Exercise 15 - effects of oxygen on growth

Question 0

Ingredients in the medium that acts to lower the O/R

Potential (oxygen amounts)

Answer 0

Glucose

Sodium thioglycollate

Cysteine

Question 1

Name of the medium we used to determine oxygen requirements

Answer 1

Fluid thioglycollate media - FTM

Question 2

What is the meaning of the pink (red) color at the top of the tube

Answer 2

Presence of oxygen

Question 3

Name of the indicator dye

What does it detect

Answer 3

Resazurin

Oxygen

Question 4

Why is the medium boiled before inoculation

Answer 4

To remove the oxygen from the FTM

Question 5

Obligate aerobic

Answer 5

Will be at the top of the FTM

Question 6

Microaerophillic

Answer 6

Will be suspended somewhere toward the middle

Question 7

Facultative anaerobes

Answer 7

Will be noticed throughout the entire tube

Question 8

Obligate anaerobes

Answer 8

Will be at the bottom of the tube

Question 9

What are the two enzymes obligate anaerobes don’t have

Answer 9

Superoxide dismutase

Catalase

Question 10

FTM contains what 4 ingredients

Answer 10

Glucose - fuel

Sodium thioglycollate
Cysteine

Resazurin

Question 11

What three bacteria were used in our FTM tubes

Answer 11

Pseudomonas aeruginosa

Clostridium sporogenes

E. Coli

Question 12

What is Clostridium sporogenes

Answer 12

Obligate anaerobe

Question 13

What is E. Coli

Answer 13

Facultative anaerobe

Question 14

What is Pseudomonas aeruginosa

Answer 14

Obligate aerobe