Exam 2 Wiley Plus Quizes Flashcards
Translation initiation depends on the ribosome binding to a specific region of the mRNA known as the
Shrine-Dalgarno sequence
DNA replication in bacteria is initiated at the
Ori site
When compared to a template strand of DNA, the corresponding RNA strand is
complementary
What is the correct sequence of protein action during DNA replication?
- DNA polymerase
- Single stranded binding proteins
- DNA ligase
- Helicase
- Primase
4-5-2-1-3
Hershey and Chase used 35S and 32P to distinguish between proteins and nucleic acids. Why not just use 15N?
Because nitrogen is found in both proteins and nucleic acids, so there would be no way to distinguish between them
Insertions and deletions are frameshift mutations T/F
true
When Hershey and Chase determined that a bacterial virus labeled with 35S did not enter the bacterium and give rise to a new generation of viral particles, they were able to eliminate _____ as the genetic material.
proteins
a missense mutation
results in an amino acid codon converting to a codon for a different amino acid
When the genetic code is described as redundant, this means
most amino acids are coded for by more than one codon.
In the language of nucleic acids, sequences that are complementary
have matching nucleotides, A with T and C with G
If a particular strain of bacteria is able to grow on media supplemented with a specific amino acid, but not able to grow on media without the amino acid, what can you conclude?
the bacteria is auxotrophic for the amino acid
Which of the following terms is not an appropriate descriptor for the genome of the poliovirus?
negative sense RNA
Exposing viral genetic material to host replicative enzymes is the least challenging for
bacteriophages
Conjugation involves a special structure called a _____ which allows exchange of genetic material between a donor cell and recipient cell.
sex pilus
The uptake of free DNA is referred to as _____.
transformation
Structurally, the drug Fuzeon is similar to
unfolded HIV gp41
Where do most RNA viruses replicate inside the host cell?
cytoplasm
Mutations may result in:
- loss of gene function
- restoration of gene function lost previously
- modification of gene function
- ALL OF THESE CHOICES
Bacteriophage lambda maintains the lysogenic phase of its life cycle when
high levels of cl and low levels of cro proteins are present in bacterial cells
The difference between Hfr and F+ strains is
the fertility plasmid is integrated into the host cell chromosomal DNA in Hfr cells while the fertility plasmid is independent in F+ cells
In the presence of tryptophan
genes of the trp operon are not expressed
The activity of which enzyme would be impacted by mutations in the promoter region of a gene?
RNA polymerase
The correct sequence of steps in the Sanger sequencing method is
clone DNA fragments into vectors- synthesize new DNA strands- separate newly synthesized DNA strands by gel electorphoresis
What is a proteome?
the translational products encoded for by the genes within a genome
Quorum sensing is an example of
- individual microbes working cooperatively
- the cumulative impact of the response of a large number of individual cells
- a chemical signaling system
- ALL OF THE CHOICES
A research team wants to examine the gene expression of two genes in an organism, gene A and gene B. Upon completing a microarray, gene A fluoresces with greater intensity than gene B. What does this mean regarding these genes?
Gene A is expressed at a higher level than Gene B
There is a mutation in the lacI repressor protein making it incapable of binding to the lac operon. What might result in this condition?
The positive regulation of the lac operon is disrupted
Two dimensional polyacrylamide gel electrophoresis (2D-PAGE) separates proteins based on
isoelectric point and molecular mass
What types of gene do you think might generate green dots in the DNA microarray pattern?
virulence genes for invasion of mammals
What might be a familiar term to use in instead of “PCR adapters” as we examine the 454 pyrosequencing technique?
generic primer recognition sites
