chapter 11 (from quizlet) Flashcards

1
Q

Are expression of genes that are constitutive regulated?

A

No, unregulated

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2
Q

What is an effector molecule?

A

A small molecule that binds to an enzyme to regulate its activity

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3
Q

How is chemotaxis regulated?

A

By a series of signal transduction events

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4
Q

What is the function of methyl-accepting chemotaxis proteins? (MCPs)

A

To sense the presence of a specific chemical

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5
Q

What is required for transcription that is regulated by positive control?

A

activator protein

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6
Q

The expression of genes required for biofilm formation occurs only after a cell has attached to a surface that is suitable for biofilm development. What is this type of gene expression called?

A

induction

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7
Q

What would characterize the phenotype of a CheY mutant?

A

smooth swimming

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8
Q

What would characterize the phenotype of a CheB mutant?

A

constant tumbling

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9
Q

During negative regulation of gene expression the repressor protein binds to the operator to inhibit transcription. What is the effector molecule that controls the activity of the repressor protein called?

A

corepressor or inducer

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10
Q

What controls the expression of the lac operon?

A

both repression and activation mechanisms

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11
Q

How will E. coli growing in a medium containing both lactose and glucose react?

A

by preferentially utilizing glucose first

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12
Q

Mutants that constitutively expressed β-galactosidase were placed into two different groups. Where were the mutations for constitutive expression located?

A

In either the lacI gene or operator region

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13
Q

When does repression of the lac operon occur?

A

When the repressor protein binds to the operator region.

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14
Q

A mutation in the lacI gene results in an active repressor protein that can no longer bind allolactose. Which would be true of the phenotype of the mutant strain?

A

it would repress β-galactosidase when lactose is present.

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15
Q

Isopropyl β-d-1- thiogalactopyranoside (IPTG), a lactose analog, acts as an inducer of the lac operon. What is its mode of action?

A

It inactivates the LacI repressor protein

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16
Q

Attenuation is a regulator mechanism in bacteria that relies on what?

A

transcription and translation occurring simultaneously

17
Q

What is typically involved in a two-component regulatory system?

A

histidine kinase and a response regulator

18
Q

What component of the two-component regulatory system frequently senses changes in the external environment?

A

histidine kinase

19
Q

The transfer of T-DNA into a plant cell by Agrobacterium tumefaciens is a highly regulated process. This process only occurs if a plant becomes wounded and excretes various compounds that are detected by A. tumefaciens for expression of its virulence genes. Regulation of virulence gene expression is under the control of which type of system?

A

A two-component system

20
Q

How is quorum sensing, a type of cell to cell communication, mediated?

A

by chemical signaling

21
Q

What autoinducer is used by many Gram-negative bacteria?

A

a homoserine lactone derivative

22
Q

Once the autoinducer excreted by Vibrio fischeri reaches a critical concentration, it is able to diffuse back into the cell. What is the next action?

A

bind to transciptional activator protein to activate transcription of the lux genes

23
Q

What is the term for a large group of genes whose expression is under the control of a single regulatory system?

A

regulon

24
Q

What is a “reporter gene”?

A

the fusion of a promoter region of interest to a gene whose product can easily be measured

25
Q

What is true of catabolite repression?

A

it results in diauxic growth

26
Q

The catabolite activator protein (CAP) activated transcription of the lac operon when it binds this coactivator

A

cylic-AMP

27
Q

What does sigma factor helps the RNA polymerase do?

A

identify the promoter region of a gene

28
Q

The use of a sigma factor of RNAP for promoter identification is a type of what?

A

global regulation

29
Q

Small non-coding RNA molecules (sRNA) affect gene expression at the level of:

A

translation