Excersize 3 - Brightfield Microscopy

Question 0

Calculation of total magnification

Answer 0

Ocular x Objective

Question 1

Parts of the microscope

Answer 1

See microscope worksheet

Question 2

Relationship between magnification and working distance

Answer 2

The greater the magnification the smaller the working distance

Question 3

Focusing procedure

Answer 3

Start at 10x, then 20x, then 40x, then finally 100x. Focusing and centering specimen along the way

Question 4

What type of specimens can be viewed: stained or unstained? Live or dead?

Answer 4

Live cells unstained

Dead cells stained

Question 5

What does parfocal mean? What does it allow you to do??

Answer 5

Means image will remain in focus when changing low power objective to high power objective.
Just minimally adjust focus

Question 6

How to properly handle and carry a microscope

Answer 6

Use both hands, one on the arm and one on the base

Question 7

How to clean it and return storage ready

Answer 7

Use cleaning tissue and solvents, use storing steps.