DNA TECHNOLOGIE Flashcards
1
Q
why is it harder to tramslate the genome of a complex organism
A
- contains portions of noncoding dna
- contain regulatory genes which switch genes for some proteins on and off
2
Q
what is recombinant DNA technology
A
transfering DNA fragment from one organism to another to see protein made
3
Q
describe how a DNA fragment can be made using reverse transcriptase
A
mRNA isolated from cells
reverse transcriptase uses mrna as template to synthesize new strand of cDNA
4
Q
describe how DNA fragments made using restriction endonuclease enzymes
A
restriction endonuclease is enzyme specific to pallindromic dna sequences
