EK B2 Ch6 Musculoskeletal system

Question 1

Bone marrow

Answer 1

where stem cells are born

Question 2

bone

Answer 2

think of it as living tissue interacting with other phsyiological systems, responding to hormones dynamic rather than scaffolding inside the body that is static!

Question 3

skeleton anatomy 1

Answer 3

• Vertebrate skeleton has two divisions
• Axial skeleton: skull, vertebral column, ribs, sternum
• Appendicular skeleton: bones in limbs, shoulders (pectoral girdle), hips (pelvic girdle)

Question 4

skeleton anatomy 2

Answer 4

• Vertebral column has vertebrae, sacrum, and coccyx
• Vertebrae are cervical (neck), thoracic (chest), lumbar (back) and sacral (pelvic)
• Ribcage has 12 ribs, most fused to sternum, a few attached to cartilage or free floating

Question 5

Gross bone structure

Answer 5

• Periosteum is membrane of connective tissue that covers bone
• Compact bone is outer layer, hard and dense
• Spongy bone is inner layer, more porous and less dense
• Diaphysis is long shaft portion of bone
• Epiphysis is bulbous end of bone
• Long bones contain marrow
• Yellow marrow is fatty tissue (in hollow of bone)
• Red marrow is source of blood cells (in spongy bone)

Question 6

metaphysis and epiphysical line

Answer 6

• Metaphysis is cartilaginous growth zone, between diaphysis and epiphysis (in children) transition area, caritlage converted to bone when long bones grow, how kids get taller cartiaginous region where more bone cells get added! SO IT is sometimes referred to as the growth plate!

There is a line where metaphysis meets epiphysis labeled epiphysis line which shows how far bone growth has gotten, doctor can tell how much more likely they will grow -position of epiphysisal line gives read on how much cartiglate has been pushed into bone

Question 7

compact bone vs spongy bone

Answer 7

all around parameter of diaphysis is compact bone, hard bone which is really what is responsible for anomotical support, hard part marrow is not compact bone inside of the compact bone surroundings spongy bone, called spongy because pourus so a lot of holes in it, not as strong

Question 8

Bone marrow in center…

Answer 8

Bone marrow all through center of bone

red bone marrow gives rise to blood cells

yellow marrow which is fatty deposits, so a lot of fat in bone

Question 9

periosteum

Answer 9

thin membrane around outside of bone called periosteum

Question 10

cartilage

Answer 10

• Cartilage is an elastic connective tissue, provides joint cushioning
• Cartilage contains chrondrocyte cells and extracellular collagen
• Embryonic skeleton is cartilaginous, becomes bone (ossification)- process still going on in long bones when kid is growing, happens early in skull, carilaginous skeleton gives way to skeleton, but in long bones to get kid to keep going remains carilaginous until bone growth is finished
• Metaphysis is cartilaginous, permits bone elongation
• Adult cartilage in ear, nose, ribcage, between spinal vertebrae, and in joints as fully grown adults have bits of cartilage in ears, verebtrates other joints because it provides really good cushioning* really really painful when two bones rub directly together in a joint so having cartilage there to cushion that keeps it comfortable for people

Question 11

what kind of cells make up cartilage

Answer 11

chrondrocyte cells

Question 12

osteon

Answer 12

= Haversion system what looks like if have cross section osteocytes are bone cells

canal in middle of one of these systems called Haversion canal where blood vessels and nerves are found, the osteocytes bone is hard as it grows and gets stronger it creates this hard extracellular matrix around itself, but osteocytes are living cells in bone, problem for evolution to solve to keep these cells alive in bone even when building fortress around themselves so bone would be strong, system of canals and little passages haversian canal is where blood vessels are, like Venice, so oxygen nutrients move out through that canal system diffuse out and reach osteocyte, then carbon dioxide and waste go back through center and enter blood supply, without it osteocytes would not be able to stay alive

Question 13

Osteoblasts

Answer 13

Osteoblasts build up bone (ossification) BLASTS BUILD BONE UP

Question 14

Osteoclasts

Answer 14

Osteoclasts break down bone (resorption) BREAK DOWN BONE. CHEW BONE UP *not to be confused with reabsoprtion

Question 15

Bone remolding

Answer 15

Bone constantly beign adjusted, broken down or built up, can change shape, its an active tissue, as bone is developing osteoblasts, the bone building cells are what is really active

Osteoblasts secrete collagen* collagen forms a web on that web there are these crystals called hydroxy appetite crystals that get deposited on the collagen that makes bone hard and what gives bone its strength, made of calcium, phosphate and hydroxide ions*

Question 16

crystals deposited on collagen

Answer 16

hydroxyapatite, but if unpack what is in a hydroxyapatiate crystal made of= it is made of calcium, phosphate and hydroxide ions to make hard crystal structure what gives bone its strength

Question 17

osteocytes

Answer 17

trapped in extracellular matrix with hydroxyapatite crystals, older cells so need whole osteon system set up to nurish those cells!

Osteocytes are the longest living bone cell, making up 90–95% of cells in bone tissue in contrast to osteoclasts and osteoblasts making up ~5% (40). Osteocytes form when osteoblasts become buried in the mineral matrix of bone and develop distinct features.

Question 18

Hyperactivity of osteoclasts →

Answer 18

Hyperactivity of osteoclasts → osteoporosis, which is when bone gets fragile and brittle and broken down, if osteoclasts are too active can weaken bone breaking down bone and making it less strong

Question 19

parathyroid hormone and calcitonin

Answer 19

inc amount of calcium in blood

calcitonin dec amount of calcium in blood

so parathyroid hormone stimulates osteoclasts so if trying to release calcium from bone by breaking down bone, cells stimulated are osteoclasts break down bone

ex why bones broken down ballerinas not enough calcium, on flip side calcitonin trying to reduce amoutn of calcium in blood will stimulate other kind of cells to build bone, after age of 30 don’t build bone* so after that having enough calcium is about maintaining bone mass, which will then be there for a lifetime

Question 20

bone mass and aging

Answer 20

• Bone mass is gained only until age 30
• After age 30, bone loss exceeds bone formation
• Very low bone mass → osteoporosis
• Postmenopausal women more susceptible to osteoporosis after menopasue when women have less estrogen much more susceptible to osteoporosis, why falls and hip fracture are such a big concern when taking care of elderly especially elderly women

Question 21

Tendon

Answer 21

Tendon attaches muscle to bone

THINK:

“Tara must be a bully” tendons connect muscle to bone

Question 22

joints are classified by

Answer 22

how much joints can move relative to each other

skull is a good example of that, we don’t want bones of cell skipping around, called immovable or fibrous joint btw bones

slightly movable joints- best example verbrate, move around a bit to absorb shock, fact can move a bit helps absorb shock

have the joint that can move the most bones can move the most, called free movable or synovial joints

Question 23

synovial fluid

Answer 23

Freely movable/synovial joints = full movement (e.g., knee, elbow, shoulder)

Synovial joints have capsule with synovial fluid, are covered by connective tissue

can get infections, tares in ligaments connecting two bones at the knee, tons of conditions that involve these joints like knee or shoulder

cartilage critical in joints

Question 24

muscles in the body

Answer 24

muscles in body come in pairs! one has to relax one has to work, agonist produces movement, antagonist one that if it contracted it would cause the opposite movement!

Question 25

muscle class

Answer 25

• Abductor moves away from the midline (lifting arms, or moving away fingers)

“I have to move away from my diet to get abs”

• Adductor moves towards the midline (adding to center)

Question 26

muscle

Answer 26

• Muscle generates force and permits movement
• Muscle contraction squeezes vessels, aiding in blood circulation and lymph movement
• Muscle contraction important for thermoregulation (shivering releases heat)
• All muscle contraction depends on Ca2+ skeletal muscle plays a really big role with circulatory system, keep blood moving through veins through action of skeletal muscle, need skeletal muscle movement to keep circulation normal, muscle contraction also important for thermoregulation, when get cold use our muscles to shiver and produce heat** in everything we say about muscle contraction Calcium plays a starring role, different muscle cells but all muscle cells involve calcium***

Question 27

skeletal muscle

Answer 27

Skeletal is striated

voluntary

multinucleate

innervated by somatic nervous system

striated= means looks like it has stripes

voluntary= so we can decide to contract our skeletal muscle or we can decide to run or lift a cup or whatever

multinucelated= meaning really striking when look at skeletal muscle cells, sooo weird have 20 nuclei look like row of eyeballs but normal for skeletal muscle cells

innervated by somatic nervous system, called motor system or the somatic system, the nerves that give instructions for skeletal structures, these are the nerves somatic neurons that axons extend a great distance from spinal cord to big toe synapse with acetylcholine there is a neuromuscular junction there between neuron and skeletal muscle cell, when acetylcholine binds to its receptor in skeletal muscle cell the muscle cell contracts

Question 28

Cardiac

Answer 28

cardiac muscle cell is striated, involuntary, 1 or 2 nuclei, innervated by autonomic nervous system

• 1 or 2 nuclei v important**
• Contracts and relaxes rhythmically, coordinated by a pacemaker
• Cardiac muscle fibers/cells are electrically coupled by gap junctions with structure called intercalated discs in thin heart muscle need free flow of ions from one to the next to get the whole heart to contract all together come back to this in circulatory chapter this is just a little preview
• Intercalated discs are physical and electrical junctions between muscle fibers
• Cardiac muscle fibers are branched, in a mesh
• Innervated by autonomic nervous system, which can influence contraction rate/strength
• Like skeletal muscle, cardiac muscle is striated, but unlike skeletal muscle, cardiac muscle cannot be consciously controlled and is called involuntary muscle. It has one nucleus per cell, is branched, and is distinguished by the presence of intercalated disks.

Question 29

smooth muscle

Answer 29

• Smooth muscle is not striated, is involuntary
• Organization of muscle is less regular
• One nucleus per cell
• GI tract, blood vessels, arteries, bladder
• Smooth muscle fibers may be connected by gap junctions
• Contracts slowly and contraction is sustained
• Innervated by autonomic nervous system, meaning automatic not voluntary

Question 30

Neurogenic =

Answer 30

Neurogenic = nerve impulse generates muscle contraction

-muscle cells must have an input from nervous system to contract

Question 31

Myogenic =

Answer 31

• Myogenic = muscle generates its own contraction, able to generate own contractions, in petri dish and watch it beat cool and weird, can still be influenced by nervous system, have ability to contract on own but nervous system can tell them to contract faster or slower, still controlled by nervous system in ways that are really important, so skeletal muscle is neurogenic, it has to have input from nervous system, acetylcholine, nervous muscular junction

cardiac and smooth muscle are myogenic but nervous system still plays a role

Question 32

When sarcometer contracts what changes?

Answer 32

H and I change, ones that spell Hi go away

Question 33

SR

Answer 33

stores a lot of calcium, lot in SR, as action potentials go down through its tubules they trigger voltage gated calcium channels on the membrane of the SR

• so when the action potential gets there calcium channels open in the membrane of the SR, and we said that the Ca concentration in SR is much greater and Ca concentration is lower in cytoplasm, means calcium is going to go out into cytoplasm, also said calcium is crucial for muscle contractions

MOVES DOWN ITS CONCENTRATON GRADIENT*

Calcium greater in SR and less in cytoplasm, so going down gradient!!! Why want this!

Question 34

Synaptic terminal of motor neuron

Answer 34

acetylcholine binds to muscle cell, get action potentials, action potentals go deeper into cell cause calcium to be released into cytoplasm

Question 35

head of myosin

Answer 35

The head of myosin has to bind to actin, don’t want myosin binding all the time or contracting all the time, see how it is regulated, if look closely on lefthand side of figure, if you see how look at orange beads of actin and grey rope wound around them, little holes in beads being covered up by grey rope –>what that is showing is that the little holes represent binding sites where myosin can attach to actin

Grey rope is a protein called tropomyosin

And at rest tropomyosin is covering the binding sites where myosin would bind to actin, and then there are also these purple proteins called troposin

Question 36

Role of Ca2+ in muscle contraction

Answer 36

binds to troponin, troponin protein changes shape, pushes tryopomosin out of the way and exposes the sites on actin where myosin can bind

point of diagram when calcium binds to purple tropoin protein causes this shift so actin grey stuff moves and myosin can actually bind to actin*

trypomosin= grey rope

protein troponin= purple thing, Ca2+ ions bind to troponin protein on actin filament

Question 37

power stroke cycle

Answer 37

trying to understand how action potential, signal from neuron causing action potential makes sacromere go in and shorten how myosin binding to actin can pull actin so more overlap between the two kinds of protein and that is what this image is about

calcium makes actin available to be bound to myosin but actual contraction is powered by ATP*** how exactly does ATP cause actin and myosin to move, kinda of weird mechanism

1. myosin head low energy confirmation bound by ATP, not hydrolyzed or used yet
2. ATP is hydrolyzed to ADP and Pi. The potential energy from ATP is transferred to myosin head, so what we are suppose to see is that the myosin head is in a different position in its high energy confirmation
3. when myosin is in its high energy confirmation across bridge forms, so that just means myosin actually binds to actin
4. ADP and phosphate group are released, potential energy stored in myosin is being used or converted to kinetic energy to push the actin push the thin filament moves toward center of sacromere
5. Then returns to low energy confirmation, attached to actin takes ATP coming in in step five to release the myosin from the actin, ATP needed to detach from actin, pushing actin more and more but every time and cycle requires another ATP

Question 38

why need atp for myosin….

Answer 38

idea need atp for myosin to let go of actin helps explain rigermortis, bodies stifen after they die is becuase not producing atp anymore and so all through the body myosin gets stuck to actin in a rigid state*

Question 39

power stroke cycle 2

Answer 39

• if do muscle contraction and then really relax way to stop this is to pump calcium out of cytoplasm
• another thing ATP is important for in muscle cells is to sequester and pump ATP back into sacroplasmic reticulm, then if you remember that mechanism with tropomyosin, if calcium is not present then the binding sites on actin for myosin will get covered up again* so then myosin cannot bind to actin and muscle contraction cannot occur* how we would relax at the end of all of this

Question 40

muscles, atp and energy

Answer 40

Muscle needs lots of ATP and is rich in mitochondria

• Rigor mortis is ATP depletion, can’t release myosin cross bridges
• Second storage compound is creatine phosphate
• Creatine phosphate + ADP → creatine + ATP
• Third storage compound is glycogen → breakdown to glucose → ATP
• Repeated contraction causes O2 debt and ATP depletion
• During O2 debt, anaerobic glycolysis produces lactic acid

Question 41

Creatine phosphate

Answer 41

Create a little of atp through substrate level phospholraytion

More creatine in muscle cells can make more atp

Muscle cells can make some small additional amount og ATP from creatine phosphate and that gives them extra atp, another way can boost their atp ability*

Question 42

when exercising hard

Answer 42

can do anerobic respiration and fermentation, causes lactic acid to build up in cells what is the muscle burn you get when exercising really hard

Question 43

temperature and muscles

Answer 43

when muscles contract a lot of atp energy is released as heat, why get pretty hot when exercise but can harness that when cold by shivering

Question 44

tetanus and muscle tone

Answer 44

• Single electrical stimulation of muscle causes a simple twitch
• Summation of twitches provides a smooth contraction
• Smooth, sustained contraction is called a tetanus
• Muscle tone (tonus) occurs from partial contraction of some muscle fibers

Question 45

tonus

Answer 45

muscle tone contraction all the time, just sitting there have some muscle tone to maintain position

Question 46

fast twitch muscles

Answer 46

what sprinters need, rely on anaerobic respiraiton

glycolysis through glycogen

fast twitch muscles do not have as much of a blood supply so look white, lesser blood supply is because function with less oxygen do more anaerobic respiration, innovated by motor neurons with very large diameters, very frequent signals or action potentials can be delivered

Or like body builders*

Question 47

fast twitch muscles 2

Answer 47

great for generating bursts of force but white fibers get tired easily so sprints rather than marathons

Fast-twitch = white muscle

Fast-twitch has few mitochondria and little myoglobin

Fast-twitch gets energy from glycolysis and glycogen

Fast-twitch innervated by motor neurons with large diameter axons

Good for generating lots of force quickly, but fatigues easily

Question 48

slow twist muscles

Answer 48

good for marathon runners, sustained activity, want slow twitch muscles, do everything to have more slow twitch muscle, if do a different kind of exercise, long distance runners have to do everything to have as much red fibers as they can have

Slow-twitch = red muscle

Slow-twitch has lots of mitochondria, myoglobin, well vascularized

Slow-twitch gets energy from aerobic respiration

Slow-twitch innervated by motor neurons with small diameter axons

Good for long-term aerobic exertion

Question 49

skin

Answer 49

• Skin is a multifunctional organ
• Acts as a protective covering from microbes, injury, UV light
• Provides sensory reception (e.g., touch, temperature)
• Relatively impermeable to water
• Key for thermoregulation
• Skin has excretory function via sweating (water, salts, urea) how can cool off by sweating
• Hair, nails, oil glands, milk glands derive from skin

Question 50

Callus

Answer 50

Callus = area of skin thickened in response to irritation

Question 51

skin layers, epidermis

Answer 51

• Three major skin layers: epidermis, dermis, and hypodermis/subcutaneous
• Epidermis is outermost layer of skin, has 5 layers or “strata”
• Stratum corneum is top protective layer of epidermis (dead cells) top layer
• Stratum basale is deepest layer of epidermis (dividing cells) bottom layer, lowest level of epidermis is where we have stem cells dividing and what basically happens is cells divide and differentiate and move up through layers to top of outer layer of epidemis, by the time they reach very top layer of stratum corneum dead and sluff off, so epidermis is constantly regenerating itself cells constantly differentiating and moving up through epidermis

Question 52

epidermis pigmented

Answer 52

• Epidermis is pigmented with melanin, produced by melanocytes -produces diff ethnicities, its role is to absorb uv light which causes dna damage so of course we want to absorb it
• Melanin absorbs UV light, protecting skin

Question 53

Dermis

Answer 53

if get cut and bleed means gone down to dermis where blood vessels are

• Dermis is underneath epidermis
• Dermis contains blood vessels, sweat glands, hair follicles, and collagen, less collagen in dermis causes skin to sag

Question 54

hypodermis

Answer 54

• Subcutaenous/hypodermis is underneath dermis
• Subcutaenous layer is mainly fat, provides insulation, lack of fat here is skin saging as well again

Question 55

skin and thermoregulation

Answer 55

• Skin regulates body temperature through radiation and sweating

• Skin is cooler than the rest of the body***

• Dilated capillaries promote heat loss, if body wants to conserve heat capillaries can constrict, other way to use skin to cool off is sweating

• Constricted capillaries conserve heat

• Sweating is critical to regulate internal body temperature; when sweat released onto surface of skin it evaporates, cooling process
• During high activity, body temperature must be maintained
• Sweat allows additional heat loss through evaporation

Question 56

skin and thermoregulation 2

Answer 56

part of homeostatsis is about keeping body temperature within narrow range, when talking about nervous system really hypothalamus sending directives in terms of what the body needs to do in terms of warming up or cooling down

Question 57

metaphylsis border…

Answer 57

border called growth plate or ephiphyseal line, metaiphyseal plates; bone replaces cartilage at the epiphyseal plate on an xray this spot can tell how much more the kid is going to grow, so represents a border btw place that is still cartilage and bone, if lots of cartilage that hasn’t turned into bone yet kid will still keep growing; if not much left kid is done growing, or getting taller

it is called both also called metaphylsial plate, ephiphyseal line

Question 58

bone resporption means=

Answer 58

MEANS BONE BREAK DOWN**** which of the following would contribute to osteoporosis, a dec in bone density often found in post menopausal women- what osteoclasts do and what parathyroid hormone would inc, the inc bone resportion

Question 59

which of the following decreases when a sarcomere contracts?

Answer 59

• boundaries of sarcomere are Z lines, when sarcomere contracts Z lines get shorter! closer together! -the purple represents the myosin, thick filaments and the orange represents actin the thin filaments; sarcomere contracts when myosin and actin slide relative to each other meaning they overlap with each other more****
• region of overlap inc so how sarcomere as a whole gets smaller, like hands sliding past each other overlapping more, none of your fingers are getting shorter just overlapping more
• the I band is actin with NO OVERLAPPING MYOSIN, so when do have more overlap I band will get smaller*

-A band represents the whole length of the myosin, D just says length of thin filaments don’t get smaller just overlap more with myosin*

-H represents thick with no overlaping thin filaments, so could also say this changes because get NO overlap, so that area dec

Question 60

11. A drug responsible for smooth muscle relaxation would be least likely to cause=

Answer 60

• this turns on where you have smooth muscle!*** so this drug is only going to target smooth muscle, so can definitely cause A. dilation of peripheral blood vessels, because that is smooth muscle, same with b. relaxation of msucles along with gastro-intestinal tract
• c. is skeletal muscle, relief of pain caused by muscoskeletal tension in the forearm
• d. reduced musclar tension in bladder wall, this is also true becuase bladder surrounded by smooth muscle*

Question 61

13. Calcium ions are critical for contraction of:

Answer 61

• do not need all same muscle contractions, exactly what Calcium does for skeletal but need calcium for all three types for how to contract: cardiac, smooth, and skeletal muscle all need calcium
• answer all of the above!

Question 62

Atp’s role** in power stroke

Answer 62

• ATP binds to myosin head before myosin is attached to actin, which allows myosin to release from actin
• atp binds to myosin allows it to let go of actin
• number 1. atp bound to myosin
• 2. second step for ATP to be hydroyzled, energy in ATP gets transfered to myosin head as go from 12 oclock to 3 oclock
• 3. myosin head high energy confirmation, energy from atp now in myosin head*, atp’s job is done in a way
• 4. THEN myosin binds to actin that is the point here, you do not need ATp in the step where myosin binds to actin** cross bridge, energy in myosin head is used to push actin to the left
• 5. 9’ oclock myosin head in low energy configuration, need another ATP to release myosin from actin, then cycle can start over!
• myosin pushes actin then lets go, then pushes and lets go happens many many times in course of single muscle contraction* lots of actions of myosin to make shortening of sarcomere and therefore shortening of the whole muscle*

Question 63

anatomy of long bone image

Answer 63

Question 64

voluntary muscle ex.

Answer 64

skeletal muscle, like cerebral cortex

spinal cord also contributes to voluntary control of muscles, remember if something controlled by me* if I control the muscle function then I will use either my cortex or my cord which happens to start with a C*

so controled and voluntary= cortex or cord comes in handy*

Question 65

involuntary control ex.

Answer 65

cardiac and smooth muscle

stuff going on beyond me, beyond my self control, if beyond me will have to use either brain stem or neuronal tissue that sits besides spinal cord to cause involuntary contraction of muscle*

Question 66

myosin steps

Answer 66

• myosin uses atp to crawl around/down actin rope to create ATP energy
• step 1= 1. atp binds to myosin head, 2. myosin releases actin** so that is what happens it touches actin, atp comes and it gets released! so in the next step looks to have released* ATP attached to it still* now on ext step 3. atp hydrolyzes phosphate gets pulled off of it*
• step 2= atp goes to ADP + Pi phosphate group, releases energy to cock myosin protein into a high energy state* so step 2 this thing gets hydrolyzed releases energy, know atp is the energy currency of biolgoical systems* can really imagine it as changing confirmation spring loads that protein to go into a step to crawl along the myosin, so step 2 plus energy, then can say it cocks the myosin protein or enzyme to high energy* can imagine it whinds the spring or loads the spring to high energy confirmation* high energy confirmaton of protein just means shape so phosphate group still attached but gets detached from the rest of atp to adp so changes confirmation with energy protein in new shape

Question 67

myosin steps part 2

Answer 67

• 1. atp bids to myosin “head” –> myosin releases actin. end of step one causes myosin proteint o get released, then at end of step 1 naturall step 2 hydrolzyes to atp phosphate releases enrgy allows myosin protein to get cocked at high energy protein next run of actin filament
• so at end of step 2 “cocks” the myosin protein to high energy conformation* now when that phosphate group releases* so when started just sitting here
• step 3- phosphate group released from myosin, releases energy of cocked position, causing myosin protein to push on the actin, its the power stroke in enegine the mechanical movement, original release atp to adp in phosphate puts in spring loaded position, when phosphate releases it it releases the spring* what that does is it pushes on the actin filament* pushes on actin, if imagine myosin fixed then whatever actin it is attached to moves to the right, where get fundamental muscle reaciton
• step 4. ATP released and then we are exactly where we were before did step 1, just one rung furhter to the left on actin molecule*

Question 68

where is tropomyosin

Answer 68

• so tropomyosin protein coils around actin*
• important thing about it coiled around it and attached to actin by another protein called troponin*
• when a muscle is not contracting, turns out tropomyosin is blocking myosin still an area of research not 100% clear tropomyosin blocks myosin to attach to actin where it normally attaches, or sometimes it is attached to actin but stops it from releasing and sliding, so bottom line is tropomyosin blocks the myosin head*** from crawling up the actin*** either physically blocking active binding site
• only way to make it unblocked is for troponins to change shape, only way to do that is high calcium ion concentration*** high enough concentration of calcium ions binds to troponin, changes confirmaiton of troponin enough to mvoe confirmation of tropomyosin

Question 69

tropomyosin 2

Answer 69

normally blocks the myosin head

when have a high calcium ion concentration* they bind to troponin* and then the troponin changes their confirmation and moves the tropomyosin out of the way* then these guys can start walking up the actin, or pushing the actin to teh right, however want to view it

• if low calcium ion concetration then calciums get released from troponin, if concetration becomes really low here these guys start to live so troponin goes back to standard confirmation* and that makes the tropomyosin block the myosin again**

Question 70

without calcium…

Answer 70

tropomyosin is blocking hte ability of myosin to attach where it needs to attach and slide up actin

if calcium concentration is high enough they will bond to troponin, nails down tropomyosin wound around actin, when change their confirmation with calcium ions it moves the tropomyosin out of way so myosin can do what it does

building up a way for muscles to contract and how to control muscles contracting if high calcium concentration within cell the muscle will contract!!

if low calcium concetration then these will release, be blocked and muscle will relax again!

Question 71

sarcoplasmic reticulum

Answer 71

• it has calcium ion pumps on membrane
• what these do is they are atpases, meanign use atp to fuel pumps, have atp come in atp attaches to it, calcium ion attaches to it
• when atp hydrolyzes into adp and phosphate group (Pi) changes confirmation of protein and pumps calcium ion in
• so resting muscle has a very high concetration of calcium ions on the inside; when muscle needs to contract calcium ions get dumped out to cytoplasm of cell, can bound to troponin and do everything just talked about- what we care about how does it know when to dump rest of its ions inside cell!
• this area is what the actin filaments and myosin heads and all of the rest of the troponin and tropomyosin, all exposed to environment over there

Question 72

for muscle cells, acetylcholine

Answer 72

1. released into synpase, so receptor on msucle cell get acetylcholine and then allows sodium to flow into muscle cell
2. causes action potential in muscle cell, postive charge high enough to threshold channel triggers next vltage gated channel allowing more sodium to flow in
3. then action potential goes down T tubule, chemical signal goes down next action potential down t tubular connected to sacroplasmic reticulm, get positive enough right around there complex of protein triggers release of calcium!
4. when action potential gets far enough, mystery box triggers open for Ca ions to escape sarcoplasmic reitculum outside into inside of cell, cytoplasm of cell
5. so when muscle relaxes Ca goes into sarcoplasmic reitcum then when it gets signal dumps it back out into cytoplasm
   6.

Question 73

actin

Answer 73

thin filaments

Think: “All actresses try to be thin”

Question 74

Image of role of Ca2+ in cell

Answer 74

TO BIND TO TROPONIN PROTEIN and change shape, pushing tropomyposin out of the way

Question 75

tetanus

Answer 75

• Smooth, sustained contraction is called a tetanus

Question 76

Myosin

Answer 76

Thick filaments

think: going on instagram everyday is a sin, makes you thick aka dumb

sins make you thick, dumb

Question 77

each muscle fiber

Answer 77

is composed of a bundle of myofibrils, which are composed of actin and myosin (the thin and thick filaments)

Question 78

thermoregulation by muscle

Answer 78

Contraction is ~ 40% efficient, rest of ATP energy is released as heat

Involuntary shivering is body producing heat by muscle contraction

Question 79

Two types of muscle fibers

Answer 79

fast-twitch and slow-twitch

Question 80

Calcitonin

Answer 80

Calcitonin decreases blood Ca2+ → stores Ca2+ in bone

Calcitonin promotes bone formation and osteoblast activity

Question 81

Parathyroid hormone

Answer 81

Parathyroid hormone increases blood Ca2+ → releases Ca2+ from bone

Parathyroid hormone promotes bone resorption

Question 82

Bone composition and Haversian system

Answer 82

Bone is composed of cells and a hard matrix

Matrix contains collagen and hydroxyapatite (calcium phosphate)

Haversian systems/osteons are circular units of organization in bone

Haversian canals = microscopic canals that contain capillaries and nerves

Lamellae = concentric matrix layers around Haversian canals

Lacunae = pits, occupied by osteocytes (bone cells)

Canaliculi = canals connecting lacunae and Haversian canal

Question 83

Haversian canals 2

Answer 83

Haversian canals = microscopic canals that contain capillaries and nerves

Question 84

Lamellae

Answer 84

Lamellae = concentric matrix layers around Haversian canals

Question 85

Lacunae

Answer 85

Lacunae = pits, occupied by osteocytes (bone cells)

Question 86

Canaliculi

Answer 86

Canaliculi = canals connecting lacunae and Haversian canal

Question 87

Bone remodeling

Answer 87

Bone is living and is remodeled continuously (turnover)

Critical for repair and Ca2+ regulation

Two key cell types=

1. Osteoblasts build up bone (ossification)
2. Osteoclasts break down bone (resorption)

Osteoblasts secrete collagen, allowing hydroxyapatite deposition → hard matrix

Osteoblasts trap themselves in matrix, become osteocytes

word remodeling stands for resportion and formation!

Bones thicken in response to mechanical stress (e.g., stronger muscles, physical activity)

Question 88

Ligaments

Answer 88

Ligament attaches bone to bone

BBL

Question 89

Joint

Answer 89

Joints are meeting points of bones

Question 90

Immovable/fibrous joints

Answer 90

Immovable/fibrous joints = fused bones in skull

Question 91

Osteoarthritis

Answer 91

Osteoarthritis = cartilage degeneration in joint, joint pain, and inflammation

Question 92

Muscle pairs and joints

Answer 92

Muscles act in pairs (flexor, extensor)

Agonist is muscle that produces the motion

Antagonist is muscle that elicits the opposite motion

Agonist contracts, antagonist relaxes

Example: to bend arm, biceps = agonist, triceps = antagonist

Question 93

z-line

Answer 93

Neighbouring, parallel lines that define a sarcomere.

Question 94

motor units and neuromsuclar junctions

Answer 94

Skeletal muscle is controlled by motor neurons

Motor unit = one motor neuron and the muscle cells it controls (can be 1000s)

Neuromuscular junction = synapse between axon terminal and muscle

Neuromuscular junction = motor end plate

Acetylcholine release from axon causes depolarization of muscle fibers

Depolarization of muscle fibers causes action potential in muscle

Question 95

Skeletal muscle structure

Answer 95

A skeletal muscle is composed of 1000s of elongated cells

One muscle cell (many nuclei) = a muscle fiber

Plasma membrane = sarcolemma

Cytoplasm = sarcoplasm

ER = sarcoplasmic reticulum

T-tubules are tubular invaginations of sarcolemma

T-tubules permit rapid depolarization and action potential propagation

Each cell/fiber contains several myofibrils

A myofibril is an end-to-end assembly of many sarcomeres

The sarcomere is the unit of contraction

Question 96

Sarcomeres 1

Answer 96

Striations in muscle = regularly repeating sarcomeres

Sarcomere has thin actin filaments and thick myosin filaments

Z line at end of sarcomere is region of anchored actin filaments

A, H, I, M bands are within sarcomere

I band = actin only (with no overlap of myosin)

H zone = myosin only (with no overlap of actin)

M band = band in center of H zone, crosslinks and aligns myosin filaments

A band = full length of myosin plus any overlapping actin

Myosin forms cross-bridges with actin molecules in A band

During contraction: I band and H zone shrink

Contraction is based on filaments sliding past each other

Actin alone not getting shorter or myosin alone not getting shorter but they slide so overlap more with eachother

Question 97

I band

Answer 97

I band = actin only (with no overlap of myosin)

The area adjacent to the Z-line, where actin myofilaments are not superimposed by myosin myofilaments.

Question 98

H zone

Answer 98

H zone = myosin only (with no overlap of actin)

The area adjacent to the M-line, where myosin myofilaments are not superimposed by actin myofilaments.

Question 99

M band

Answer 99

M band = band in center of H zone, crosslinks and aligns myosin filaments

The line at the center of a sarcomere to which myosin myofilaments bind.

Question 100

A band =

Answer 100

A band = full length of myosin plus any overlapping actin

The length of a myosin myofilament within a sarcomere.

Question 101

Molecular mechanism of muscle contraction

Answer 101

Motor neuron releases acetylcholine at neuromuscular junction

Acetylcholine binds receptor and causes depolarization of sarcolemma

Action potential travels across membrane and through T-tubules

Depolarization triggers Ca2+ release from sarcoplasmic reticulum via Ca2+ channels

Ca2+ ions bind to troponin protein on actin filament

Troponin protein changes shape, pushes tropomyosin away

Myosin-binding site on actin filament is now exposed

Myosin head (bound with ADP + Pi) binds actin filament

Myosin head releases ADP + Pi and undergoes power stroke

Actin filament is pulled during power stroke

ATP binds myosin head, allowing myosin to detach

ATP is hydrolyzed to ADP + Pi, cocking myosin head

Cycle repeats and muscle contracts

When motor neuron stops firing, acetylcholinesterase degrades free acetylcholine

Ca2+ is pumped back into sarcoplasmic reticulum

Muscle relaxes

Question 102

Sarcomere 2

Answer 102

• many sarcomere contracts at same time, when a sarcomere gets shorter thousands and thosuands of times then the muslce as a whole gets shorter, this is one little unit. but if every one little unit gets shorter than macroscopically big picture hte muscle as a whole is getting shorter causing movement of some kind
• edges of sarcomere are defined by Z lines, those red vertical lines at the edges
• within the sarcomere there is myosin which is red in this picture and actin which is blue
• notice top picture and bottom picture: ends/edges moving closer in, those red vertical lines that define the left and right side slide in closer together adn then you have mroe overlap between teh blue and hte red *** the blue itself is not shortening if to measure the length of the red by itself not shortenign just overlapping more
• H band defined in middle by definition, so it is the part where only have myosin with no overlaping actin, so when the contraction which has more overlap occurs then the H band by definition gets shorter* gets smaller and the part within overlap goes away becuase you have mroe overlap* true of I band also shrinks actin blue without overlaping red and that you have less of

whole idea with contraction mroe overlap, and H and I are both defined as regions without much overlap so they shrink*

Question 103

The sliding filaments model (sarcomere contracting)

Answer 103

The sarcomere and the sliding filament model of contraction: During contraction myosin reaches along actin myofilaments compressing the I and H bands. During stretching this tension is release and the I and H bands expand. The A-band remains constant throughout as the length of the myosin myofilaments does not change.

The amount of force and movement generated by an individual sarcomere is small. However, when multiplied by the number of sarcomeres in a myofibril, myofibrils in a myocyte and myocytes in a muscle, the amount of force and movement generated is significant.

When they overlap more many sarcomeres would be contracted and when they loverlap less the sarcomeres and the muscle are relaxed*

Z lines are the end of sarcomere, the boundaries of the sarcomere they shrink as well during contraction, causes a dec in distance btw the z lines of the sarcomere*

During contraction myosin ratchets along actin myofilaments compressing the I and H bands. During stretching this tension is release and the I and H bands expand. The A-band remains constant throughout as the length of the myosin myofilaments does not change.

Question 104

T- tubules details

Answer 104

t-tubules- invaginations or folds in plasma membrane, plasma membrane dips down into the cell but it forms these tubule structures going deeper into the cell which helps action potential which initially going down memraben to be conveyed deeper into the cell*

• those red arrows represent action potentials in image traveling along plasma membrane, and dive down through the t-tubule system, just these kind of like invaginations

NOT MICROTUBULES these are just tunnels**** which allow action potentials to get down to where sarcoplasmic reticiulum is and then voltage changes can cause calcium to be released from SR, then calcium can go and bind to trypoonin and then etc but the action otential needs to get down to where the SR is becuase tha tis where the calcium is, how to think about tubules path action potentials can follow deeper into cell so can trigger next steps in muscle contraction*

this image of neruon synapsing on muscle cell

Question 105

what does innervated mean?

Answer 105

if certain muscle cell is innervated by certain part of nervous system means that is the part of nervous system that sends signals to that muscle cell: so say cardiac muscle is innervated by autonomic nervous system specifically parasympathetic and sympathetic branch, part of nervous system that synapses with cardiac muscle, giving signals to cardiac muscle**

Question 106

Sliding filament model 2

Answer 106

The sarcomere is the region in which sliding filament contraction occurs.

During contraction, myosin myofilaments ratchet over actin myofilaments contracting the sarcomere.

Within the sarcomere, key regions known as the I and H band compress and expand to facilitate this movement.

The myofilaments themselves do not expand or contract.

The sliding filament model describes the process used by muscles to contract. It is a cycle of repetitive events that causes actin and myosin myofilaments to slide over each other, contracting the sarcomere and generating tension in the muscle.

To understand the sliding filament model requires an understanding of sarcomere structure. A sarcomere is defined as the segment between two neighboring, parallel Z-lines. Z lines are composed of a mixture of actin myofilaments and molecules of the highly elastic protein titin crosslinked by alpha-actinin. Actin myofilaments attach directly to the Z-lines, whereas myosin myofilaments attach via titin molecules.

Surrounding the Z-line is the I-band, the region where actin myofilaments are not superimposed by myosin myofilaments. The I-band is spanned by the titin molecule connecting the Z-line with a myosin filament.

The region between two neighboring, parallel I-bands is known as the A-band and contains the entire length of single myosin myofilaments. Within the A-band is a region known as the H-band, which is the region not superimposed by actin myofilaments. Within the H-band is the M-line, which is composed of myosin myofilaments and titin molecules crosslinked by myomesin.

Titin molecules connect the Z-line with the M-line and provide a scaffold for myosin myofilaments. Their elasticity provides the underpinning of muscle contraction. Titin molecules are thought to play a key role as a molecular ruler maintaining parallel alignment within the sarcomere. Another protein, nebulin, is thought to perform a similar role for actin myofilaments.

Question 107

cross bridge cycle

Answer 107

The model of contraction- the molecular mechanism whereby myosin and acting myofilaments slide over each other is termed the cross-bridge cycle. During muscle contraction, the heads of myosin myofilaments quickly bind and release in a ratcheting fashion, pulling themselves along the actin myofilament.

At the level of the sliding filament model, expansion and contraction only occurs within the I and H-bands. The myofilaments themselves do not contract or expand and so the A-band remains constant.

Question 108

Thin and thick filaments are organized into functional units called ___

Answer 108

sarcomeres