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BacT RUSVM Summer 15 > E. Coli, general and pigs > Flashcards

E. Coli, general and pigs Flashcards

1
Q

E. coli is a member of the Enterobacteriaceae family, what are the benefits of this family within the normal flora?

A

Microbial antagonism
Breakdown and absorption of food
Waste Processing
Vit. K production

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2
Q

What is unique to gram negative Bacteria?

A

LPS

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3
Q

Gram - BacT Lipid A causes?

A

Endotoxic shock

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4
Q

General facts about Enterobacteriaceae

A 
40 Genera, 180 species
Gram -
Facultative anaerobic
Rods or coccobacilli
Oxidase - (except Plesiomonas, shigelloids)
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5
Q

Klebsiella pneumonia, oxytoca
Enterobacter cloacae, aerogenes
Proteus mirabilis
Citrobacter freundii

All of these are common with?

A

MOST COMMON NOSOCOMIAL/ Health care associated infections with ICU patients.

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6
Q

What all species of Escherichia are clinically relevant?

A

E. coli, period, thats it its underlined in the notes go check ill wait!

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7
Q

With regards to lactose and oxidase tests how does E. Coli react?

A

Positive and negative respectfully

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8
Q

What is the morphology and motility of E. coli?

A

Motile Rods

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9
Q

Serotype definition?

A

Complete antigenic formula of an organism

ex. E. coli 0:26, K:60 (B6), F: 41, H: 11

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10
Q

What does the O mean in the serotyping?

A

O antigen

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11
Q

H in serotyping refers to?

A

flagella

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12
Q

F in serotyping refers to

A

Fimbriae

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13
Q

K in serotyping refers to?

A

Capsule

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14
Q

Shigella spp. are taxonomically also Escherichia? T/F?

A

True but shigella not present in animals

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15
Q

What are the general E. coli infections?

A 
Intestinal pathogenic strain
extra intestinal pathogenic strains (ExPEC).
-Respiratory
-Septicemia
-UTI
-Mastitis
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16
Q

What is the abbreviation ExPEC?

A

Extra intestinal pathogenic strains

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17
Q

what are the cell associated virulence factors associated with E. coli?

A

Endotoxin
Capsule
Fimbrial adhesins
Non-fimbrial adhesins

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18
Q

What are the extracellular virulence factors associated with E. coli?

A

Enterotoxins
Cytotoxins
Siderophores ( capture iron, ExPEC)

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19
Q

I’ll take 4 letter abbreviations for 500 alex.

A

You lucky ross student got the daily double enjoy the next few cards!

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20
Q

ETEC?

A

enterotoxigenic

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21
Q

EHEC?

A

Enterohemorrhagic

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22
Q

VTEC/STEC - DAILY DOUBLE

A

Verotoxigenic/ Shiga-like toxic

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23
Q

EPEC

A

Enteropahogenic

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24
Q

RPEC

A

Rabbit pathogenic- dont ask me why rabbits are so special i do not know why

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25
Q

Human abbreviations EAEC, DAEC, EICE, EAggEC

A

Enteroadherent
Diffusely adherent
Enteroinvasive
Enteroadherent aggregative

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26
Q

APEC

A

Avian pathogenic E. coli

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27
Q

NTEC

A

necrotoxigenic- Cattle

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28
Q

Mastitis Causing E. coli- animal species?

A

bovine

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29
Q

MMA which species?

A

mastitis in pigs

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30
Q

Swine and dine me with these types E. coli that ruin the bacon machines ( what types of E. coli are going to give pigs a bad time)

A 
ETEC- enterotoxigenic
VTEC- verotoxigenic/ shiga-like toxic
EPEC-enterpathogenic
UTI
MMA- mastitis not fighting
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31
Q

Whats the beef with cattle and E. coli types?

A 
ETEC-??? GOT IT YET?!
EHEC
EPEC
Mastitis
Septicemia
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32
Q

E. coli of importance in Vetmed with avian species?

A

APEC

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33
Q

Rabbits E. coli

A

RPEC

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34
Q

DDx: for pigglets with diarrhea?

A 
ETEC
EPEC
Clostridium perfringens: entertoxemia
Clostridium difficile
Brachyspira hydosenteriae, pilosicoli
Lawsonia intracellularis
Salmonella (non-host specific serotypes)
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35
Q

DDx: Diarrhea in fattening pigs, sows and boars?

A

NOT E. coli!
brachyspira hyodysenteriae, pilosicoli
lawsonia intracllularis
Salmonella (non-host specific)

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36
Q

ETEC in pigs cause what clinical signs?

A

Watery diarrhea

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37
Q

ETEC in pigs is usually at what age?

A

neonatal/2-4wks/weaning

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38
Q

What are the adhesion factors for ETEC in pigs? (Small Intestine)

A

F4 (K88); F5 (K99); F6 (987P); F18; F41

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39
Q

What are the enterotoxins for ETEC in pigs and where in the genome are they located?

A

LT (heat labile)
Sta, STb, EAST1 (Heat stable)
Genetic coding on plasmids

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40
Q

F4 (K88) adhesion factor, the genetic coding for this is found in the the chromosome or a plasmid?

A

Plasmid

41
Q

F4 (K88) adhesion factor is resistant to what?

A

Mannose

42
Q

When are genes for F4 (K88) Expressed?

A

At body temp not room Temp

43
Q

What species is F4 (K88) Found

A

Swine only

44
Q

F5 (K99) chromosome or plasmid?

A

plasmid

45
Q

F5 K99 species?

A

swine/bovine

46
Q

F5 K99- what is it?

A

Fimbrial adhesion factor for small intestine

47
Q

F5 K99- resistant to?

A

mannose

48
Q

F4 K88 Receptor?

A

Specific –> Breeding of receptor free pigs, Age dependent presence

49
Q

F5 K99 gene expression is dependent or independent of temperature?

A

dependent

50
Q

What is unique about the receptor for F5 K99

A

Only in very young animals, 1-3 days old involved in neonatal Diarrhea

51
Q

F6 987P is found where in the genetics and in what age animals?

A

Mainly extrachromosomal and in neonatal animals- thats all thats listed about this Fimbrial adhesion factor…

52
Q

F41 is chomosomal or plasmid?

A

CHROMOSOMAL!!!!!!!!!

53
Q

F41 is found in which species

A

Swine/ Bovine but is rare and not seen often

54
Q

F41 is usually coupled with what other fimbrial adhesion factor?

A

F5

55
Q

What is the non-fimbrial adhesin?

A

AIDA-1 (involved in diffuse adherenece)

56
Q

Enterotoxins of E. coli (on plasmids) what are the details?

A

Heat labile

  • LT
  • High Molecular weight ( GOOD ANTIGEN**)
  • Inactivated 30 min @ 60oC
  • DEC Na absorbtion
  • INC Cl- and Bicarb secretion
57
Q

STa is what?

A

Sub type A enterotoxin

58
Q

what does STa do?

A

Activateds Guanylate cyclase system

  • **DEC Na absorbtion
  • ** INC Cl- & Bicarb secretion
59
Q

STb is what?

A

Sub Type B enterotoxin

60
Q

What does STb Do?

A

Prostaglandin E2 secretion leading to secretion of water and electrolytes
Stimulation of 5-hydrotryptamine secretion

61
Q

EAST1?

A

Enteroaggregative E. coli heat stable toxin

62
Q

EAST1 is closely related to?

A

STa

63
Q

What Is important about EAST1?

A

Found in ETEC, EPEC, AEEC, VETC
LOW MOLECULAR WEIGHT- POOR ANTIGEN
Withstand 15 min @ 121oC

64
Q

Neonatal ETEC is associated with what characteristics?

A

F5, F6, F41
STa, STb
non-hemolytic

NOT all of the factors have to be present, these are just common associated factors.

65
Q

Neonatal till 4 weeks is associated with which characteristics?

A

F4
LT, STa, STb, EAST1
HEMOLYTIC

NOT all of the factors have to be present, these are just common associated factors.

66
Q

Waning Diarrhea is associated with which characteristics?

A

F4, F18
LT, STa, STb, EAST1
Hemolytic

NOT all of the factors have to be present, these are just common associated factors.

67
Q

The epidemiology of E. coli is something of importance. How is the disease spread.

A

Equilibrium between maternal immunity andy infection pressure is altered.
caused by poor hygiene and presence of ETEC diseased pigs

68
Q

Pathogenesis of ETEC

A

Asending from lower bowel
Oral uptake
Small intestine
colonization.

-Adhesion
-XXX
-Enterotoxins
-Evt. Secondary septicemia
Thats how it is in the notes

69
Q

Symptoms of ETEC

A
  • patients remain drinking
  • Very stinky shit
  • Dehydration
  • Older animals get the Diarrhea
    • white/grey not as watery
70
Q

Diagnosis of ETEC in pigs?

A

Detection of virulence factors
Agglutination Tests
PCR

71
Q

Treatment of ETEC in pigs?

A

Supportive care- Fluids (SQ, IP) (electrolytes, glucose &AAs)

72
Q

Prevention of ETEC in pigs?

A

Hygiene
Vaccination
-immunize sows
-different F factors are at different ages know which ages you are wanting to vaccinate.

73
Q

Edema Dz in pigs abbreviation?-

A

VTEC

74
Q

What age group is VTEC? Porcine

A

post weaning

75
Q

Virulence factors for porcine VTEC

A

Adhesion (F18) (specific receptor, resistant if absent)
Exotoxin
-VT2e or Stx2e: Media necrosis
Endotoxin- Acute mortality

76
Q

Epi of VTEC in pork?

A 
Sow to Piglet
Weaning:
-Stress
-Change in flora
-Maternal immunity

Excretion
Inc Infection pressure leading to other pigs getting infected

77
Q

Hyperacute toxin production in VTEC - pigs

A

Enterorrhagia

78
Q

Acute toxin production VTEC- Pigs

A

Permeability blood vessel edema (CNS & dyspnea)

79
Q

Slower Toxin production VTEC- Pigs

A

Intravascular Coagulation (CNS)

80
Q

Endotoxin production VTEC results in

A

Shock and acute mortality

81
Q

VTEC- Pigs Symptoms

A
82
Q

Dx of VTEC- pigs

A

Clinical signs
Pathological findings
Bacteriology (Fecal Sample)
PCR

83
Q

Tx of VTEC- affected Pigs

A

Fast them

Antimicrobial therapy- do sensitivity prior

84
Q

Tx of VTEC- non affected pigs

A

Antimicrobials- incubation of toxin 2-3 days (symptoms after Tx)

85
Q

Prevention VTEC

A

Reduce stress
Feed composition
Adapt good intestinal flora (Probiotics, Organic acids, Zinc oxide >2400ppm kills E. coli, Antimicrobials, Vaccination VT2e)
Selection of receptor F18 negative animals

86
Q

EPEC pigs is what?

A

Enteropathogenic E. coli

87
Q 
EPEC in pigs: 
How common
What are the signs
What does it do
whats the vaccine
and whats the Dx procedure?
A 
More rare
Diarrhea at weaning
Attaching and effacing
T3SS and injection of effector proteins
Isolation + PCR (eae(intimin) gene)

only 1 slide on this whole disease

88
Q

UTI, PIGS, GO!

A

E. coli most important PATHOGEN IN UTI IN PIGS AND OTHER MAMMALS!

89
Q

Ddx of swine UTI

A

Actinobaculum suis

90
Q

Where does the bacteria originate from in swine UTI?

A

Notes Say from intestine (Poopin on your pee’r)

91
Q

Symptoms of UTI- E. coli in pigs

A

Anorexia
Hematuria
Vaginal Exudate (MOIST)
general malaise

92
Q

Dx of UTI E. coli Pigs

A

Bacteriology (semi-quantititave from 10^3 to 10^5)

93
Q

Tx of UTI in pigs E. coli

A

Culling (Fertility problems)

Antibiotics 3wks

94
Q

MMA

A

metritis-mastitis-agalactiae

95
Q

PPDS

A

Post Partum Dysgalactiae syndrome

96
Q

Etiology of MMA of PPDS in pigs?

A

Hereditary
Hormonal
Feed
Infections agents: E. coli

97
Q

DDx: MMA of PPDS pigs

A

Klbsiella, Enterobacter, Citrobacter

98
Q

Septicemia via E. coli in pigs

A

Rarely primary Dz, Secondary to enteric infection

BacT RUSVM Summer 15 (20 decks)

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