EK B2 Reproduction system and development COPY Flashcards
Mitosis
Mitosis is a clonal division (means two cells identical to parent, two daughter cells diplioid how we replicate somatic tissue most of tissue of body except for egg adn sperm cells)
Produces two diploid cells identical to the diploid parent
Used for cell replication in somatic tissues
Two mechanisms for passing down genetic information:
mitosis and meiosis
meiosis
Meiosis is a reductional division- means start with diploid get haploid
Produces four haploid gametes from a diploid parent
Takes place only in the gonad ONLY TAKES PLACE HERE
Process for producing egg and sperm cells
Fusion of haploid gametes during fertilization reconstitutes diploid zygote organism** which allows offspring to be diploid again
Cell cycle
- Cells move through a cell cycle
- Four phases of the cell cycle: G1, S, G2, M
- First three phases are “interphase”
- S phase is synthesis, when DNA replication occurs
- During G1 and G2 phases, cell growth and protein synthesis
- M phase is mitosis
- Non-dividing cells (e.g., neurons) are permanently arrested in G0 phase (before S)
- Loss of cell cycle control can lead to cancer
diploid
2n= 46 chromosomes in humans
n= 23 pairs** but if took a skin cell it would have 2 x n= 46 chromosomes two copies of chromosome 1, two copies of chromosome 2 and 2 copies of chromosome 3….
haploid
n= 23
- chromosome 23 is X in a female always, and X or Y in a male*
- NO PAIRS
pairs 1-22 chromosomes
autosomes
pair 23 chromsome
sex chromosome
female: XX
male: XY
so 23 is XX or XY
cell cycle 2
- a sliver of cell cycle is mitosis, but then the whole rest of this is interphase, everything except for Mitosis, M section*
- interphase broken up into: G1, S, G2
- G1= cell growth
- S= stands for DNA synthesis which is the same as replication* when doubling amount of dna in preparation for cell division*
- G2= another growth period
- M= actual division Mitosis
G0
- not all cells dividing all the time, some in dormant phase called G0 offramp called G0
- this is like neurons* for exampe are not actively dividng they are at rest in G0 so that is a way that they can not be actively going around and around the cell cycle
- cells can go into G0 for a little while and then go back out again, but neurons are permanetly in G0
- they are considered postmitotic cell not going around cell cycle and dividing, why neuron damage is os bad no cell cycle to create new cells easily
- not all cells divide with same frequency= NEURONS DO NOT DIVIDE* but then have cells that get a ot of wear and tear adn dividing a lot liek skin cels dividing all the time, lining of our GI tract food passing through those cells divide a lot so epithelial linings internal or external on surface of body tend ot be rapidly dividing or consistently dividing*
who does cell cycle the most
- skin cells, GI inner cells/lining of GI tract
- BUT THE MOST IS CANCER** this the key underpining for why they are so deadly
- very rapidly dividing but unregulated BAD* cell division not normal its bad*
- neurons- non dividing
centrioles, centrosomes and mitoic spindle
Each cell has a centrosome
Centrosome duplicates in S phase (now two)
In mitosis, the centrosomes organize microtubules and the spindle
Kinetochore microtubules attach to kinetochores of chromosomes
Polar microtubules overlap with each other (do not attach to kinetochores)
Aster microtubules help support spindle
homologous chromosomes vs. sister chromatid
Homologous chromosomes are non-identical maternal and paternal copies of a pair
Sister chromatids are identical chromosome copies from DNA replication
Sister chromatids are linked at their centromere
mitosis card 2
5 phases of mitosis
• Interphase: DNA has already replicated, but chromatin is decondensed
• Prophase: nucleus breaks down, chromosomes condense
• Metaphase: chromosomes align on metaphase plate
• Anaphase: sister chromatids separate and are pulled towards poles
• Telophase: chromosomes decondense, cytokinesis divides cytoplasm to daughter cells
Motor proteins are critical for chromosome separation
Kinetochore microtubules lose tubulin subunits, pulling chromosomes to poles
Polar microtubules push against each other, pushing the spindle ends apart
metaphase
- chromosomes align on metaphase plate
- chromosomes line up single file on metaphsae plate, 2n=4
- get daughter cells when pulled apart with 4 each, 2n each* and it will have all the same alleles so genetically identical to original diploid cell that entered into mitosis
sister chromatids
- daughter cells right at end of mitosis do not have same amoutn of dna as parent cell in metaphase
- sister chromatids are two lines together, genetically identifcal to each other all the same alleles*
- in mitosis sister chromatids separate, anaphase they pull apart, what separates during anaphase its the sister chromatids*
- if look at daughter cells in order for them to undergo mitosis again and make 2 daugher cells of its own has to go around cell cycle, duurign S phase whole deal with replication is go from one chromosome to sister chromatids, so replication doubles the amount of DNA but does not change the numebr of chromosoems***
- BOTH 1 CHROMOSOME, middle point on chromosome called centromere** so formal way of saying this to count numebr of chromosomes ocunt numebr of centromeres**** if think abotu X like picture with sister chromatids one centromere, one belly button, not total amount of DNA number of centromeres that defines number of chromosoems*
centrosomes
those are microtubule organizing centers for the cell*
questions on mcat- think about centrosome, these spindles made of microtubules, microtubule organziing centers are centrosome, command center for microtubules spindles that have to attach to centromeres of each of these chromosomes
if cell needs to organize microtubules for some other purpose, cell can also make use of centrosome, which part of the cell is in charge of organzing other microtubules for some othe rprocess they tell you about, if process governing where cells beign set up that is the centrosome* even if not talkign about mitosis some other thing cel using microtubules for*
centrioles 1
- centrioles help centrosomes do their job
- animals do have centrioles* centrosomes contain centrioles
- centrosome is organizing microtubules**
centromere
each chromosome has 1 centromere the belly button, how we count dna**
like glycolysis, etc names words sound the same and come up in mc questions
Interphase:
Interphase: DNA has already replicated, but chromatin is decondensed
Prophase:
Prophase: nucleus breaks down, chromosomes condense
Anaphase
Anaphase: sister chromatids separate and are pulled towards poles
meiosis part 1
Meiosis is a reductional division
One diploid cell → two cell divisions → four haploid gametes
DNA replication before meiosis I
Prophase I: nucleus breaks down, chromosomes condense
Homologous chromosomes synapse (form synaptonemal complex)
Recombination (crossing over) occurs
Metaphase I: tetrads line up on metaphase plate, held together by crossovers
Anaphase I: crossovers are resolved, homologous chromosomes separate
Telophase I: daughter cells form and cytokinesis takes place
• Sister chromatids are still joined
prophase 1
meiosis
nucleus breaks down, chromosomes condense
Homologous chromosomes synapse (form synaptonemal complex)
Recombination (crossing over) occurs
where you get crossing over*** the mechanism for getting recombination in gametes the time when that is occuring is prophase 1*
if cell is 2n, total of 4 chromosomes, in mitosis ignore fat chromosomes exist as pairs, now pairs find each other; say these are the two copies of chromosome 1, same genes but not necessarily same alleles, when crossing over occurs allele for blue eyes swapping over with allele for brown eyes* still gene for eye what defines pairs, genes for eye color here is the gene for ear wax etc
tetrad
- what is important in Prophase 1= still total 4 chromosomes, pair members are finding eachother and form a temporary unit called tetrad to enable crossing over
- tetrad= homologous chromosomes come together
- these are the two copies of pair number 1 and two copies of pair number 2 find each other
- 4 total chromosomes, before tetrads were formed
Metaphase I (meiosis I)
Metaphase I: tetrads line up on metaphase plate, held together by crossovers
DO NOT LINE UP SINGLE FILE, homologous pairs line up together*
Anaphase I meiosis I
Anaphase I: crossovers are resolved, homologous chromosomes separate
PAIRS SEPARATE*
get 2 haploid daugher cells, already genetically different than parent cell becuase parent cell was diploid*
Telophase I meiosis I
-daughter cells form and cytokinesis takes place
• Sister chromatids are still joined
this is in meiosis I*
meiosis II
easy just like mitosis*
n daughters have half amount of dna because sister chromatid have separated but same number of chromosomes*
Prophase II: nucleus breaks down, chromosomes condense
Metaphase II: chromosomes line up on metaphase plate
Anaphase II: sister chromatids separate
Telophase II: nuclei reform, cytokinesis proceeds
• Products are four haploid cells (with recombined chromosomes)
Prophase II
Prophase II: nucleus breaks down, chromosomes condense
non-disjunction
- abnormal separation* problems with separation**
- can occur in meiosis I or in meiosis II, question asked before which is more serious? which leads to more abnormal gametes*
- not always sticking together forever, but for too long so when meiosis is finished those two ended up on one side and then the other pair separated normally*
- can get ultimately n+1, n-1 n-1, n+1
- EARLIER in meiosis I is more serious, effects closer to trunk of tree all branches get effected!!
- when nondisjunction happens in meiosis II only effects half progeny daughter cells*
Metaphase II
Metaphase II: chromosomes line up on metaphase plate
Anaphase II
Anaphase II: sister chromatids separate
Telophase II
Telophase II: nuclei reform, cytokinesis proceeds
• Products are four haploid cells (with recombined chromosomes)
meiotic defects and nondisjunction 2
In non-disjunction, errors occur in chromosome segregation
Failure of chromosome separation in meiosis I or II yields abnormal gametes
One gamete lacks chromosome, other has extra chromosome
Results in trisomy or monosomy after fertilization
Almost all trisomies and monosomies are lethal
Down’s = trisomy 21
Kleinfelter’s = XXY
Turner’s = XO
image nondisjunction in meiosis I
image of nondisjunction in meiosis II
trisomy and monosomy
normal fertilization=n (egg) + n (sperm) –> 2n zygote
abnormal case: n+1 (eggs) + n (sperm) –> 2n + 1 is called trisomy, one pair has an extra chromosome
n-1 (egg) + n (sperm)–> 2n-1 monosomy
any abnormal cases when do not get n for zygote, are trisomy and monosomy***
you can have trisomy or monosomy in an egg and sperm! so sperm can also have n+1
aneuploidy
any abnormal cases when do not get n for zygote, are trisomy and monosomy***
vast majority of these pregancies do not survive** reflects something seriously wrong with embryo**
some trisomies and monosomies that are actually less serious
-if you have a trisomy of chromosome 1 not physically possible to walk this earth, can get trisomoy with chromosome 21 which is down syndrome, not liek chormosome 1 undergoes nondisjunction more than any other chromosome its actually a milder phenotype can survive with trisomy 21 why have that condition, nothing special about chromosome 21 that makes it stick to iteslf more it just can survive*
sex chromosomes disjunction/ trisomy
Kleinfelter’s = XXY- very mild, a lot of ppl with that do not know they have it, can cause problems with fertility* but up until that poitn can look like a male never known*
Turner’s = XO- turner’s present as female, not deadly causes problems with fertility or cardiac issues but definitely something that ppl live with*
can have trisomy/monosomy at sex chromosomes and survive; normally someone who has Y chromosome have male phenotype
Down’s
= trisomy 21
reproductive system
- Fusion of haploid gametes → fertilization
- In humans, fertilization occurs internally
male anatomy
sperm cells created in testis, meiosis in there
- epididymis immature sperm cells mature, in men the sperm cells mature and gain their tails allowing them to swim, but maturation process happens in epididymis
- so they are stored and mature in epididymis
- during ejactulation, sperm cells go up around called vas defens
- if you following up and around, then tube goes down and out urethra* same as in men, same tube carries sperm and urine*
know on image vas deferens, testis, epididymis so vas deferens then to urethra and out*
3 accessory glands in male anatomy-
know seminal vesicle, prostate gland, bulbourethral gland
3 accessory glands in male anatomy 2
Seminal vesicles add fructose and prostaglandins
Prostate gland adds alkaline secretion
Bulbourethral gland adds mucus lubricant
know along the way as sperm is traveling up around through vas deferens, three glands that add secretions! 3 accessory glands add secretions to sperm so sperm cells plus secretions make semen
spermatogenesis 1
meiosis starts at primary spermatocyte
after meiosis I have 2 secondary spermatocytes, after meiosis II you have 4 spermatids* those mature into 4 spermatoza (sperm cells) the mature sperm cells*
Sperm are generated throughout adulthood
Spermatogonium → 1° spermatocyte → two 2° spermatocytes → 4 spermatids → 4 spermatozoa
occurs inside seminiferous tubules* other cells called sertoli cells which are also present and support cells, shown ind iagram in purple, if looking inside of seminiferous tubule inside testy see cells in different stages of meiosis doing there thing, so see primary and secondary then also these sertoli cells providing additional support
sertoli cells
Sertoli cells nurse developing spermatids
Spermatids mature into spermatozoa with tails
structure of sperm cell
- sperm tail flagella made of micotrubules, n in picture where nucleus and DNA are
- brownish part area called midpiece where there is a ton of mitochondria, again because sperm cells need to swim so need to be able to make lots of atp
- Sperm has head, midpiece, and flagellum tail
- Head contains sperm nucleus
- Midpiece contains coiled mitochondria
- Flagellum is composed of 9+2 microtubule structure
- Acrosome at sperm tip has enzymes for egg penetration=acrosome enzymes for penetrating egg at top of sperm cell
male sexual hormones
GnRH secretion from hypothalamus begins prior to puberty, continues in adult
GnRH → stimulates anterior pituitary → FSH and LH
FSH → stimulates Sertoli cells and spermatogenesis
LH → stimulates interstitial (Leydig) cells → produce testosterone
Testosterone is the main androgen
Testosterone needed for spermatogenesis
Testosterone needed for secondary sex characteristics (bone, muscle, hair, voice)
Testosterone deprivation → sterility
Negative feedback loop: testosterone inhibits GnRH and LH secretion
female sex anatomy
Female gonads are the ovaries, gametes are eggs (ova)
Ova are released into oviduct (fallopian tube)
Fertilization of egg occurs in oviduct
Blastocyst implants in uterus wall
Uterus epithelium = endometrium (lining of the uterus)
If no fertilization, endometrium is sloughed off = menstruation
Vagina → cervix → uterus
bottom of uterus is cervix* fertilizaton occurs sperm has to swim all the way up through vagina, through uterus and then fertilization occurs in oviduct
spermatogenesis 2
Spermatogonium → 1° spermatocyte → two 2° spermatocytes → 4 spermatids → 4 spermatozoa
Spermatogonium is diploid progenitor cell
Spermatogonium differentiates into primary spermatocye
Primary spermatocyte is diploid and enters meiosis
Meiosis I produces two secondary spermatocytes
Meiosis II produces two haploid spermatids from each secondary spermatocyte
Primary spermatocyte → four spermatids
Sertoli cells nurse developing spermatids
Spermatids mature into spermatozoa with tails
Maturation occurs in epididymis
During maturation sperm loses most cytoplasm
LH
stimulates production of testosterone, obviously if testosterone is high does negative feedback on brain on hypothalamus and anterior pitutitary
LH → stimulates interstitial (Leydig) cells → produce testosterone
receptor on testes
FSH
stimulates spermatogenesis*
FSH → stimulates Sertoli cells and spermatogenesis, hormone that mostly aggressively binds to testes and do spermatogenesis, there are receptors for FSH and LH on the testes those are both protein hormones** testosteorne is a steroid*
testosterone
- main androgen= which is a category of steroid hormones* think about testosterone and androgen being basically synonyms for purpose of mcat, obviously hormones really really similar to testosterone with small chemical modifications form family of androgens
- Testosterone needed for spermatogenesis, need it to do the spermatogenesis*
- Testosterone needed for secondary sex characteristics (bone, muscle, hair, voice) all changes that happen in puberty
- Testosterone deprivation → sterility
- Negative feedback loop: testosterone inhibits GnRH and LH secretion
GnRH
GnRH secretion from hypothalamus begins prior to puberty, continues in adult
GnRH → stimulates anterior pituitary → FSH and LH
hypothalamus uses different hormones to signal to anterior pitutiary** so its just the thing secreted by hypothamalus to atnterior pitutiary
Gn releasing hormone, telling anterior pituitary to release FSH and LH
male hormone feedback loop
fertilization progress
Vagina → cervix → uterus
if fertilizaiton occurs embryo will implant in lining of uterus* fertilization in oviduct if fertilizatin occurs most of the time it doesnt occur, but if it does it happens in the oviduct, 5-6 days later have an early embyro that would implant in wall of uterus in endometrium linng
from a medical point of view embryo implanting in endometrium lining from religious points of view it all begins at fertilizaton= big difference if you have a form, intervention that prevents implantation* from a medical point of view most doctors would call that birth control but some catholic people would call that a form of abortion interfering with life after life has begun* if it all begins when fertilization occurs then everything after that is absorption, but from a medical poitn of view pregnancy begins at implantation*