Exam 2 Flashcards
all chemical reactions that occur within a cell
metabolism
proccess that use energy to synthesize and assemble “build-up”
anabolism
endergonic reaction
anabolism
process that capture and store energy by breakin down complex molecules into simlier ones
catabolism
Exergonic reaction
catabolism
releases energy and electrons
calabolism reaction
proteins that work in metabolism by lowering the energy of activation
enzymes
have specific three-dimensional shape and complex with substrate and act upon active site
enzyme
not used up in reaction rather recylced
enzyme
convert target molecule (substrate) into different molecule (product)
enzyme
speed up catabolic reactions in biological systems
enzyme
an enzymes co-factor is what
inorganic like a mineral
an enzymes co-enzyme is what
orgainic like vitamin
explain what is meant by enzymes are catalysts
they increase the rate of a chemical reaction without being affted by the reaction
cataylsts
speed up catabolic reactions in biological systems
energy needed to start a reaction
activation energy
apoenzyme
particular protein enzyme
inorganic substance like mineral
cofactor
organic substance like vitamin
coenzyme
specific three-demensional shape and complex with substrates they act upon
active site
target molecule for enzyme
substrate
end molecule for enzyme
product
gylcolysis
sugar splitting
can occur with or without oxygen
10 step pathway that convert 6 carbon glucose into 2 3 carbon pyruvate molecules
net yield of 2 ATP and 2 NADH
intermediate between glycolysis and krebs
pyruvate is decarboxylated (CO2 removed) and oxide toform acetate 2C
NADH produced
acetate joins with high energy CoA to form Acetyl CoA
Krebs cycle
Acetyl CoA joins cycle complete oxidation of glucose 6 NADH 2 FADH2 2 ATP
oxidative phosphorlation
- electrons in NADH and FADH2 are used in electron transport chain to drive synthesis of ATP
- electrons transport chain passes electrons down chain
- ATP synthase harvest energy released by electron transport chain
- protons pumped out during ET pass back through channels on the ATP synthase that phosphorylate ADP to ATP
final electron receptor of oxidative phosphorylation
oxygen
how many ATP per glucose molcule in oxidative phosphorylation
38
during anaerobic respiration some prokayotes can respire using inorganic molecules other than ??? as final electron receptor
oxygen
because anaerobic resp generates less energy than aerobic resp these organisms
grow slowly
less ATP per glucose molecule when compared to aerobic resp
anaerobic
does anaerobic resp use the electron transport chain
yes
final electron receptor is inorganic but not oxygen
anaerobic resp
describe how anaerobic resp is different from aerobic resp
anaerobic resp doesnt use oxygen and produces less ATP per glucose molecule
anaerobic resp is different from fermentation
fermentation is used in organisms that dont have an electron transport chain
fermentation final electron acceptor is an organic molecule
fermentation
- partial oxidation of sugar to release energy
- anaerobic process that releases energy from sugars by oxidation
- 2 ATP per glucose molecule
- used by organisms that cannot respire either because oxygen is not available or they do not have an electron transport chain
- produce lactic acid causes tooth decay and food spoilage
- ethanol - wine, beer, bread
- propionic acid - cheese flavor
- final electron receptor pyruvic acid
used by organisms that cannot respire either because oxygen is not available or they do not have an electron transport chain
fermentation
role of chemiosmosis in oxidative phosphorylation
using ion gradients to generate ATP
ATP synthases
make won food by reducing CO2
light = energy source; carbon dioxide as carbon source
photosynthetic bacteria- algae and green plants
produce oxygen
green and purple sulfer, and cynobacteria
photoautotrophs
using ready made organic molecules for food
light=energy source; organic compounds as carbon source
does not produce oxygen–anoxgenic
purple and green nonsulfer bacteria
photoheterotrophs
make own food by reducing CO2
inorganic compounds = energy source
carbon dioxide = carbon source
iron, sulfer, hydrogen and nitrifyling bacteria
chemoautotrophs
using ready made organic molecules for food
energy and carbon source = glucose
most all bacteria, all protozoans, fungi, and animals
chemoheterotrophs
incease in population mass (number of cells) rather than size of organism
growth
time for population of cells to double in number
doubling (generation) time
asexual reproduction in unicelluar organisms by division into 2 daughter cells
binary fission
cold loving - grow in cold water and soil
-5C -15C
found in artic and antartic
psychrophiles
cannot grow above 20C
obliagate psychrophiles
grow best at 20C but can grow above
facultative psychrophiles
grow at 0-35C
spoilage microoranism
staphylococcus aureas
listeria monocytogenes (hot dogs and bologna)
grow on refrigerated food and cause food- borne illness
pyschrotrophs
grow at 25C-45C
optimum at 37C
most disease causing bacteria in humans
E. coli
syphillis grows on hottest part of the body
leprosy grows slightly cooler than human trunk
Meophiles
heat loving - hot tub bacteria
grows at 45C - 70C
yogurt
Thermophiles
Extreme heat
grows best at 70C - 110C
archaea - hot springs, hyropthermal vents
Hyper thermphiles
minimal temp for bacterial to grow
minimal growth temp
what temp bacteria grow best at
optimal growth temp
max temp bacteria can grow
maximum growth temp
perferred pH of 5-8
neutrphiles
preferred pH <5.5
live in dry, acid soils around volcanoes
Acidophiles
preferred pH >8.5
live in alkaline lakes and soils
Alkalinophiles
importance of osmotic pressure to microbial growth
prokaryotes that can grow in high solute solutions increase their intrenal solute concentrations by either pumping ions into cell or synthesizing small organic compounds
component of amino acids, lipids, nucleic acids and sugars
carbon
component of all cellualar constitution
hydrogen
often rate limiting growth factor, component of all amino acids and nucleic acids
nitrogen
can grow in relatively high sugar solution
osmotolerant or osmophile
requires very high NaCl, some require >20%
extreme halophile
require high salt
obliage halophile
do not require salt but can tolerate salt up to about 2%
facultative halophile
dryness - fingi, dehydrated or dried food, beef jerky
xerophile
exist under extreme high pressure
pressure exerted by water (hydrostatic)
deep sea micbores
barophiles
absolute requirement for oxygen, genrate energy in aerobic respiration, catalase and superoxide dismutase
obligate aerobes
type of bacteria that grows at the very top of broth all packed together
obligate aerobes
cannot multiply if any oxygen is present, use anaerobic respiration/fermentation for energy, do not have catalase or superoxide dismutase
obligate anaerobes
grow dispersedly from bottom to middle
obligate anaerobes
indifferent in oxygen, can grow with oxygen presence but not use it to transform energy, do not use aerobic metabolism
aerotolerant anaerobes
grow dispersedly throughout broth
aerotolerant anaerobes
grow better with oxygen but can grow without it, use aerobic resp if oxygen is available and anaerobic or fermentation if it its not; have catalase and superoxide dismutase
faculative anaerobes
grow mainly toward the top of broth but some toward bottom
faculative anaerobes
require small amounts of oxygen (2%-10%) for aerobic resp, have small amounts of catalase
microaerophiles
grow in broth near the top all compacted together
microaerophiles
describe how oxygen can be fatal and how organisms protect themselves from toxic forms of oxygen
oxygen requirement/ tolerance reflects organisms energy-converting mechanisms (aerobic, anaerobic, and fermentation and its ability to detoxify oxygen derivatives
break down hydrogen peroxide, using a reducing agent
catalase
broken down by peroxidase, using a reducing agent
hydrogen peroxide
breaks down superoxide anion
superoxide dismutase
meaning of -phile
love of or friendship
requires certain environmental factors
obligate
are not as set in stone to enviromental factors and can wiggle one way or the other a little
facultative
antioxidant enzymes that most obliage anaerobes lack
catalase or superoxide dismutase
time for population of cells to double in number
generation time
number of cells not increasing; bacteria synthesizing macromolecules needed for growth; metabolism, new DNA
lag phase
cells divide at constant rate (exponential growth)
log phase
bacteris stop growing after they have used up all required nutrients or oxygen
cell growth = cell death
stationary phase
growth levels out, food is limited, metabolic waste is building up
stationary phase
number of viable cells decrease at a constant rate
death phase
transmission of genetic material from one generation to the next
heredity
necessary functions, organisms complete set of DNA, including all of its gene
genome
thread like strand of DNA that carries genes
chromosome
functional unit of DNA- piece of DNA that codes for either of polypeptide or protein
gene
different forms of genes found at the same place on a chromosome (brown and blue eyes)
allele
actual gene- complete heritable genetic idenity
Ex. TtBB
genotype
appearance of gene - discription of actual physical characteristics (height, hair, and eye color)
phenotype
nitrogenous bases pairs supported by a sugar phosphate backbone double-helix
DNA
origin, helicases, replication fork, DNA polymerase, leading strand, lagging strand, semiconservative replication
DNA
two main functions of DNA
replication- DNA, with all its genes, must be copied every time a cell divides
expression- genes on DNA must control characteristics
name the 4 nucleotides in DNA
thymine
adenine
guanine
cytosine
name the 4 nucleotides in RNA
uracil
adenine
guanine
cytosine
two strand of DNA are said to be antiparallel
two strands of DNA have opposite chemical polarity, sugar-phosphate backbones run in opposite directions
unzio DNA helix
separates the DNA into template strands
Helicase
enzyme used in replication of a new DNA strand from a single-stand template
DNA polymerase
allows DNA polymerase to bind and start replication
RNA primer
essential enzymes within all cells that seals breaks in the phosphate-sugar backbone of DNA- joining Okazaki fragments (lagging strand)
DNA ligase
replicated strand of DNA that grows continously without any gap
leading strand of DNA
its does not require DNA ligase
leading strand of DNA
direction of growth is 5’-3’
leading strand of DNA
only a single RNA primer is required
leading strand of DNA
template open in 3’-5’ direction
leading strand of DNA
replicated strand of DNA which is formed in short segments called Okazaki fragments
lagging strand of DNA
DNA ligase is required
lagging strand of DNA
direction of growth 3’-5’
lagging strand of DNA
okazaki fragment is 5’-3’
lagging strand of DNA
starting of each Okazaki fragments requires a new…
RNA primer
template open in 5’-3’
lagging strand
during DNA replication the two strand seperate and each strand has a complimentary strand bulit onto it this is referred to as…
semi-conservative
semi-conservative DNA replication
each new DNA molecule is composed of one conserved strand from the original molecule and one new strand
what is transcription
DNA is transcribed as RNA to produce mRNA, which carries information needed for protein synthesis
- building RNA copy of DNA
promoter
start of single RNA polymer
function of RNA polymerase
find promoter on gene, DNA undwind, transcribe DNA to RNA
in prokaryotic cells where does transcription occur
cytoplsam
in eukaryotic cells where does transcription occue
nucleus
what is translation
mRNA formed in transcription is transported out of the nucleus to ribosome; mRNA directs protein synthseis with assisatnce of tRNA
transcription
decoding of mRNa message into protein chain
coverts mRNA sequence into amino acids that form proteins
transcription
mRNA
messanger RNA
carries “gene” info for amino acid sequence
tRNA
transfer RNA carry amino acod to ribosomes during translastion
rRNA
ribosomal RNA found only in ribosomes
what are codons
3 mRNA base that represnts 1 amino acid
what is genetic code
set of ruless by which info encoded within genetic material is translasted into proteins by living cells
start codon
start translation
stop codon
stop translation
any change in DNA that is heritable
mutation
point mutation
change in base of DNA
change in one base either induced or spontaneous
point mutation
induced point mutation
something in the environmet; radiation X-rays, and chemicals
2 types of frameshift mutation
insertion and deletion
inserts one or bases of DNA
insertion frameshift mutation
removes one or more bases of DNA
deletion frameshift mutation
silent mutation
no visable change
missense mutation
change in amino acid (good, bad, ugly, or neutral)
nonsense mutation
put stop codon where it doesnt belong
what is mutagen
physical or chemical agent that changes the genestic material