Excersize 2 - Aseptic Technique

Question 0

Flaming loops and needles

Answer 0

Flame before and after transferring cultures

Question 1

How to label and incubate petri plates?

Answer 1

(Clockwise from top right of dish)

Sample name
Initials
Group (table #)
Date
Incubation time
Incubation temperature = 37 degrees Celsius (24-48hrs)

Question 2

Flaming mouth of culture tubes

Answer 2

Flame when cap first comes off, and before it goes on

Question 3

Transferring organisms from broth to broth

Be able to correctly order all steps

Answer 3

Step 1: flame loop
Step 2: Shake tube
Step 3: remove cap, flame mouth
Step 4: loop in tube
Step 5: remove loop, flame mouth
Step 6: cap tube

Repeat with sterile tube, but don’t flame loop in beginning

Question 4

Transferring organisms from slant to slant

Answer 4

1. Flame loop
2. Remove cap, flame mouth
3. Loop in tube
4. Loop out, flame mouth
5. Recap and return
6. Uncap sterile tube, flame mouth
7. Loop in tube
8. Flame mouth, recap
9. Flame loop, return loop

Question 5

Transferring from broth to slant?

Answer 5

Same as broth to broth

Question 6

Transferring organisms from a petri plate to a slant or broth

Answer 6

1. Flame loop
2. Open petri, loop in
3. Flame mouth of sterile tube, loop in
4. Flame mouth, recap
5. Flame loop