Enzymology, lipid, cardiac biomarkers Flashcards

1
Q

What are “leakage enzymes” ?

A

May be in cytosol, organelles, or both

Enzymes escape from the cell as a result of injury to cell membranes or organelles

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2
Q

What are “induced enzymes”?

A

Usually attached to membranes, rarely due to cell injury

Increased are usually due to increased production

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3
Q

What are the liver leakage enzymes?

A

ALT
AST
SDH (large animal)
GLDH (large animal)

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4
Q

What are induced enzymes associated with the liver?

A

ALP and GGT

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5
Q

T/F: serum ALT will be increased more with sublethal damage then it is with necrosis

A

True

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6
Q

What enzyme is muscle specific, an increase indicates damage to muscular tissue

A

Creatine kinase (CK)

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7
Q

Asparatate aminotransferase has the highest activity in what organs?

A

Skeletal and cardiac muscle

Hepatocytes

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8
Q

What is the half life of creatinine kinase (CK)?

A

Rapidly peaks in 6-12hrs following injury and has a half life of 2-4hrs

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9
Q

Is AST a good indicator of muscle injury?

A

Can be

Creatinine kinase is muscle specific but has a very short half life. AST stays elevated longer in muscle injury, but is not muscle specific

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10
Q

_________ is a liver specific enzyme in dogs and cats but can be incresed with severe muscle damage

A

ALT

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11
Q

__________ is released from dead/dying muscle. It is not protein bound in the blood and is excreted in the urine

A

Myoglobin

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12
Q

Dipstick positive for blood/hemoglobin. How can you distinguish myoglobin from hemoglobin in the urine?

A

Ammonium sulfate precipitation test
-hemoglobin precipitates, myoglobin does not

Anemia -> hemoglobin

CK increased -> myoglobin

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13
Q

Triglycerides synthesized in the liver are not water soluble and must be attached to proteins in the blood

A

Very low density lipoprotein (VLDL)

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14
Q

What are the uses of cholesterol?

A

Produce steroid hormones

Component of cell membranes

Precursor to bile acids

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15
Q

What are the most commonly measured blood lipids?

A

Triglyceride and cholesterol

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16
Q

A primary hyperlipidemia is due to ?

A

Inherited disorder

17
Q

Secondary hyperlipidemia is associated with ??

A

Endocrine or metabolic disorders

18
Q

T/F: hyperlipidemia always results in lipemia

A

False

Hyperlipidemia -> trigyclerides and cholesterol

Lipemia–> chylomicroins or VLDL

19
Q

What do you see commonly in small equids during starvation or chronic illness secondary to negative energy balance?

A

Equine hyperlipidemia

20
Q

What are primary hyperlipidemia ? What breed of dogs is this seen most frequently?

A

Inherited defect in lipoprotein lipase or apoprotein CII

Miniature schnauzers

21
Q

What diseases are associated with secondary hyperlipidemia?

A
Hypothyroidism 
Diabetes mellitus 
Hyperadrenocorticism 
Pancreatitis 
Hepatic disease 
Nephrotic syndrome
22
Q

What is nephrotic syndrome?

A

Hypercholesterolemia is a common finding in dogs and cats with protein losing glomerulonephropathy

  • hypoalbuminemia
  • proteinuria
  • hypercholesterolemia
  • edema or ascities
23
Q

What can cause a hypolipidemia?

A

Liver failure - hypocholesterolemia

Maldigestion/malabsorption

Protein losing enteropathy

Starvation

24
Q

During starvation, ________ can occur due to fat mobilization from adipose tissue exceeding the ability of the liver to oxidase the fat

A

Ketosis

25
Q

Lactating dairy cows, anorexic obese cats, and anorexic small equids can have ______________ because of increased hepatic uptake of LCFA plus impaired hepatic synthesis of triglyceride rich lipoproteins

A

Hepatic lipidosis

26
Q

What are the two main tests for cardiac function/damage

A

Myocardial cell injury test - leakage markers

Specific cardiac function proteins - functional markers

27
Q

Cardiac biomarkers should have what prerequisites?

A

Quick turn around time
High sensitivity and specificity for cardiac injury
Affordable
Screening test for asymptomatic heart disease
Distinguish cardiac vs non cardiac dyspena
Identify myocardial injury

28
Q

Troponin complex regulates??

A

Calcium-mediated interaction of myosin and actin

29
Q

The tropnonin complex in made up of what 3 proteins ?

A

Cardiac troponin I
Cardiac troponin T
Cardiac troponin C

30
Q

What one of the troponin proteins is inhibitory and prevents contraction when calcium is not present and is the most diagnostically useful?

A

Troponin I

31
Q

Increasing troponin concentrations suggest?

A

Worsening condition of the heart

Eg congestive heart failure, pericardial disease, or cardiac injury due to sepsis, doxorubicin toxicity, gastric-dilatation-volvulus, and myocarditis

32
Q

What is the most useful functional marker for the heart

A

Natriuretic peptide (hormone regulating salt and water homeostatis and BP control )

33
Q

Brain natriuretic peptide is produced by the ________

A

Ventricles

34
Q

What is increased in production with increased ventricular wall tension hypertrophy, or myocardial dysfunction

A

Brain natriuretic peptide (BNP)

Cleaved to pro-BNP where the N terminal is cleaved

N-Terminal has a long halflife and what is measured

35
Q

Under what conditions can plasma NT-proBNP be increased?

A
Ventricular hypertrophy 
Tachycardia 
Hypoxia 
Expanded fluid volume 
Reduced renal clearance of the peptide