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MY Bacteriology exam 2 > Escherichia > Flashcards

Escherichia Flashcards

1
Q

What is the single most common facultative anaerobic gram negative bacteria that is part of the normal flora in the GI tract of animals

A

Escherichia Coli

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2
Q

E. Coli is motile with peritrichous _____ and _____

A

flagella and fimbriate

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3
Q

What pathogenesis does E. coli cause in almost every species it infects?

A

enteritis and septicemia

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4
Q

What pathogenesis does E. coli cause in pigs?

A

edema disease

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5
Q

True/False: E. coli causes an opportunistic infections of canine pyometra and cystitis as well as ruminant mastitis

A

True

*Staph aureus, Strep agalatia, and E coli are the causitive agents of mastitis

side note (Klebsiella, nocardia, truperella, and listeria are also possible causative agents of mastitis)

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6
Q

It is difficult to make vaccines against E. coli because the ___ antigens, that are part of the gram negative cell wall, create a lot of variability

A

O antigens

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7
Q

______ is released from the E. coli bacteria when they die and can lead to fever and septic shock in the animals

A

Lipid A

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8
Q

E. coli can have capsules (____ antigen), flagella (____antigen) and adhesins (___antigen)

A

capsule- K antigen

Flagella- H antigen

Adhesins- F or P antigen

*lots of different serotypes due to these possible antigens

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9
Q

finbria and pili are the adhesins that promote adherence to ____ on the epithelial cells of the intestinal tract and other target cells

A

glycoproteins

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10
Q

What is the most important immune component against E. coli?

A

The normal flora of the GI tract

*extremely important to avoid binding of these types of bacteria

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11
Q

_____(ETEC) is an E. coli adhesion that causes enteric disease and releases a toxin. This is for adhesion in the _____

A

enterotoxogenic, small intestine

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12
Q

_____(EIEC) mediates attach to the _____ cells to penetrate and cause dissemination throughout the body.

A

enteroinvasive, small intestine

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13
Q

_____(EAEC) forms a biofilm around the lumen of the _____ and does not allow for normal absorption and secretion, leading to diarrhea

A

enteroaggregative, small intestine

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14
Q

_____(EPEC) binds to the _____ in order to highjack the enterocyte and completely destroy the microvilli causing attaching and effacing lesions

A

enteropathogenic, small intestine

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15
Q

Enteropathogenic adhesions in the small intestine has a ____ forming pilus

A

bundle

Bfp

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16
Q

_____(EHEC) is an adhesion that utilized outer membrane protein A to bind to the _____. Causing attaching and effacing lesions

A

Enterohemorrhagic, large intestine

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17
Q

The enterohemorrhagic adhesions has ____membrane proteins

A

outer

OmpA

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18
Q

Enterohemorrhagic E. coli secretes what toxin?

A

Shiga-like toxin

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19
Q

Enterotoxins results in deregulation of water and electrolyte secretion resulting in ____ in neonates

A

diarrhea

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20
Q

How many toxins are produced by the enterotoxic E.coli?

A

three

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21
Q

_____ toxin binds to the endothelial cells and causes protein synthesis inhibition and death of the endothelial cells resulting in bloody diarrhea

A

Shiga-like toxin

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22
Q

Shiga like toxin inhibits ____

A

protein synthesis

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23
Q

Enteroinvasive E. coli produces a _____ necrotizing factor (CNF) that mediates the uptake of the bacteria by epithelial cells so they can disseminate

A

cytotoxic

24
Q

______ encoded toxin is present in many different kinds of E. coli and cause damage to endothelial cells and results in diarrhea

A

Plasmid

25
Q

Enterohemolysis is present in ______ E. coli

A

enterohemorrhagic

26
Q

_______ is a secretion system with needle like structures that come out of the cell wall of the bacteria and secrete effector proteins into the extracellular environment or into host cells.

A

Type III secretion system

27
Q

True/False: E. coli are part of the normal flora of the GI tract

A

True

28
Q

How is E. coli transmitted?

A

fecal oral route

29
Q

Most strains of E. coli do not cause disease, however they can _____virulence factors via transduction, conjugation, and transformation

A

Acquire

30
Q

Enterotoxigenic diarrhea is present more in ____

A

neonates

31
Q

If you visit a swine farm where there is a report of an outbreak on neonate pigs of watery diarrhea. You isolate a gram negative organism. What is at the top of your differentials?

A

Enterotoxogenic E. coli

32
Q

In Enterotoxigenic Diarrhea, the E. Coli needs to produce both ____ and _____

A

adhesins and enterotoxins

*needs to adhere to enterocytes of the small intestine

33
Q

Enterotoxigenic E. coli leads to ____diarrhea

A

watery

34
Q

True/False: Large bowel cells are susceptible to the enterotoxigenic toxin

A

False

LARGE BOWEL CELLS ARE NOT SUSCEPTIBLE

35
Q

True/False: Enterotoxigenic diarrhea leads to high mortality unless fluid and elecrolyte imbalance is corrected

A

True

36
Q

Enteropathogenic E coli causes ____ and _____lesions

A

attaching and effacing lesions in the GI tract

*hallmark of the disease

37
Q

_____ is responsible for intimate attachments via Tir with enteropathogenic E. coli

A

intimin

38
Q

Once Enteropathogenic E. coli binds to the enterocytes that utilize _____systems that produce effacing lesions and diarrhea

A

Type III secretion system

39
Q

_____ brings the bacteria very close to the enterocyte, which causes the collapse of the microvilli

A

intimin

40
Q

Enterohemorragic E. coli causes attaching and effacing lesions in the _____ intestine

A

large

41
Q

What is the main virulence factor of Enterohemorragic E. coli?

A

Shiga- like toxin

42
Q

Enterohemorragic E. coli causes ____ diarrhea

A

hemorrhagic (bloody)

43
Q

Enterohemorragic E. coli causes _____ in humans as a result of the shiga-like toxin

A

hemolytic uremic syndrome

44
Q

Enterohemorragic E. coli has ____ potential. Need to make sure to cook your meat!

A

zoonotic

45
Q

Enteroinvasive E. coli can be caused in neonates due to inadequate _____

A

colostrum

46
Q

_______ E. coli posses CNF-1 that is very important virulence factor. CNF induces the bacterial uptake into the host cell so that it can disseminate

A

Enteroinvasive E. coli

47
Q

The _____ is a very important virulence factor for Enteroinvasive E. coli so that it can escape phagocytosis and complement mediated lysis

A

capsule

48
Q

_____ disease is often a fatal enterotoxemia of weaned pigs

A

Edema

49
Q

What is the causative agent of edema disease in pigs?

A

enterohemorrhagic E. coli

*causes important mortality events in swine and effects the swine industry

50
Q

What is the most important virulence factor in edema disease?

A

Shiga-like toxin

*extensive generalized edema

51
Q

_____ of fowl is a widespread disease in birds that can cause embryonic death and is economically important for the poultry industry

A

colibacillosis

52
Q

True/False: it is imperative that the dam be exposed to microorganisms before partuition for antibodies to be made for secretion into colostrum

A

True

53
Q

You are able to grow E. coli in Blood agar and Maconkey agar. Ecoli is lactose ____ in Maconkey agar

A

positive

*will look purple

54
Q

What does E. coli look like under the microscope

A

Gram negative rods with rounded ends

55
Q

What agar is made specificaly for identification of E. coli?

A

Eosin Methylene blue agar

*will have green metallic sheen when positive

56
Q

True/False: Fluid and elecrolyte therapy is a good treatment for E.coli

A

True

MY Bacteriology exam 2 (18 decks)

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