DNA replication

Question 1

Antiparallel

Answer 1

(DNA strands) meaning that they run in opposite directions to one another

Question 2

5’ and 3’

Answer 2

The 5 and 3 direction of elongation. There is always one side that is lagging and one that is leading. (Happens after polymerase the one that replicates)

Question 3

Replicating worker: Helicase

Answer 3

The enzyme that binds to a specific sequence of DNA is referred to as replication origin.

They cut and unwind the Double Helix.

(Becomes template for next steps)

Question 4

Replicating worker: Primase

Answer 4

Enzyme to sequence primers. (Removed when replication is complete)

Question 5

Replicating worker: Polymerase

Answer 5

Begins to add nucleotides creating two new strands of DNA.

Question 6

Replicating worker: Ligase

Answer 6

Helps the legging strand to be intertwined with the leading strand. (End of replication process)

Question 7

Restriction enzyme

Answer 7

They (cleave) split DNA molecules at the specific sequence base. (first part of gel ectophyte)

Question 8

Gel electrolyte

Answer 8

1) DNA is split with restriction enzyme at the base
2) DNA is negative so migrates toward a positive charge
3) The banding pattern shows and separates the coding of the DNA.