chapter 10 (from quizlet) Flashcards
Term for the process in which a peptide fragment expressed from a plasmid is able to combine with a peptide fragment expressed from the chromosome to form a functional peptide.
a-complementation
What is contained in an expression vector but not in a regular cloning vector?
strong promoter
A his-tag or strep-tag is part of the cloning area in expression vectors. How do these sequences function?
affinity sequences to aid in purification of the recombinant protein
What is a term for the Sanger method of DNA sequencing?
dideoxy sequencing
The Sanger method of sequencing uses what?
dideoxynucleotides, DNA polymerase, a short oligo primer, deoxynucleotides
the Sanger method of DNA sequencing has been automated and uses what?
a thermal-stable DNA polymerase to allow for multiple rounds of DNA synthesis
What is an open-reading frame?
the protein encoding sequence of a gene
Many scientists deposit their gene sequences in GenBank to make them publicly available to other scientist via the internet. Approximately how many base pairs have been deposited thus far?
greater than 10 million
What is transcriptome?
the transcripts encoded for by the genes within a genome
What was the Northern blot hybridization technique used to measure?
the transcriptional expression of a specific gene
Microarray analysis may be used to study what?
gene expression in cells grown under different conditions
Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) separates proteins based on what property?
size and charge
What can be used to determine protein structure?
X-ray crystallography and NMR
What is the isoelectric point of protein?
the pH at which a protein has no charge
What are paralogs?
Genes that arise from duplication event in an organism
