ENHANCEMENT CLASS: SPECTROPHOTOMETRY

Question 1

Measurement of the intensity of light at selected wavelengths.

Answer 1

SPECTROPHOTOMETRY

Question 2

Is the distance between two peaks as the light travels in a wavelike manner

Answer 2

WAVELENGHT

Question 3

According to ________, the concentration of a substance is directly proportional to the amount of light absorbed or inversely proportional to the logarithm of
the transmitted light.

Answer 3

Beer-lambert law

Question 4

Equation of Beer-lambert law:

Answer 4

A = ebc

Question 5

Is used to measure the light transmitted by a solution to determine the concentration of the light-absorbing substance in the solution.

Answer 5

SPECTROPHOTOMETER

Question 6

Light source

Answer 6

bulb

Question 7

Visible and near infrared region

Answer 7

Incandescent Tungsten/Tungsten iodide lamp

Question 8

Ultraviolet (UV)

Answer 8

Deuterium lamp
Mercury-arc lamp
Hydrogen lamp
Xenon arc lamps

Question 9

• Isolates radiant energy (light) of a desired wavelength.

Answer 9

MONOCHROMATOR

Question 10

MONOCHROMATOR Types

Answer 10

o Filters
o Prism
o Diffraction gratings

Question 11

• A small vessel used to hold a liquid sample to be analyzed in the light path of a spectrometer
• It may be round, square or rectangular.

Answer 11

SAMPE CELL (CUVET/CUVETTE)

Question 12

SAMPE CELL (CUVET/CUVETTE) TYPES

Answer 12

o Glass
o Silica (Quartz)
o Plastic

Question 13

A device that converts the transmitted radiant energy
into an equivalent amount of electrical energy.

Answer 13

PHOTODETECTORS

Question 14

PHOTODETECTORS TYPES

Answer 14

➢ Photocell
➢ Phototube
➢ Photomultiplier
- Most commonly used