DNA/Genes Flashcards
Semi-Conservative
One daughter molecule is an exact replica of the parent, one is entirely new material
Conservative
The parental double helix remains fully intact in daughter molecules
Dispersive
Each strand of daughter molecule contains a mixture of new and old parts
DNA strands run…
antiparallel
DNA synthesis has…
directionality (we can only add to the 3’ end, not the 5’)
DNA is synthesized in a ____ manner
5’—>3’
Helicase
Breaks H-bonds to open the replication bubble.
Single-strand DNA-binding proteins
Stabilizes single-stranded DNA (keeps DNA single-stranded)
Topoisomerase
Breaks and rejoins the DNA double helix to relieve twisting forces caused by the opening of the helix
What catalyzes DNA synthesis?
DNA polymerase
Primers are _____ that allow the initiation of ___
short sequences; DNA synthesis
Which part of a deoxynucleoside triphosphate (dNTP) molecule provides the energy for DNA synthesis?
Phosphate groups
Which enzyme creates a primer for DNA polymerase?
Primase
Okazaki fragments in E. coli are formed _____
on the lagging strand of DNA
Which of the following hypothetical changes would eliminate the need to synthesize a lagging strand?
Allowing DNA synthesis to occur in both the 5’ to 3’ and 3’ to 5’ directions
____ stretches of single-stranded DNA are expected on the lagging strand template than on the leading strand template.
Longer
Apoptosis
Cell becomes destroyed (it dies)
We know how old a cell is based off of….
How short it’s telomeres are
why does apoptosis occur!
If left, cells can become cancerous over time
Interphsses
G1: Cell growth
S: DNA REPLICATION
G2: ??
Mitosis is division of ____ cells
Somatic
Major roles of mitosis
Organismal growth
Development
Tissue repair/renewal
Reproduction
Mitosis is highly ……
Regulated
Homologous chromosomes
Same genes in the same order, don’t always have the same DNA sequence
Each chromatid contains ______
A single molecule of double-stranded DNA
Each chromatid contains the same _____
set of genes
Each chromosome contains both ______
DNA and protein
Stages of mitosis in order
Prophase
Prometaphase
Metaphase
Anaphase
Telophase
Cytokinesis
During which stages do microtubules attach to kinetochores?
prometaphase
During which stage do tubulins assemble into spindle microtubules?
prophase
During which stage do cohesions break down?
anaphase
During which stage do kinetochores become motionless in relation to pores of the cell?
metaphase
During which stage do kinetochores move toward poles of the cell?
anaphase
During which stage do spindle microtubules disassemble?
telophase
During which stage do cohesins join sister chromatids of duplicated chromosomes?
During prophase, the microtubules of the mitotic spindle
lengthen
During anaphase, the nonkinetochore microtubules _____ and move past each other, and the kinetochore microtubules_____
lengthen, shorten
During telophase, the nonkinetochore microtubules
disassemble