Enzymology, lipid, cardiac biomarkers Flashcards
What are “leakage enzymes” ?
May be in cytosol, organelles, or both
Enzymes escape from the cell as a result of injury to cell membranes or organelles
What are “induced enzymes”?
Usually attached to membranes, rarely due to cell injury
Increased are usually due to increased production
What are the liver leakage enzymes?
ALT
AST
SDH (large animal)
GLDH (large animal)
What are induced enzymes associated with the liver?
ALP and GGT
T/F: serum ALT will be increased more with sublethal damage then it is with necrosis
True
What enzyme is muscle specific, an increase indicates damage to muscular tissue
Creatine kinase (CK)
Asparatate aminotransferase has the highest activity in what organs?
Skeletal and cardiac muscle
Hepatocytes
What is the half life of creatinine kinase (CK)?
Rapidly peaks in 6-12hrs following injury and has a half life of 2-4hrs
Is AST a good indicator of muscle injury?
Can be
Creatinine kinase is muscle specific but has a very short half life. AST stays elevated longer in muscle injury, but is not muscle specific
_________ is a liver specific enzyme in dogs and cats but can be incresed with severe muscle damage
ALT
__________ is released from dead/dying muscle. It is not protein bound in the blood and is excreted in the urine
Myoglobin
Dipstick positive for blood/hemoglobin. How can you distinguish myoglobin from hemoglobin in the urine?
Ammonium sulfate precipitation test
-hemoglobin precipitates, myoglobin does not
Anemia -> hemoglobin
CK increased -> myoglobin
Triglycerides synthesized in the liver are not water soluble and must be attached to proteins in the blood
Very low density lipoprotein (VLDL)
What are the uses of cholesterol?
Produce steroid hormones
Component of cell membranes
Precursor to bile acids
What are the most commonly measured blood lipids?
Triglyceride and cholesterol