Explain the major limitation of murine monoclonal antibody

Question 1

Murine monoclonal antibody

Answer 1

Antibodies created in rats from identical b cells which are clones from a single parent cell.
First produced in 1975

Question 2

Limitation

Answer 2

Due to the murine origins of the antibodies they were immunogenic in humans and were poor inducers of immunity in patients

Question 3

Alternative

Answer 3

Alternative to murine monoclonal antibodies would be chimeric or humanised monoclonal antibodies as their protein sequence are made more similar to human antibodies

Question 4

Chimeric antibodies

Answer 4

Antibody made up of molecules from domains from different species

Question 5

Humanised antibody

Answer 5

The conversion of a non-human monoclonal antibody to be as humanlike as possible with respect to immunogenicity, but without losing the antigen specificity and binding affinity of the original antibody