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BacT RUSVM Summer 15 > E. Coli, general and pigs > Flashcards

E. Coli, general and pigs Flashcards

1
Q

E. coli is a member of the Enterobacteriaceae family, what are the benefits of this family within the normal flora?

A

Microbial antagonism
Breakdown and absorption of food
Waste Processing
Vit. K production

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2
Q

What is unique to gram negative Bacteria?

A

LPS

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3
Q

Gram - BacT Lipid A causes?

A

Endotoxic shock

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4
Q

General facts about Enterobacteriaceae

A 
40 Genera, 180 species
Gram -
Facultative anaerobic
Rods or coccobacilli
Oxidase - (except Plesiomonas, shigelloids)
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5
Q

Klebsiella pneumonia, oxytoca
Enterobacter cloacae, aerogenes
Proteus mirabilis
Citrobacter freundii

All of these are common with?

A

MOST COMMON NOSOCOMIAL/ Health care associated infections with ICU patients.

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6
Q

What all species of Escherichia are clinically relevant?

A

E. coli, period, thats it its underlined in the notes go check ill wait!

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7
Q

With regards to lactose and oxidase tests how does E. Coli react?

A

Positive and negative respectfully

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8
Q

What is the morphology and motility of E. coli?

A

Motile Rods

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9
Q

Serotype definition?

A

Complete antigenic formula of an organism

ex. E. coli 0:26, K:60 (B6), F: 41, H: 11

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10
Q

What does the O mean in the serotyping?

A

O antigen

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11
Q

H in serotyping refers to?

A

flagella

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12
Q

F in serotyping refers to

A

Fimbriae

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13
Q

K in serotyping refers to?

A

Capsule

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14
Q

Shigella spp. are taxonomically also Escherichia? T/F?

A

True but shigella not present in animals

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15
Q

What are the general E. coli infections?

A 
Intestinal pathogenic strain
extra intestinal pathogenic strains (ExPEC).
-Respiratory
-Septicemia
-UTI
-Mastitis
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16
Q

What is the abbreviation ExPEC?

A

Extra intestinal pathogenic strains

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17
Q

what are the cell associated virulence factors associated with E. coli?

A

Endotoxin
Capsule
Fimbrial adhesins
Non-fimbrial adhesins

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18
Q

What are the extracellular virulence factors associated with E. coli?

A

Enterotoxins
Cytotoxins
Siderophores ( capture iron, ExPEC)

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19
Q

I’ll take 4 letter abbreviations for 500 alex.

A

You lucky ross student got the daily double enjoy the next few cards!

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20
Q

ETEC?

A

enterotoxigenic

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21
Q

EHEC?

A

Enterohemorrhagic

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22
Q

VTEC/STEC - DAILY DOUBLE

A

Verotoxigenic/ Shiga-like toxic

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23
Q

EPEC

A

Enteropahogenic

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24
Q

RPEC

A

Rabbit pathogenic- dont ask me why rabbits are so special i do not know why

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25
Human abbreviations EAEC, DAEC, EICE, EAggEC
Enteroadherent Diffusely adherent Enteroinvasive Enteroadherent aggregative
26
APEC
Avian pathogenic E. coli
27
NTEC
necrotoxigenic- Cattle
28
Mastitis Causing E. coli- animal species?
bovine
29
MMA which species?
mastitis in pigs
30
Swine and dine me with these types E. coli that ruin the bacon machines ( what types of E. coli are going to give pigs a bad time)
``` ETEC- enterotoxigenic VTEC- verotoxigenic/ shiga-like toxic EPEC-enterpathogenic UTI MMA- mastitis not fighting ```
31
Whats the beef with cattle and E. coli types?
``` ETEC-??? GOT IT YET?! EHEC EPEC Mastitis Septicemia ```
32
E. coli of importance in Vetmed with avian species?
APEC
33
Rabbits E. coli
RPEC
34
DDx: for pigglets with diarrhea?
``` ETEC EPEC Clostridium perfringens: entertoxemia Clostridium difficile Brachyspira hydosenteriae, pilosicoli Lawsonia intracellularis Salmonella (non-host specific serotypes) ```
35
DDx: Diarrhea in fattening pigs, sows and boars?
NOT E. coli! brachyspira hyodysenteriae, pilosicoli lawsonia intracllularis Salmonella (non-host specific)
36
ETEC in pigs cause what clinical signs?
Watery diarrhea
37
ETEC in pigs is usually at what age?
neonatal/2-4wks/weaning
38
What are the adhesion factors for ETEC in pigs? (Small Intestine)
F4 (K88); F5 (K99); F6 (987P); F18; F41
39
What are the enterotoxins for ETEC in pigs and where in the genome are they located?
LT (heat labile) Sta, STb, EAST1 (Heat stable) Genetic coding on plasmids
40
F4 (K88) adhesion factor, the genetic coding for this is found in the the chromosome or a plasmid?
Plasmid
41
F4 (K88) adhesion factor is resistant to what?
Mannose
42
When are genes for F4 (K88) Expressed?
At body temp not room Temp
43
What species is F4 (K88) Found
Swine only
44
F5 (K99) chromosome or plasmid?
plasmid
45
F5 K99 species?
swine/bovine
46
F5 K99- what is it?
Fimbrial adhesion factor for small intestine
47
F5 K99- resistant to?
mannose
48
F4 K88 Receptor?
Specific --> Breeding of receptor free pigs, Age dependent presence
49
F5 K99 gene expression is dependent or independent of temperature?
dependent
50
What is unique about the receptor for F5 K99
Only in very young animals, 1-3 days old involved in neonatal Diarrhea
51
F6 987P is found where in the genetics and in what age animals?
Mainly extrachromosomal and in neonatal animals- thats all thats listed about this Fimbrial adhesion factor...
52
F41 is chomosomal or plasmid?
CHROMOSOMAL!!!!!!!!!
53
F41 is found in which species
Swine/ Bovine but is rare and not seen often
54
F41 is usually coupled with what other fimbrial adhesion factor?
F5
55
What is the non-fimbrial adhesin?
AIDA-1 (involved in diffuse adherenece)
56
Enterotoxins of E. coli (on plasmids) what are the details?
Heat labile - LT - High Molecular weight ( GOOD ANTIGEN**) - Inactivated 30 min @ 60oC - DEC Na absorbtion - INC Cl- and Bicarb secretion
57
STa is what?
Sub type A enterotoxin
58
what does STa do?
Activateds Guanylate cyclase system - **DEC Na absorbtion - ** INC Cl- & Bicarb secretion
59
STb is what?
Sub Type B enterotoxin
60
What does STb Do?
Prostaglandin E2 secretion leading to secretion of water and electrolytes Stimulation of 5-hydrotryptamine secretion
61
EAST1?
Enteroaggregative E. coli heat stable toxin
62
EAST1 is closely related to?
STa
63
What Is important about EAST1?
Found in ETEC, EPEC, AEEC, VETC LOW MOLECULAR WEIGHT- POOR ANTIGEN Withstand 15 min @ 121oC
64
Neonatal ETEC is associated with what characteristics?
F5, F6, F41 STa, STb non-hemolytic NOT all of the factors have to be present, these are just common associated factors.
65
Neonatal till 4 weeks is associated with which characteristics?
F4 LT, STa, STb, EAST1 HEMOLYTIC NOT all of the factors have to be present, these are just common associated factors.
66
Waning Diarrhea is associated with which characteristics?
F4, F18 LT, STa, STb, EAST1 Hemolytic NOT all of the factors have to be present, these are just common associated factors.
67
The epidemiology of E. coli is something of importance. How is the disease spread.
Equilibrium between maternal immunity andy infection pressure is altered. caused by poor hygiene and presence of ETEC diseased pigs
68
Pathogenesis of ETEC
Asending from lower bowel Oral uptake Small intestine colonization. -Adhesion -XXX -Enterotoxins -Evt. Secondary septicemia Thats how it is in the notes
69
Symptoms of ETEC
- patients remain drinking - Very stinky shit - Dehydration - Older animals get the Diarrhea - * white/grey not as watery
70
Diagnosis of ETEC in pigs?
Detection of virulence factors Agglutination Tests PCR
71
Treatment of ETEC in pigs?
Supportive care- Fluids (SQ, IP) (electrolytes, glucose &AAs)
72
Prevention of ETEC in pigs?
Hygiene Vaccination -immunize sows -different F factors are at different ages know which ages you are wanting to vaccinate.
73
Edema Dz in pigs abbreviation?-
VTEC
74
What age group is VTEC? Porcine
post weaning
75
Virulence factors for porcine VTEC
Adhesion (F18) (specific receptor, resistant if absent) Exotoxin -VT2e or Stx2e: Media necrosis Endotoxin- Acute mortality
76
Epi of VTEC in pork?
``` Sow to Piglet Weaning: -Stress -Change in flora -Maternal immunity ``` Excretion Inc Infection pressure leading to other pigs getting infected
77
Hyperacute toxin production in VTEC - pigs
Enterorrhagia
78
Acute toxin production VTEC- Pigs
Permeability blood vessel edema (CNS & dyspnea)
79
Slower Toxin production VTEC- Pigs
Intravascular Coagulation (CNS)
80
Endotoxin production VTEC results in
Shock and acute mortality
81
VTEC- Pigs Symptoms
82
Dx of VTEC- pigs
Clinical signs Pathological findings Bacteriology (Fecal Sample) PCR
83
Tx of VTEC- affected Pigs
Fast them | Antimicrobial therapy- do sensitivity prior
84
Tx of VTEC- non affected pigs
Antimicrobials- incubation of toxin 2-3 days (symptoms after Tx)
85
Prevention VTEC
Reduce stress Feed composition Adapt good intestinal flora (Probiotics, Organic acids, Zinc oxide >2400ppm kills E. coli, Antimicrobials, Vaccination VT2e) Selection of receptor F18 negative animals
86
EPEC pigs is what?
Enteropathogenic E. coli
87
``` EPEC in pigs: How common What are the signs What does it do whats the vaccine and whats the Dx procedure? ```
``` More rare Diarrhea at weaning Attaching and effacing T3SS and injection of effector proteins Isolation + PCR (eae(intimin) gene) ``` only 1 slide on this whole disease
88
UTI, PIGS, GO!
E. coli most important PATHOGEN IN UTI IN PIGS AND OTHER MAMMALS!
89
Ddx of swine UTI
Actinobaculum suis
90
Where does the bacteria originate from in swine UTI?
Notes Say from intestine (Poopin on your pee'r)
91
Symptoms of UTI- E. coli in pigs
Anorexia Hematuria Vaginal Exudate (MOIST) general malaise
92
Dx of UTI E. coli Pigs
Bacteriology (semi-quantititave from 10^3 to 10^5)
93
Tx of UTI in pigs E. coli
Culling (Fertility problems) | Antibiotics 3wks
94
MMA
metritis-mastitis-agalactiae
95
PPDS
Post Partum Dysgalactiae syndrome
96
Etiology of MMA of PPDS in pigs?
Hereditary Hormonal Feed Infections agents: E. coli
97
DDx: MMA of PPDS pigs
Klbsiella, Enterobacter, Citrobacter
98
Septicemia via E. coli in pigs
Rarely primary Dz, Secondary to enteric infection

BacT RUSVM Summer 15 (20 decks)

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