Exercise 14 - Motility Determination Flashcards
0
Q
Advantages of wet mounts
A
Fast result
1
Q
Procedure for wet mount
A
Put two loopfuls of bacteria in slide
Cover quickly
Look using dark field adapter
2
Q
Disadvantages of wet mounts
A
Dangerous if spill
Dry up fast
Brownian motion
3
Q
Procedure for soft agar stab
A
Put bacteria on needle
Stab the Motility Medium tube almost all the way down (straight down)
4
Q
Advantages of motility medium
A
Safer
Easier to see
5
Q
Disadvantages of motility medium
A
Require incubation time
6
Q
What were the bacteria used in this exercise
A
Proteus mirabilis
Staphylococcus aureus
7
Q
Properties of MTM (motility medium)
A
Contains .4% agar (softer than normal) and TTC
Bacteria react with TTC to form Formazan (red participate)
8
Q
Which bacteria was motile
A
Proteus mirabilis
9
Q
Which bacteria was not motile
A
Staphylococcus aureus
