Enzyme-Linked Immunosorbent Assay (ELISA)

Question 1

immunological assay commonly used to measure antibodies, antigens, proteins and glycoproteins in biological samples

Answer 1

enzyme-linked immunosorbent assay (ELISA)

Question 2

what is included in enzyme-linked immunosorbent assay (ELISA)

Answer 2

enzyme-labeled antigen or antibody

Question 3

how is enzyme activity measured

Answer 3

colorimetrically

Question 4

depending on the antigen-antibody combination, the assay is called a what?

Answer 4

• direct ELISA
• indirect ELISA
• sandwich ELISA
• competitive ELISA

Question 5

General components of enzyme-linked immunosorbent assay (ELISA)

Answer 5

1. microtiter plate
2. coating antigen or antibody
3. blocking agent
4. sample or analyte
5. detection antibody
6. substrate solution
7. wash buffer
8. stop solution (optional)

Question 6

application of enzyme-linked immunosorbent assay (ELISA) example

Answer 6

diagnosis of Helicobacter pylori