EXAM 15: serology Flashcards
1
Q
you can identify an unknown reagent using
A
a known antigen or antibody
2
Q
serology
A
- the study into the nature and behavior of serum antibodies
- an important feature is the lock and key relationship
3
Q
titration
A
- a procedure used to determine how much antibody is present in a serum sample
- is the greatest dilution of antibody that will still cause a visible reaction
- agglutination or precipitation indicates an antibody/antigen reaction
- the highest dilution that has a reaction is called a titer
4
Q
agglutination
A
- clumping of the antigen in the presence of the specific antibody
- antigens that do this are small particles that stick together when they come in contact with a specific antibody
5
Q
what are the types of agglutination reactions
A
- tube agglutination
- rapid slide agglutination
- brucella milk ring test
6
Q
precipitation reactions
A
- antigen molecules are much smaller
- the antigen is part of the solution and becomes visible only after it has reacted with an antibody
- product is a fine precipitate
- commonly used to detect viral antibodies (therefore can be used to help diagnose disease)
7
Q
Gel diffusion
A
- a precipitation reaction
- The test is done in a petri dish
- wells are cut into the agar, and the antigens and antibodies are put into it. They diffuse into the agar and where they meet there will be a fine line of precipitate
- can be used to detect viral disease
8
Q
serotyping
A
differentiating based on cell surface differences
9
Q
fluorescent antibody
A
are reactions that make use of a labeled antibody
- used to detect the rabies virus
10
Q
Coggins test
A
- gell diffusion test
- designed to detect antibodies for equine infectious anemia (EIA)
- will detect the antibodies within 45 days
- very serious and all horses must be tested
11
Q
What is commonly called ‘swamp fever’
A
equine infectious anemia
12
Q
hemagglutination
A
the clumping or sticking together of RBC
13
Q
direct hemagglutination
A
- caused by antibody
- can be an isoantibody (natural) or a hemagglutinin (antibody produced in experimental animals)
14
Q
viral hemagglutination
A
some viruses which have the ability to combine on the surface of RBC cause these cells to stick together
15
Q
viral hemagglutination inhibition test
A
- utilizes specific antibodies to neutralize the virus and inhibit the reaction
- has diagnostic value, it can reliably tell the vet if a certain antibody is present in an animal
