Enzyme Laboratory Methods Flashcards

1
Q

What is the Laboratory method of AST

A

Karmen Method

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2
Q

Karmen Method is the laboratory method of what enzyme

A

AST

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3
Q

Karmen Method Indicator

A

Malate Dehydrogenase

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4
Q

What is the Laboratory method of ALT

A

Reitman Frankel Method
Wacker Method

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5
Q

This method form ketohydrazone (Golden Brown Color)

A

Reitman Frankel Method

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6
Q

This method reacts with 2,4 dinitrophenylhydrazine

A

Reitman Frankel Method

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7
Q

Reitman Frankel Method what enzyme

A

ALT

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8
Q

Wacker Method what enzyme

A

ALT

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9
Q

Indicator of Wacker Method

A

Lactate Dehydrogenase

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10
Q

LDH laboratory methods

A

Wacker Method (Forward)
Wrobleuwski La Due Method (Reverse)

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11
Q

Wacker Method (Forward reaction) what enzyme

A

LDH

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12
Q

end product of Wacker Method (Forward; Lactate to pyruvate) in LDH

A

BLUE PURPLE

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13
Q

Reaction pH of Wacker method in LDH

A

8.8

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14
Q

Wrobleuwski La Due Method (Reverse) what enzyme

A

LDH

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15
Q

Reaction pH of Wrobleuwski La Due Method (Reverse; Pyruvate to Lactate) in LDH

A

7.2

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16
Q

ALP laboratory methods

A

Shinowara Jones Reinhart
King Armstrong
Bessey Lowry Brock
Bowers Mcomb

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17
Q

Substrate of Shinowara Jones Reinhart(ALP)

A

beta-glycerophosphate

18
Q

Substrate of King Armstrong (ALP)

A

a-phenylphospate

19
Q

Substrate of Bessey Lowry Brock(ALP)

A

p-bnitrophenyphosphate

20
Q

CK laboratory methods

A

Tanzer Gilvarg Method
Oliver Rosalki Method

20
Q

Substrate of Bowers Mcomb (ALP)

A

p-bnitrophenyphosphate

21
Q

GGT laboratory methods

A

Szasz Method

21
Q

Oliver-Rosalki Method (reverse) CK Indicator

A

Hexokinase and G6PD

22
Q

Tanzer Gilvarg Method Creatine-creatinephosphate (FORWARD) CK Indicator

A

Pyruvate kinase and LDH

22
Szasz Method substrate (GGT)
gamma-p-nitroanilide
23
ACP laboratory methods
Bodansky Roy Hudson Gutman King Armstrong
24
Bodansky (ACP) substrate
beta-glycerophosphate
25
Lab method Specific for PROSTATIC ACP
Roy
26
Roy (ACP) substrate
Thymolphthalein monophosphate
27
Hudson (ACP) substrate
p-nitrophenyl phosphate
28
Gutman King Armstrong (ACP) substrate
phenyl phosphate
29
Lipase Labortaroy methods
Cherry Crandall (reference method) Tietz
30
Cherry Crandall (Lipase) Substrate: Indicator: Endpoint Color:
50% olive oil phenolphthalein Pink
31
Tietz (Lipase) Substrate: Indicator: Endpoint Color:
50% olive oil Thymolphthalein Blue
32
Amylase laboratory methods
Amyloclastic Saccharogenic Chromogenic
33
Measures disappearance of starch substrate (Amylase) Lab method
Amyloclastic
34
Measure appearance of product (Amylase) Lab method
Saccharogenic
35
Measure chromogenic dye coupled with product (Amylase) Lab method
Chromogenic
36
Aldolase laboratory method
Sibley Lehninger Method
37
Glucose-6 Phosphate Dehydrogenase Laboratory method
Mark et al
38
Sibley Lehninger Method (Aldolase) substrate
Fructose 1,6 diphosphate