Exam 2 deck 6 Flashcards
Gene transfer
the movement of genetic info between organisms
Recombination
combining genes (DNA) from 2 different cells
Recombinant
cell resulting from recombination
vertical gene transfer
occurs during reproduction of between generations of cells
horizontal gene transfer
the transfer of genes between cells of the same generation
3 types of genetic transfer in bacteria
1) Transformation
2) Conjugation
3) Transduction
Griffith’s mouse experiment
Smooth— dead
rough—-lived
heat killed smooth—-lived
rough + heat killed smooth—-dead
Transformation
The uptake of naked DNA by a cell that is maintained in a heritable form
competent cells
cells that can take up naked DNA
-either degrade it or incorporate it into its own genome
Why are competent cells useful for genetically modifying bacteria
because you can give one the desired genes and it will spread it to those around it
Conjugation
Transfer of DNA via cell to cell contact