Exam 2 Flashcards
roles of the large intestine
- microbial fermentation
- absorption
- expels waste
define hindgut microbial fermentation
microbes fermenting “leftovers”, what the animal could not absorb itself (mostly fibers)
What does the large intestine absorb?
main: VFAs
and water (electrolytes - macro minerals)
What are crypts in the large intestine?
stem cells
define feces
expelled in waste, what can’t be digested/absorbed
define endogenous losses
expelled in waste, sloughed cells (dead epithelium, bile, enzymes, etc.)
immune functions of the large intestine
motility, flushing it out
What are the structural parts of the large intestine?
- mucosal tissue
- cecum
- large colon
- small colon
- rectum
structure of the cecum
a “blind sac”, goes to no where, lots of fermentation
What is the structure of the large colon?
leads to the rectum
What is the structure of the small colon?
spiral
What is the structure and function of the rectum?
- highly controlled sphincter
- nervous regulation
What is the relationship between feces and water absorption?
the more water absorbed in the intestine, the harder and smaller the poop
Why does the large intestine have lower surface area than the small intestine?
there are no vili in the large intestine, so there is a lower surface area
Where is the pancreas located?
between the stomach and small intestine
What is the function of the pancreas?
synthesize and secrete digestive enzymes (80-90% - exocrine) and metabolic hormones (5-20% - endocrine) and secretes bicarbonate into the small intestine
What is the function of the digestive enzymes?
the pancreas secretes them in the duodenum into the gi tract (acts before small intestine enzymes)
- examples: proteases, lipases, amylases
What is the function of metabolic hormones?
some regulate the gi tract, others metabolism
- example: insulin
What is the function of bicarbonate in the small intestine?
- buffer (HCl)
- elevate the ph closer to 7 (neutral)
What is the function of the liver?
- bile
- metabolism
- storage of some vitamins and minerals
- short term energy storage (glycogen)
- detoxification
Where is bile stored?
gallbladder, unless the animal does not have one then it is stored in the liver
What is the function of bile?
fat emulsification
What is the function of detoxification in the liver?
metabolizing “non-nutrients”
–> hormones, alcohol, drugs
What is the function of a gallbladder?
- found on the liver
- stores and releases in bile
(not present in all species)
What is the function of the kidneys?
not attached to gi tract, no direct secretion, but handles things the gi tract brings in
- detoxification (urea)
- excretion of waste (urine)
- water balance (electrolytes)
What is the function of the brain in the gi tract?
regulates the gi tract because it controls the nervous system
- highly involved in satiety
- produces metabolic and gi hormones
- target of hormones
define satiety
fullness, stop eating
define fermentation
microbial metabolism of organic compounds to obtain energy in and anaerobic environment
What are microbes?
bacteria (most) and Protozoa (largest in size)
What organic compounds are being fermented?
- carbohydrates
- carbon skeletons of AA
- glycerol backbones of triglycerides
What are the products of fermentation?
- ATP (for the microbes)
- coproducts (good=VFA, bad=CO2, CH4)
Where is fermentation located in a ruminant?
rumen, reticulum, omasum (and large intestine)
What are the 4 steps in the ruminant gi tract?
- RRO (fermentation) –> complex foregut
- gastric stomach
- small intestine
- large intestine
What is the importance of fermentation happening before the animal does digestion and absorption?
- gives microbes a first chance to ferment nutrients
- microbes from RRO are food (protein) for the animal
How are microbes killed?
HCl, they pass out/die and go into the stomach
Why is feeding a ruminant more complicated than feeding a monogastric?
feeding the microbes first and hoping we can get the nutrients to the animal
define coprophagy.
eating feces
define regurgitation.
throwing up, before rumination
deine rumination.
rechewing previously consumed feed (mostly fibers and long pieces of forage)
Why do ruminants ruminate?
to decrease particle size, increase surface area, and increase digestion
define eructation.
rid of gases, belch (prevent bloat)
define bloat and how it can become an issue
accumulation of gas, if an animal gets bloated they can suffocate and die
Where is most of the digesta located?
ruminant
in the 4 stomach sections?
Does a ruminants stomach have a slow or fast passage rate?
slow passage rate
What is the order of the sections of the stomach complex?
- reticulo-rumen
- omasum
- abomasum (gastric stomach
What are the layers of the stomach complex?
TOP - gas cap (CH4, CO2)
- fiber mat
- liquid (microbes and small feed particles)
BOTTOM - heavy material (rocks, metal, etc)
What is the fiber mat in the stomach complex?
fiber floats on top of the stomach liquid, makes it easier to regurg into the esophagus
What is the function of the pillar structures in the stomach complex?
promotes good mixing of the rumen and sections the stomach complex
Why are the reticulum and rumen called the reticulo-rumen?
the rumen and reticulum are continuous
What are the functions of the reticulo-rumen?
- main site of microbial fermentation
- house microbes
- absorption of VFAs and ammonia
Where is the main site of microbial fermentation?
ruminant
reticulo-rumen
What makes the environment of the reticulo-rumen ideal for microbial fermentation?
- lots of water
- warm (102-108 degrees F)
- ph is variable
- no oxygen
Why is there a honeycomb structure in the reticulo-rumen?
we don’t know why
How do we study microbes?
microbes are difficult to study because it is hard to simulate or enter their exact environment. However, now we can genotype them.
What are papillae?
structures in the reticulo-rumen that increase surface area and give microbes a home
Why would papillae change shape/size?
- top of the rumen has loose bumps (less absorption)
- mid-bottom of rumen have massive papillae because they are absorbing lots of nutrients
- diet: more fermentation=large papillae, not eating=small papillae
Is the rumen motile?
the rumen is very thick and muscular and constantly moving
describe the rumen epithelium
- covered in papillae
- NOT mucosal
What is the structure of the omasum?
it is a hard structure with NO papillae, but LOTS of nubs/folded tissue
What is the function of the omasum?
- controls passage to abomasum
- absorption of VFAs and NH3 (not yet absorbed)
Why does the omasum have so many folds?
to increase surface area, to increase absorption
What is the structure of the tissue in the abomasum?
it is mucosal
Which part of the stomach complex gets a continuous digesta flow?
abomasum
In a ruminant, what parts of the gi tract are similar to a monogastric?
- small intestine
- large intestine
Why would the stomach complex shrink?
if there is less nutrients coming in
- (passage rate will also decrease)
What are the microbe types named after?
what they do or break down
What are the goals of a microbe?
- survive
- grow
- reproduce
all of these use energy!! growth and reproduction uses AA and FA.
What are microbes made up of?
protein and fats
Where do microbes get their ATP from?
carbon skeletons, carbohydrates, and glycerol backbones
What carbohydrates can microbes degrade with enzymes?
- fiber
- starch
- sugar
Which type of carbohydrate do fibrolytic microbes degrade that have beta linkages?
fiber
Which type of carbohydrate do amyolitic microbes degrade that have alpha linkages?
starch
What is the main product of microbial fermentation of monosaccharides?
ATP
What are the coproducts of microbial fermentation of monosaccharides?
- VFAs
- CO2
- CH4
What is the structure of a VFA?
short chained fatty acid (2-8 chains)
What is a VFA?
- Volatile Fatty Acid
- not needed by microbes but can be used by the animal in glucogenoisis (for energy)
Are VFAs soluble in water?
YES, although they are fatty acids they have short chains
Is the ph of VFAs low or high?
low, they are acidic
Where are VFAs absorbed?
in the RRO epithelium (and large intestine)
If a ruminant is eating more starch, would it have a high or low ph?
LOW PH (5.5-6.5) because more VFAs are being produced and there is a quick rate of absorption
What are common VFAs?
- butyrate
- acetate
- propionate
If a ruminant is eating more fiber what is their ph?
NEUTRAL because fibers are more neutral materials
When changing an animals diet, why does it need to be done slowly?
the microbes need time to adapt (when there are less substrates for a type of microbes they will regress, so you need to introduce the new diet slowly to let the microbe population build back up)
What is the speed of fiber fermentation?
slower, fibers are harder to break down (chew, metabolize, lignin)
Where are fibers broken down if they escape the RRO?
fermented in the large intestine
What microbes and enzyme(s) break down fibers?
- fibrolytic
- cellulose
What microbes and enzymes break down sugars and starches?
- amyolytic
- amylase, sucrase, lactase
(can be used WITHOUT microbes)
What is the speed of starch/sugar fermentation?
faster, VFAs are produced rapidly
What is sub acute acidosis?
an animal has very low ph, caused by feeding too many starches/sugars
Where are starches/sugars digested if they bypass RRO?
small intestine and absorbed
(does not happen often)
What lipids do microbes hydrolyze?
triglycerides and phospholipids (to yield glycerol and FA)
What is yielded when microbes break down lipids?
fatty acids and glycerol (12-20 chained)
What is the product of fermenting a glycerol backbone from a triglyceride?
VFA
Can microbes break down fatty acids?
NO
3 things microbes can do to a fatty acid:
- biohydrogenate
- isomerize
- incorporated into the microbe (growth and reproduction)