Ebola Vaccines

Question 1

Clinical feature of Ebola

Answer 1

• high viral replication
• high fever
• vomiting, diarrhoea
• impaired coagulation
• thrombocytopenia

Question 2

First Ebola outbreak

Answer 2

Zaire 1976

Question 3

Species of Ebola virus

Answer 3

• Zaire
• Sudan
• Cote d’ Ivoire
• Reston
• Bundibugyo

Question 4

Type of vaccine platform for Ebola

Answer 4

• rAd
• VSV
• DNA/rAd
• HPIV3
• VLP

Question 5

Primary Ebola vaccine target

Answer 5

Surface glycoprotein GP

Question 6

2 aims for vaccines

Answer 6

• identify prime that provides uniform protection against acute challenge
• define single-shot or prime-boost regimen for durable immunity

Question 7

Correlate of protection in rAD vaccine

Answer 7

• antibody

Question 8

Mechanism of protection in rAd5 vaccine

Answer 8

CD8 T cells

Question 9

How we are able to tell that CD8 T cells are the mechanism of protection

Answer 9

• poor protection with Ab transfer
• loss of protection with CD8 depletion
• cross-strain protection with CD8 response
• loss of protection with pre-existing vector immunity track with T cell response

Question 10

Best vector to boost ChAd3 for cellular immune response?

Answer 10

3/MVA

Question 11

What does VSV stand for?

Answer 11

vesicular stomatitis virus

Question 12

Characteristics of VSV

Answer 12

• prototypic rhabdovirus
• growth to high titres
• strong induction of IS
• low levels of pre-existing immunity in population
• low level transient viraemia