Exam 2 Wiley Plus Quizes

Question 1

Translation initiation depends on the ribosome binding to a specific region of the mRNA known as the

Answer 1

Shrine-Dalgarno sequence

Question 2

DNA replication in bacteria is initiated at the

Answer 2

Ori site

Question 3

When compared to a template strand of DNA, the corresponding RNA strand is

Answer 3

complementary

Question 4

What is the correct sequence of protein action during DNA replication?

1. DNA polymerase
2. Single stranded binding proteins
3. DNA ligase
4. Helicase
5. Primase

Answer 4

4-5-2-1-3

Question 5

Hershey and Chase used 35S and 32P to distinguish between proteins and nucleic acids. Why not just use 15N?

Answer 5

Because nitrogen is found in both proteins and nucleic acids, so there would be no way to distinguish between them

Question 6

Insertions and deletions are frameshift mutations T/F

Answer 6

true

Question 7

When Hershey and Chase determined that a bacterial virus labeled with 35S did not enter the bacterium and give rise to a new generation of viral particles, they were able to eliminate _____ as the genetic material.

Answer 7

proteins

Question 8

a missense mutation

Answer 8

results in an amino acid codon converting to a codon for a different amino acid

Question 9

When the genetic code is described as redundant, this means

Answer 9

most amino acids are coded for by more than one codon.

Question 10

In the language of nucleic acids, sequences that are complementary

Answer 10

have matching nucleotides, A with T and C with G

Question 11

If a particular strain of bacteria is able to grow on media supplemented with a specific amino acid, but not able to grow on media without the amino acid, what can you conclude?

Answer 11

the bacteria is auxotrophic for the amino acid

Question 12

Which of the following terms is not an appropriate descriptor for the genome of the poliovirus?

Answer 12

negative sense RNA

Question 13

Exposing viral genetic material to host replicative enzymes is the least challenging for

Answer 13

bacteriophages

Question 14

Conjugation involves a special structure called a _____ which allows exchange of genetic material between a donor cell and recipient cell.

Answer 14

sex pilus

Question 15

The uptake of free DNA is referred to as _____.

Answer 15

transformation

Question 16

Structurally, the drug Fuzeon is similar to

Answer 16

unfolded HIV gp41

Question 17

Where do most RNA viruses replicate inside the host cell?

Answer 17

cytoplasm

Question 18

Mutations may result in:

Answer 18

• loss of gene function
• restoration of gene function lost previously
• modification of gene function
• ALL OF THESE CHOICES

Question 19

Bacteriophage lambda maintains the lysogenic phase of its life cycle when

Answer 19

high levels of cl and low levels of cro proteins are present in bacterial cells

Question 20

The difference between Hfr and F+ strains is

Answer 20

the fertility plasmid is integrated into the host cell chromosomal DNA in Hfr cells while the fertility plasmid is independent in F+ cells

Question 21

In the presence of tryptophan

Answer 21

genes of the trp operon are not expressed

Question 22

The activity of which enzyme would be impacted by mutations in the promoter region of a gene?

Answer 22

RNA polymerase

Question 23

The correct sequence of steps in the Sanger sequencing method is

Answer 23

clone DNA fragments into vectors- synthesize new DNA strands- separate newly synthesized DNA strands by gel electorphoresis

Question 24

What is a proteome?

Answer 24

the translational products encoded for by the genes within a genome

Question 25

Quorum sensing is an example of

Answer 25

• individual microbes working cooperatively
• the cumulative impact of the response of a large number of individual cells
• a chemical signaling system
• ALL OF THE CHOICES

Question 26

A research team wants to examine the gene expression of two genes in an organism, gene A and gene B. Upon completing a microarray, gene A fluoresces with greater intensity than gene B. What does this mean regarding these genes?

Answer 26

Gene A is expressed at a higher level than Gene B

Question 27

There is a mutation in the lacI repressor protein making it incapable of binding to the lac operon. What might result in this condition?

Answer 27

The positive regulation of the lac operon is disrupted

Question 28

Two dimensional polyacrylamide gel electrophoresis (2D-PAGE) separates proteins based on

Answer 28

isoelectric point and molecular mass

Question 29

What types of gene do you think might generate green dots in the DNA microarray pattern?

Answer 29

virulence genes for invasion of mammals

Question 30

What might be a familiar term to use in instead of “PCR adapters” as we examine the 454 pyrosequencing technique?​​

Answer 30

generic primer recognition sites