ENHANCEMENT CLASS: FUNDAMENTAL CONCEPTS IN ANALYTICAL PROCEDURES Flashcards
- Facilitate the correct performance of analytic procedures that yield accurate and precise information, aiding patient diagnosis and treatment.
Clinical Laboratory
Use to measure the following
scales
Thermometer/ Temperature
Basic unit of temp in SI system
Kelvin (°K) scales
3 Major types of Thermometers:
Liquid-in-glass
Thermistor probe
Digital thermometer
- Uses colored liquid encased in
plastic material with a bulb at one end - Measures temp between 20 and
400° C
Liquid-in-glass
Accurate and fast-reading
Advantages: SIZE and MILLISECOND RESPONSE
TIME
Thermistor probe
Vessels holding/transferring
liquid are designed either to
contain or deliver a specified
volume.
Laboratory Vessels
Borosilicate
Kimax/Pyrex
Aluminosilicate
Corex
Acid and alkali resistant
Vycor
Plasticware
- Polysterene
- Polyethylene
- Polypropylene
- Tygon
- Teflon
- Polycarbonate
- Polyvinyl chloride
Designed to hold different volumes rather than one exact amount.
Erlenmeyer flasks and Griffin
beakers
Are glass utensils used to transfer liquids.
Pipettes
DESIGN
To Contain
To Deliver
Do not deliver the exact/same amount
To Contain
- Referred as “rinse-out pipettes”
- Calibration medium - mercury
- Ex. WBC Pipet , SAHLI Pipet, RBC
Pipet
To Contain
Delivers the exact/same amount
To Deliver
- Designed to drain by gravity
- Calibration medium - Distilled
water - Ex. Serological Pipet, volumetric,
Mohr pipet (the tip should remain in contact with the side of the vessel for several seconds after the liquid has drained.)
To Deliver
Drainage Characteristics
Blowout
Self-draining
- Has “etched rings” located near the top of the pipet
- The last drop should be blown out to obtain the exact volume of liquid.
Blowout
- No etched rings
- Designed to drain liquid by gravity
- The tip of the pipet should not be in contact with accumulating fluid in the receiving vessel during
drainage.
Self-draining