental sampling