Excersize 8 - Negative Staining

Question 0

What does the organism and background look like on a negative stain

Answer 0

Organism is clear

Dark background

Question 1

Procedure for negative staining

Answer 1

Organisms are dispersed in a small drop of nigrosin

Use another slide to spread the drop backwards

Question 2

Benefits of negative staining

Answer 2

good for accurately determining cell size (because of no heat fixing)

good for viewing capsule around cell

good for viewing spirochaetes