Exam 2 Flashcards
Transposable Elements
segments of DNA that have the capacity to move in the genome
Replicative transposition
retrotransposons. TE is duplicated, made into RNA, treverse transcribed to DNA
DNA is transposed/inserted somewhere in genome
copy and paste
Conservative transpotition
TE is cut out of chromosome inserted elsewhere
cut and paste
Genes found in transposable elements
transposase (endonuclease) that cuts DNA (excise)
Reverse transcriptase
repeats at ends of transposable elements
recombine into genome
terminal repeats
repeats at ends of transposable elements
TE recognize genome in…
distrupt reading frame -splice site nucletotides insertion in regulatory sequence (new expression pattern) fuse gene A and B together Messy
Transposable element types
autonomous
nonautonomous
Autonomous TE
can move by themselves
encode transposase and other genes required to move it
Nonautonomous TE
lack transposase gene -cannot move by themselves -need autonomous
Cell cycle
M phase
Interphase
M phase
mitotic phase
Interphase
chromosomes are being replicated in prep for cell division
There are checkpoints, proteins assemble, etc
carrying on normal cell function
Interphase subphases
G1 -gap phase
S phase
G2
G1 phase
each chromosome is composed of a single chromatid, containing 1 DNA sequence
S phase
synthesis phase.
each chromatid is replicated so that each chromosome contains two identical DNA molecules, called sister chromatids